Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, Agonist Activity, CyTOF-ready

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Capture, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 174031
View all TREM-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
Ala21-Ser202
Accession # Q9JKE2

Specificity

Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse TREM‑1 Antibody

Detection of TREM-1 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
TREM‑1 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

TREM‑1 in Mouse Splenocytes.

TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of TREM-1 by Immunocytochemistry/ Immunofluorescence

Detection of TREM-1 by Immunocytochemistry/ Immunofluorescence

ExNef potentiate inflammatory signalling cascade via re-localization of TLR4 & TREM-1 to lipid rafts.A–The effect of exNef (0.4 ng/ml, 48h) on abundance of lipid rafts & re-localization of TLR4 & TREM-1 to the plasma membrane in RAW264.7 macrophages. Left column, CTB staining; second column, anti-TREM-1 staining; third column anti-TLR4 staining; fourth column–merge TREM-1/rafts, right column–merge TLR4/rafts. Scale bars 10 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31344124), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TREM-1 by Immunocytochemistry/ Immunofluorescence

Detection of TREM-1 by Immunocytochemistry/ Immunofluorescence

TREM-1 activation induced HIF-1 alpha accumulation and translocation to the nucleus in macrophages. Macrophages were incubated with anti-TREM-1 mAb (10 μg/mL) in a normoxia condition. (A) Six hours later, Hif‐1 alpha gene levels were measured using qPCR, n=4-6. (B-C) Twenty-four hours later, the protein of HIF‐1 alpha was performed by western blot with GAPDH as a loading control, n=3. (D) Macrophages were cultured on anti-TREM-1 for 24 h and then subjected to immunofluorescence examination to analyze the HIF-1 alpha accumulation and translocation to the nucleus (scale bar, 50 μm). (E) Average fluorescent intensity was calculated by HIF-1 alpha + fluorescence intensity (IntDen)/area of the region (Area) using ImageJ, n=6. (F-G) 1×106 cells/well were premixed with PBS control or PX-478 (25 μM) for 30 min, then plated into 12-well plates with agonist anti-TREM-1 mAb (10 μg/mL). HK2 protein levels were measured after an additional incubation for 24 h, n=3. n represents experiments performed on different macrophages from separate mice. Data are expressed as the mean ± SD. Student's t-test (two-tailed, unpaired) was used to compare Mab1187 and Control in (A-E): * P < 0.05, ** P < 0.01, and *** P < 0.001. One-way ANOVA adjusted by Tukey's multiple comparison test was used in G: *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36594089), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TREM-1 by Immunocytochemistry/ Immunofluorescence

Detection of TREM-1 by Immunocytochemistry/ Immunofluorescence

TREM-1 activation induced HIF-1 alpha accumulation and translocation to the nucleus in macrophages. Macrophages were incubated with anti-TREM-1 mAb (10 μg/mL) in a normoxia condition. (A) Six hours later, Hif‐1 alpha gene levels were measured using qPCR, n=4-6. (B-C) Twenty-four hours later, the protein of HIF‐1 alpha was performed by western blot with GAPDH as a loading control, n=3. (D) Macrophages were cultured on anti-TREM-1 for 24 h and then subjected to immunofluorescence examination to analyze the HIF-1 alpha accumulation and translocation to the nucleus (scale bar, 50 μm). (E) Average fluorescent intensity was calculated by HIF-1 alpha + fluorescence intensity (IntDen)/area of the region (Area) using ImageJ, n=6. (F-G) 1×106 cells/well were premixed with PBS control or PX-478 (25 μM) for 30 min, then plated into 12-well plates with agonist anti-TREM-1 mAb (10 μg/mL). HK2 protein levels were measured after an additional incubation for 24 h, n=3. n represents experiments performed on different macrophages from separate mice. Data are expressed as the mean ± SD. Student's t-test (two-tailed, unpaired) was used to compare Mab1187 and Control in (A-E): * P < 0.05, ** P < 0.01, and *** P < 0.001. One-way ANOVA adjusted by Tukey's multiple comparison test was used in G: *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36594089), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TREM‑1 Antibody

Application
Recommended Usage

Agonist Activity

Measured by its ability to stimulate TNF-alpha secretion by P388D1 mouse lymphoma cells (ATCC # TIB-63). Bouchon, A. et al. (2001) Nature, 410:1103 and Bouchon, A. et al. (2000) J. Immunology, 164:4991.
The ED50 for this effect is typically 0.4-3 μg/mL.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes treated with LPS

Western Blot

1 µg/mL
Sample: Recombinant Mouse TREM‑1 Fc Chimera (Catalog # 1187-TR)

Reviewed Applications

Read 1 review rated 4 using MAB1187 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References

  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9JKE2

Additional TREM-1 Products

Product Documents for Mouse TREM‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TREM‑1 Antibody

For research use only

Citations for Mouse TREM‑1 Antibody

Customer Reviews for Mouse TREM‑1 Antibody (1)

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  • Mouse TREM-1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 08/28/2022
    Works well with flow!
    Mouse TREM‑1 Antibody MAB1187

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Protocols

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