NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-33132
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]](https://resources.rndsystems.com/images/products/NCAM-1-CD56-Antibody-123C3-D5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33132-img0002.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 123C3.D5
Format
Azide and BSA Free
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Product Specifications
Immunogen
Membrane preparation of a small cell lung carcinoma
Localization
Cell surface
Marker
Neuronal Cell Marker
Specificity
This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide.
Antibody without azide - store at -20 to -80C.
Antibody without azide - store at -20 to -80C.
Scientific Data Images for NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Formalin-fixed paraffin-embedded human colon Ganglion stained with CD56 Monoclonal antibody (123C3.D5)![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]](https://resources.rndsystems.com/images/products/NCAM-1-CD56-Antibody-123C3-D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33132-img0008.jpg "Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]")
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]](https://resources.rndsystems.com/images/products/NCAM-1-CD56-Antibody-123C3-D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33132-img0001.jpg "Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]")
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132]
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Analysis of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.![Simple Western: NCAM-1/CD56 Antibody (123C3.D5)Azide and BSA Free [NBP2-33132]](https://resources.rndsystems.com/images/products/NCAM-1-CD56-Antibody-123C3-D5-Azide-and-BSA-Free-Simple-Western-NBP2-33132-img0006.jpg "Simple Western: NCAM-1/CD56 Antibody (123C3.D5)Azide and BSA Free [NBP2-33132]")
Simple Western: NCAM-1/CD56 Antibody (123C3.D5)Azide and BSA Free [NBP2-33132]
Simple Western: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: NCAM-1/CD56 Antibody (123C3.D5)Azide and BSA Free [NBP2-33132]](https://resources.rndsystems.com/images/products/NCAM-1-CD56-Antibody-123C3-D5-Azide-and-BSA-Free-Simple-Western-NBP2-33132-img0007.jpg "Simple Western: NCAM-1/CD56 Antibody (123C3.D5)Azide and BSA Free [NBP2-33132]")
Simple Western: NCAM-1/CD56 Antibody (123C3.D5)Azide and BSA Free [NBP2-33132]
Simple Western: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.![NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free Western Blot: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] -](https://resources.rndsystems.com/images/products/nbp2-33132_mouse-monoclonal-ncam-1-cd56-antibody-123c3-d5-azide-and-bsa-free-262025168535.jpeg "Western Blot: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] -")
Western Blot: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] -
Western blot analysis of Human Brain tissue lysates using NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free.Applications for NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free
Application
Recommended Usage
Flow (Cell Surface)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: NCAM-1/CD56
Long Name
Neural Cell Adhesion Molecule
Alternate Names
CD56, NCAM1
Gene Symbol
NCAM1
Additional NCAM-1/CD56 Products
Product Documents for NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
