Mouse TSLP Antibody (152640)

R&D Systems | Catalog # MAB5551

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 152640
View all TSLP Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6

Specificity

Detects mouse TSLP in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑7 or recombinant human TSLP.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse TSLP Antibody (152640)

Mouse TSLP ELISA Standard Curve

Mouse TSLP ELISA Standard Curve

Recombinant Mouse TSLP (Catalog # 555-TS) was serially diluted and captured by Rat Anti-Mouse TSLP Monoclonal Antibody (Catalog # MAB5551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse TSLP Antibody (152640)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TSLP (Catalog # 555-TS)
under non-reducing conditions only

Mouse TSLP Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse TSLP Biotinylated Antibody (Catalog # BAF555)
  • Standard: Recombinant Mouse TSLP Protein (Catalog # 555-TS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSLP

TSLP is an IL-7 related cytokine secreted by multiple tissues. It interacts with the heterodimeric receptor complex consisting of IL-7 R alpha and TSLP R. Mouse TSLP stimulates the growth of thymocytes and mature T cells and also supports B cell lymphopoiesis.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

UniProt

Q9JIE6

Additional TSLP Products

Product Documents for Mouse TSLP Antibody (152640)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TSLP Antibody (152640)

For research use only

Citations for Mouse TSLP Antibody (152640)

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