Mouse TLR4 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TLR4 in direct ELISAs. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4.
  • Source
    Monoclonal Rat IgG2A Clone # 267518
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TLR4
    Asn26-Lys629
    Accession # Q9QUK6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, fixed with paraformaldehyde and permeabilized with saponin
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TLR4 in RAW 264.7 Mouse Monocyte/Macrophage Cell Line. TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/
macrophage cell line using Rat Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB2759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the
NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

  • Long Name:
    Toll-like Receptor 4
  • Entrez Gene IDs:
    7099 (Human); 21898 (Mouse); 29260 (Rat)
  • Alternate Names:
    ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; TLR4; TOLL; toll-like receptor 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Absence of toll-like receptor 4 (TLR4) extends survival in the hSOD1 G93A mouse model of amyotrophic lateral sclerosis.
    Authors: Lee J, Lee J, Phipps S, Noakes P, Woodruff T
    J Neuroinflammation, 2015;12(0):90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  2. Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
    Authors: Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P
    , 2014;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Adjuvant effect of killed Propionibacterium acnes on mouse peritoneal B-1 lymphocytes and their early phagocyte differentiation.
    Authors: Mussalem JS, Squaiella-Baptistao CC, Teixeira D, Yendo TM, Thies FG, Popi AF, Mariano M, Longo-Maugeri I
    PLoS ONE, 2012;7(3):e33955.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Toll-like receptor 4 regulates early endothelial activation during ischemic acute kidney injury.
    Authors: Chen J, John R, Richardson JA, Shelton JM, Zhou XJ, Wang Y, Wu QQ, Hartono JR, Winterberg PD, Lu CY
    Kidney Int., 2011;79(3):288-99.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2.
    Authors: Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA, Fan J
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(6):R1670-80.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
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