Recombinant Mouse TREM‑1 Fc Chimera (Catalog # 1187-TR)
0.25 µg/106 cells
Measured by its ability to stimulate TNF-alpha secretion by P388D1 mouse lymphoma cells (ATCC # TIB-63). Bouchon, A. et al. (2001) Nature, 410:1103 and Bouchon, A. et al. (2000) J. Immunology, 164:4991. The ED50 for this effect is typically 2-12 μg/mL.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM‑1 Monoclonal Antibody (Catalog # MAB1187) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
TREM‑1 in Mouse Splenocytes.
TREM‑1 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse TREM‑1 Monoclonal Antibody (Catalog # MAB1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.
Bouchon, A. (2000) J. Immunol. 164:4991.
Bouchon, A. (2001) Nature 410:1103.
Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs:
54210 (Human); 58217 (Mouse)
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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