Mouse TREM-1 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 174031
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
    Ala21-Ser202
    Accession # Q9JKE2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse TREM‑1 Fc Chimera (Catalog # 1187-TR)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Agonist Activity
    Measured by its ability to stimulate TNF-alpha secretion by P388D1 mouse lymphoma cells (ATCC # TIB-63). Bouchon, A. et al. (2001) Nature, 410:1103 and Bouchon, A. et al. (2000) J. Immunology, 164:4991.
    The ED50 for this effect is typically 2-12 μg/mL.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody
(Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM‑1 Monoclonal Antibody (Catalog # MAB1187) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). 
Immunocytochemistry
TREM‑1 in Mouse Splenocytes. TREM‑1 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse TREM‑1 Monoclonal Antibody (Catalog # MAB1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

  • References:
    1. Bouchon, A. (2000) J. Immunol. 164:4991.
    2. Bouchon, A. (2001) Nature 410:1103.
    3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells 1
  • Entrez Gene IDs:
    54210 (Human); 58217 (Mouse)
  • Alternate Names:
    CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
    Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
    Sci Rep, 2017;7(0):42028.
  2. TREM-2 promotes macrophage survival and lung disease after respiratory viral infection.
    Authors: Wu K, Byers D, Jin X, Agapov E, Alexander-Brett J, Patel A, Cella M, Gilfilan S, Colonna M, Kober D, Brett T, Holtzman M
    J Exp Med, 2015;212(5):681-97.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
  3. TREM-1 deficiency can attenuate disease severity without affecting pathogen clearance.
    Authors: Weber B, Schuster S, Zysset D, Rihs S, Dickgreber N, Schurch C, Riether C, Siegrist M, Schneider C, Pawelski H, Gurzeler U, Ziltener P, Genitsch V, Tacchini-Cottier F, Ochsenbein A, Hofstetter W, Kopf M, Kaufmann T, Oxenius A, Reith W, Saurer L, Mueller C
    PLoS Pathog, 2014;10(1):e1003900.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  4. Comparison of gene expression profiles between human and mouse monocyte subsets.
    Authors: Ingersoll MA, Spanbroek R, Lottaz C
    Blood, 2010;115(3):e10-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. TREM-1 activation alters the dynamics of pulmonary IRAK-M expression in vivo and improves host defense during pneumococcal pneumonia.
    Authors: Lagler H, Sharif O, Haslinger I, Matt U, Stich K, Furtner T, Doninger B, Schmid K, Gattringer R, de Vos AF, Knapp S
    J. Immunol., 2009;183(3):2027-36.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
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