Mouse TSLP Antibody

(13 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TSLP in Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 152614
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
    Tyr20-Glu140
    Accession # Q9JIE6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse TSLP (Catalog # 555-TS)
    under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha. Park, L.S. et al. (2000) J. Exp. Med. 192:659. The Neutralization Dose (ND50) is typically 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLP
TSLP is an IL-7 related cytokine secreted by multiple tissues. It interacts with the heterodimeric receptor complex consisting of IL-7 R alpha and TSLP R. Mouse TSLP stimulates the growth of thymocytes and mature T cells and also supports B cell lymphopoiesis.
  • Long Name:
    Thymic Stromal Lymphopoietin
  • Entrez Gene IDs:
    85480 (Human); 53603 (Mouse)
  • Alternate Names:
    thymic stromal lymphopoietin; TSLP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Frontline Science: Nasal epithelial GM-CSF contributes to TLR5-mediated modulation of airway dendritic cells and subsequent IgA response
    Authors: Y Cao, E Zhang, J Yang, Y Yang, J Yu, Y Xiao, W Li, D Zhou, Y Li, B Zhao, H Yan, M Lu, M Zhong, H Yan
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Thymic Stromal Lymphopoietin Neutralization Inhibits the Immune Adjuvant Effect of Di-(2-Ethylhexyl) Phthalate in Balb/c Mouse Asthma Model
    PLoS ONE, 2016;11(7):e0159479.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. Critical role of TSLP-responsive mucosal dendritic cells in the induction of nasal antigen-specific IgA response
    Mucosal Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Conditional PDK1 Ablation Promotes Epidermal and T-Cell-Mediated Dysfunctions Leading to Inflammatory Skin Disease.
    Authors: Yu M, Owens D, Ghosh S, Farber D
    J Invest Dermatol, 2015;135(11):2688-96.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  5. Epidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility.
    Authors: Cipolat S, Hoste E, Natsuga K, Quist S, Watt F
    Elife, 2014;3(0):e01888.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  6. Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
    Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K
    J Invest Dermatol, 2014;134(5):1295-304.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  7. Peroxisome proliferator-activated receptor gamma-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/Tnd mice.
    Authors: Jung K, Tanaka A, Fujita H, Matsuda A, Oida K, Karasawa K, Okamoto N, Ohmori K, Jee Y, Shin T, Matsuda H
    J. Allergy Clin. Immunol., 2011;127(2):420-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  8. Thymic stromal lymphopoietin is a key mediator of breast cancer progression.
    Authors: Olkhanud PB, Rochman Y, Bodogai M
    J. Immunol., 2011;186(10):5656-62.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  9. IL-13 induces skin fibrosis in atopic dermatitis by thymic stromal lymphopoietin.
    Authors: Oh MH, Oh SY, Yu J
    J. Immunol., 2011;186(12):7232-42.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. A small molecule CRTH2 antagonist inhibits FITC-induced allergic cutaneous inflammation.
    Authors: Boehme SA, Franz-Bacon K, Chen EP, Sasik R, Sprague LJ, Ly TW, Hardiman G, Bacon KB
    Int. Immunol., 2009;21(1):81-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  11. Thymic stromal lymphopoietin is a critical mediator of IL-13-driven allergic inflammation.
    Authors: Miyata M, Nakamura Y, Shimokawa N, Ohnuma Y, Katoh R, Matsuoka S, Okumura K, Ogawa H, Masuyama K, Nakao A
    Eur. J. Immunol., 2009;39(11):3078-83.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. A mechanism for the initiation of allergen-induced T helper type 2 responses.
    Authors: Sokol CL, Barton GM, Farr AG, Medzhitov R
    Nat. Immunol., 2008;9(3):310-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007;109(9):3706-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 13 Citations
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