Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry, In vivo assay, Neutralizing

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 152614
View all TSLP Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6

Specificity

Detects mouse TSLP in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse TSLP Antibody

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.

Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND50 is typically 0.1-0.4 µg/mL.
Detection of Mouse TSLP by Block/Neutralize

Detection of Mouse TSLP by Block/Neutralize

TSLP neutralization inhibits airway structural alterations.(A) The depth of peribronchial collagen deposition was significantly reduced in anti-TSLP-treated mice, compared with HDM-exposed and IgG isotype-treated controls. (B) Epithelial goblet cells were markedly reduced in anti-TSLP-treated mice, compared to the HDM-exposed and IgG -treated controls. (C) TGF-beta 1 levels in the BALF were significantly increased in HDM-exposed mice. The blockage of TSLP with the anti-TSLP mAb reduced TGF-beta 1levels in the BALF. TGF-beta 1 protein and mRNA levels were determined in duplicate experiments. (D-G) Representative photomicrographs of Masson's trichrome-stained lung sections from mice that were exposed for 5 weeks to saline (D) or HDM (E) and pretreated with anti-TSLP mAb (F) or an IgG-treated control (G) 60 minutes prior to and during HDM exposure. (H-K) Representative photomicrographs of airway sections of PAS-stained from mice that were exposed for 5 weeks to saline (H) or HDM (I) and pretreated with anti-TSLP mAb (J) or an IgG-treated control (K) 60 minutes prior to and during the HDM exposure. Original magnification, 20×. The data shown represent the means±SEM (n = 5), **p<0.01 compared with the HDM group, ##p<0.01 compared with the IgG-treated control mice. The photomicrographs of H-K are representative of 5 independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0051268), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse TSLP Antibody by Flow Cytometry

Detection of Mouse Mouse TSLP Antibody by Flow Cytometry

TSLP neutralization inhibits surface marker expression on airway CD11c+ cells.(A) Lung cell suspensions from mice were stained with combinations of PerCP-Cy 5.5-conjugated CD11c, PE-conjugated OX40L, FITC-conjugated CD80 and APC-conjugated CD86. The dead cells and the debris were excluded based on forward scatter (FSC)/side scatter (SSC) plots. (B) A total of 1×105 to 3×105 viable cells were acquired using FACS, and the DCs were then sorted as CD11c+ airway cells on FSC/SSC plots. (C) Mice that were chronically exposed to HDM received an intranasal administration of anti-TSLP mAb or of a control IgG 60 minutes prior to each HDM exposure. The expression levels of OX40L, CD80 and CD86 on pulmonary DCs were then analyzed using flow cytometry. The HDM-exposed mice exhibited a significant increase in the OX40L, CD80 and CD86 surface markers on CD11c+ airway cells. However, anti-TSLP pretreatment effectively reduced OX40L, CD80 and CD86 expression on the DCs, even though the mice were continuously exposed to HDM. (D) The TSLP levels in the BALF were highly correlated with OX40L expression on CD11c+ airway cells in all of the treatment mice (n = 20, R = 0.879, p<0.01). (E) Representative data from one of the five replicates. (F) Staining for OX40L (12-4031), CD80 (11-4888), and CD86 (17-4321) from isotype-Ig-treated controls. The data represent the means±SEM (n = 5). * p<0.05 or ** p<0.01 compared with the HDM group. # p<0.05 or ## p<0.01 compared with the IgG-treated control mice. The results are derived from 4 experimental groups (5 mice/per group), and the data are representative of 5 independent experiment. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23300949), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TSLP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TSLP (Catalog # 555-TS)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha. Park, L.S. et al. (2000) J. Exp. Med. 192:659. The Neutralization Dose (ND50) is typically 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.

Reviewed Applications

Read 2 reviews rated 3.5 using MAB555 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSLP

TSLP is an IL-7 related cytokine secreted by multiple tissues. It interacts with the heterodimeric receptor complex consisting of IL-7 R alpha and TSLP R. Mouse TSLP stimulates the growth of thymocytes and mature T cells and also supports B cell lymphopoiesis.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

UniProt

Q9JIE6

Additional TSLP Products

Product Documents for Mouse TSLP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TSLP Antibody

For research use only

Citations for Mouse TSLP Antibody

Customer Reviews for Mouse TSLP Antibody (2)

3.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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2 Stars
50%
1 Stars
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Showing  1 - 2 of 2 reviews Showing All
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  • Mouse TSLP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 09/27/2021
    Mouse TSLP Antibody MAB555
  • Mouse TSLP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 02/26/2021
    Mouse TSLP Antibody MAB555
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. Although MAB555 has not been validated by Bio-Techne for IHC/ICC/IF application, Technical Service provided troubleshooting suggestions, which resulted in a weak signal in tissues tested. Customer observed a good signal with polyclonal antibody from Novus, catalog # NB110-55234.

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