Mouse SPARC Antibody

Catalog # Availability Size / Price Qty
MAB942
MAB942-SP
Mouse SPARC Antibody in Western Blot
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Mouse SPARC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SPARC/Osteonectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SPARC is observed.
Source
Monoclonal Rat IgG2B Clone # 124413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin
Ala18-Ile302
Accession # P07214
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Balb/3T3 mouse embryonic fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse SPARC by Western Blot. View Larger

Detection of Mouse SPARC by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse SPARC Monoclonal Antibody (Catalog # MAB942) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry SPARC/Osteonectin in Mouse Ovary. View Larger

SPARC/Osteonectin in Mouse Ovary. SPARC/Osteonectin was detected in perfusion fixed frozen sections of mouse ovary using Mouse SPARC/Osteonectin Monoclonal Antibody (Catalog # MAB942) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPARC

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1‑5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1‑5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3‑5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3‑5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92% and 83% aa identity with rat, human, dog, cow and chick SPARC, respectively.

References
  1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
  2. McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
  3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
  4. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  5. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
  6. Hohenester, E. et al. (1997) EMBO J. 16:3778.
  7. Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
  8. Delany, A.M. et al. (2003) Endocrinology 144:2588.
  9. Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
  10. Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
  11. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.
Long Name
Secreted Protein Acidic and Rich in Cysteine
Entrez Gene IDs
6678 (Human); 20692 (Mouse)
Alternate Names
Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC

Product Datasheets

Citations for Mouse SPARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Implication of SPARC in the modulation of the extracellular matrix and mitochondrial function in muscle cells
    Authors: A Melouane, A Carbonell, M Yoshioka, J Puymirat, J St-Amand
    PLoS ONE, 2018;13(2):e0192714.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. SPARC Expression Did Not Predict Efficacy of nab-Paclitaxel plus Gemcitabine or Gemcitabine Alone for Metastatic Pancreatic Cancer in an Exploratory Analysis of the Phase III MPACT Trial.
    Authors: Hidalgo M, Plaza C, Musteanu M, Illei P, Brachmann C, Heise C, Pierce D, Lopez-Casas P, Menendez C, Tabernero J, Romano A, Wei X, Lopez-Rios F, Von Hoff D
    Clin Cancer Res, 2015;21(21):4811-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Clever-1/stabilin-1 controls cancer growth and metastasis.
    Authors: Karikoski M, Marttila-Ichihara F, Elima K, Rantakari P, Hollmen M, Kelkka T, Gerke H, Huovinen V, Irjala H, Holmdahl R, Salmi M, Jalkanen S
    Clin Cancer Res, 2014;20(24):6452-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. SPARC mediates early extracellular matrix remodeling following myocardial infarction.
    Authors: McCurdy S, Dai Q, Zhang J, Zamilpa R, Ramirez T, Dayah T, Nguyen N, Jin Y, Bradshaw A, Lindsey M
    Am J Physiol Heart Circ Physiol, 2011;301(2):H497-505.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Secreted protein acidic and rich in cysteine deficiency ameliorates renal inflammation and fibrosis in angiotensin hypertension.
    Authors: Socha MJ, Manhiani M, Said N, Imig JD, Motamed K
    Am. J. Pathol., 2007;171(4):1104-12.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Absence of SPARC in lens epithelial cells results in altered adhesion and extracellular matrix production in vitro.
    Authors: Weaver MS, Sage EH, Yan Q
    J. Cell. Biochem., 2006;97(2):423-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse SPARC Antibody

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Mouse SPARC Antibody
By Anonymous on 01/09/2018
Application: ELISA Sample Tested: Recombinant protein Species: Human