Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 152640
View all TSLP Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6

Specificity

Detects mouse TSLP in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑7 or recombinant human TSLP.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse TSLP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TSLP (Catalog # 555-TS)
under non-reducing conditions only

Mouse TSLP Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse TSLP Biotinylated Antibody (Catalog # BAF555)
  • Standard: Recombinant Mouse TSLP Protein (Catalog # 555-TS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSLP

TSLP is an IL-7 related cytokine secreted by multiple tissues. It interacts with the heterodimeric receptor complex consisting of IL-7 R alpha and TSLP R. Mouse TSLP stimulates the growth of thymocytes and mature T cells and also supports B cell lymphopoiesis.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

UniProt

Q9JIE6

Additional TSLP Products

Product Documents for Mouse TSLP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TSLP Antibody

For research use only

Citations for Mouse TSLP Antibody

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FAQs

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