Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP Tyr20-Glu140 Accession # Q9JIE6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse TSLP (Catalog # 555-TS) under non-reducing conditions only
Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha. Park, L.S. et al. (2000) J. Exp. Med. 192:659. The Neutralization Dose (ND50) is typically 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TSLP is an IL-7 related cytokine secreted by multiple tissues. It
interacts with the heterodimeric receptor complex consisting of IL-7 R alpha
and TSLP R. Mouse TSLP stimulates the growth of thymocytes and mature T
cells and also supports B cell lymphopoiesis.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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