NCAM-1/CD56 Antibody (123C3.D5)

Novus Biologicals | Catalog # NBP2-15184

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 123C3.D5
View all NCAM-1/CD56 Primary Antibodies »

Product Specifications

Immunogen

Membrane preparation of a small cell lung carcinoma

Localization

Cell surface

Marker

Neuronal Cell Marker

Specificity

This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33132)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NCAM-1/CD56 Antibody (123C3.D5)

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis using the Azide and BSA Free version of NBP2-15184. Staining of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.
Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184]

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.
NCAM-1/CD56 Antibody (123C3.D5) Western Blot: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] -

Western Blot: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] -

Western blot analysis of Human Brain tissue lysates using NCAM-1/CD56 Antibody (123C3.D5).

Applications for NCAM-1/CD56 Antibody (123C3.D5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml
Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in IMR-32 lysate(s), separated by Size, antibody dilution of 10 ug/mL, apparent MW was 183 kDa.

Reviewed Applications

Read 2 reviews rated 4 using NBP2-15184 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NCAM-1/CD56

CD56, a 175-220KDa glycoprotein, is a member of the Ig super family. It is expressed as three major isoforms and consists of five Ig-like domains and two Fibronectin-type III domains in the extracellular region. The 135kDa isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. CD56 is widely expressed in nervous system, on NK cells and a specific set of Tcells. CD56+ NK cells and Tcells are unique in their ability to mediate cell-mediated cytotoxicity against certain tumor cell targets without MHC restriction. Other physiological functions of CD56 include mediating cell adhesion through homophilic and heterophilic interaction and activating intracellular signaling pathways resulting in neutrite extension and fasciculation, migration and synapses formation in brain. CD56 is also vital for neuronal development and plasticity in adult brain. It is used as a tumor marker in various cancers such as NK lymphomas and Merkel cell carcinoma. NCAM is expressed on most neuroectodermal derived lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Gene Symbol

NCAM1

UniProt

P13592 (Human), P13591 (Rat)

Additional NCAM-1/CD56 Products

Product Documents for NCAM-1/CD56 Antibody (123C3.D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NCAM-1/CD56 Antibody (123C3.D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NCAM-1/CD56 Antibody (123C3.D5)

Customer Reviews for NCAM-1/CD56 Antibody (123C3.D5) (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • NCAM-1/CD56 Antibody (123C3.D5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 02/12/2022
    Human colon tissue
    Incubation: 1 ug/ml for 30 minutes at room temperature.
    NCAM-1/CD56 Antibody (123C3.D5) NBP2-15184
  • NCAM-1/CD56 Antibody (123C3 (same as 123C3.D5))
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Cortical neurons
    Species: Mouse
    Verified Customer | Posted 09/21/2017

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