Mouse VEGFR2/KDR/Flk-1 Antibody

Name: Mouse VEGFR2/KDR/Flk-1 Antibody
Brand: R&D Systems
SKU: MAB4431
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB4431

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 91202

View all VEGFR2/KDR/Flk-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2
Ala20-Glu762
Accession # P35918

Specificity

Detects mouse VEGFR2 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse VEGFR2/KDR/Flk-1 Antibody

VEGFR2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGFR2/KDR/Flk‑1 Antibody.

Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF₁₆₄(Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF₁₆₄(5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (150 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB4431). The ND₅₀ is typically 0.05-0.15 µg/mL.

Applications for Mouse VEGFR2/KDR/Flk-1 Antibody

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize VEGFR2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.05-0.15 µg/mL in the presence of 150 ng/mL Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Mouse VEGF₁₆₄.

Mouse VEGFR2/Flk-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse VEGFR2/KDR/Flk-1 Biotinylated Antibody (Catalog # BAF644)
Standard: Recombinant Mouse VEGFR2/Flk-1 Fc Chimera Protein (Catalog # 443-KD)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGFR2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.