NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34518

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Imaging Mass Cytometry

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 123A8 (same as 56C04)

Format

Azide and BSA Free
View all NCAM-1/CD56 Primary Antibodies »

Product Specifications

Immunogen

Membrane preparation of a small cell lung carcinoma

Localization

Cell surface

Marker

Neuronal Cell Marker

Specificity

This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34279).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

Western Blot: NCAM-1/CD56 Antibody (123A8 (same as 56C04))Azide and BSA Free [NBP2-34518]

Western Blot: NCAM-1/CD56 Antibody (123A8 (same as 56C04))Azide and BSA Free [NBP2-34518]

Western Blot: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Western blot analysis of human brain tissue lysate using NCAM-1/CD56 antibody (123A8 (same as 56C04)).
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Formalin-fixed, paraffin-embedded human colon stained with NCAM-1/CD56 Antibody (123A8 (same as 56C04)).
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Formalin-fixed, paraffin-embedded human Pancreas stained with NCAM-1/CD56 Antibody (123A8 (same as 56C04))
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Formalin-fixed, paraffin-embedded human thyroid stained with NCAM-1/CD56 Antibody (123A8 (same as 56C04)). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Imaging Mass Cytometry: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Imaging Mass Cytometry: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518]

Imaging Mass Cytometry: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Image of human cutaneous squamous cell carcinoma tumor stained with Anti-NCAM-1/CD56 Azide/BSA Free Antibody [123A8] (in red). IMC image submitted by a verified customer review.
NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

NCAM-1/CD56 Antibody (123A8 (same as 56C04))

Formalin-fixed, paraffin-embedded human thyroid stained with NCAM-1/CD56 antibody (123A8 (same as 56C04)). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-34518 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: NCAM-1/CD56

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Gene Symbol

NCAM1

Additional NCAM-1/CD56 Products

Product Documents for NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

Customer Reviews for NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free (1)

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Customer Images

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  • NCAM-1/CD56 Antibody (123A8 (same as 56C04))
    Name: Aida Meghraoui
    Application: Imaging Mass Cytometry
    Sample Tested: FFPE
    Species: Human
    Verified Customer | Posted 10/05/2020
    Mass Cytometry - Image of human cutaneous squamous cell carcinoma tumor stained with Anti-NCAM-1/CD56 Azide/BSA Free Antibody [123A8] (in red).
    NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free NBP2-34518

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