Mouse VEGF R2/KDR/Flk-1 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse VEGF R2 in Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 91202
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2
    Ala20-Glu762
    Accession # P35918
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse VEGF R2/Flk-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse VEGF R2/KDR/Flk‑1 Antibody (Catalog # MAB4431)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse VEGF R2/KDR/Flk‑1 Biotinylated Antibody (Catalog # BAF644)
  • ELISA Standard
     
    Recombinant Mouse VEGF R2/Flk-1 Fc Chimera Protein (Catalog # 443-KD)
  • Neutralization
    Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 150 ng/mL Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Mouse VEGF164.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
VEGF R2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGF R2/KDR/Flk‑1 Antibody. Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164 (Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164 (5 ng/mL) activity elicited by Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (150 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGF R2/KDR/Flk‑1 Monoclonal Antibody (Catalog # MAB4431). The ND50 is typically 0.05-0.15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R2/KDR/Flk-1

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGF R2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  • References:
    1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
    2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  • Long Name:
    Vascular Endothelial Growth Factor Receptor 2
  • Entrez Gene IDs:
    3791 (Human); 16542 (Mouse)
  • Alternate Names:
    CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Mycobacterium tuberculosis exploits the formation of new blood vessels for its dissemination
    Sci Rep, 2016;6(0):33162.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors.
    Authors: Voron T, Colussi O, Marcheteau E, Pernot S, Nizard M, Pointet A, Latreche S, Bergaya S, Benhamouda N, Tanchot C, Stockmann C, Combe P, Berger A, Zinzindohoue F, Yagita H, Tartour E, Taieb J, Terme M
    J Exp Med, 2015;212(2):139-48.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis and late-stage focal edema, neural cell loss, and dysfunction.
    Authors: Nagai, Norihiro, Lundh von Leithner, Pete, Izumi-Nagai, Kanako, Hosking, Brett, Chang, Bo, Hurd, Ron, Adamson, Peter, Adamis, Anthony, Foxton, Richard, Ng, Yin Shan, Shima, David T
    Invest Ophthalmol Vis Sci, 2014;55(6):3709-19.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  4. alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner.
    Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM
    Blood, 2007;111(4):1980-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
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Rat IgG2A Isotype Control

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Normal Rat IgG Control

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Rat IgG2A Isotype Control

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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