Mouse TLR9 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TLR-9 in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1138D
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse TLR9
    Leu26-Asp818
    Accession # AAK29625
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunocytochemistry
    0.5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TLR9 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rabbit Anti-Mouse TLR9 Monoclonal Antibody
(Catalog # MAB7960) or (B) Normal Rabbit IgG Control
(Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). 
Immunocytochemistry
TLR9 in Mouse Splenocytes. TLR9 was detected in immersion fixed mouse splenocytes using Rabbit Anti-Mouse TLR9 Monoclonal Antibody (Catalog # MAB7960) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR9

TLR9 (Toll‑like receptor 9), designated CD289, is a member of the TLR family of innate immune receptors that is mainly expressed by colonic epithelium, CD123+ plasmacytoid predendritic cells (pDC), and splenic transitional B cells (1‑9). TLR9 responds to unmethylated DNA CpG motifs that occur mainly in bacteria and viruses (1, 2). Mouse TLR9 cDNA encodes a 1032 amino acid (aa) type I transmembrane glycoprotein with a 793 aa extracellular domain (ECD) that contains 26 leucine‑rich repeats (LRRs, aa 26‑818), and a 193 aa cytoplasmic domain with a TIR sequence that dimerizes with signaling adaptors such as MyD88 (1). The mouse TLR9 ECD shares 87% aa sequence identity with rat and 71‑74% with human, feline, canine, equine, porcine, bovine and ovine TLR9. Predicted splice forms vary at the N‑terminus by initiating either upstream or downstream of the standard site. The full-length 150 kDa form, which is ligand‑binding but nonsignaling, is found in the endoplasmic reticulum. It undergoes accessory protein-mediated translocation either to the cell membrane or to lysosomes (1‑3). TLR9 is cleaved to remove LRR1‑14, producing an 80 kDa signaling fragment within acidic endolysosomes where it encounters microbial CpG DNA rather than self-DNA (2, 10, 11). However, immune complexes of self‑DNA with lupus erythematosus anti‑DNA antibodies can induce TLR9 activation and IFN‑ alpha production in pDC (4). A soluble form also found in endosomes includes all 26 LRRs and negatively regulates active TLR9 (12). Activation of TLR9 contributes to splenocyte proliferation, pDC maturation, macrophage inflammatory cytokine production, Th1 inflammatory responses, NK cell activation and recruitment, B cell surface MHC class II up‑regulation and immunoglobulin production, and generation and maintenance of memory B cells (1, 5‑9).

  • References:
    1. Hemmi, H. et al. (2000) Nature 408:740.
    2. Barton, G.M. et al. (2006) Nat. Immunol. 7:49.
    3. Latz, E. et al. (2004) Nat. Immunol. 5:190.
    4. Barrat, F.J. et al. (2005) J. Exp. Med. 202:1131.
    5. Krieg, A.M. et al. (1995) Nature 374:546.
    6. Aranburu, A. et al. (2010) J. Immunol. 185:7293.
    7. Guerrier, T. et al. (2012) J. Autoimmun. 39:173.
    8. Guillerey, C. et al. (2012) Blood 120:90.
    9. Cunningham-Rundles, C. et al. (2006) J. Immunol. 176:1978.
    10. Ewald, S.E. et al. (2008) Nature 456:658.
    11. Park, B. et al. (2008) Nat. Immunol. 9:1407.
    12. Chockalingam, A. et al. (2011) Eur. J. Immunol. 41:2176.
  • Long Name:
    Toll-like Receptor 9
  • Entrez Gene IDs:
    54106 (Human); 81897 (Mouse); 338457 (Rat)
  • Alternate Names:
    CD289 antigen; CD289; TLR9; toll-like receptor 9
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Donkey Anti-Rabbit IgG (H+L) Antibody

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