New Slit and ROBO Recombinant Proteins

Slits and ROBO receptors are perhaps best known for roles in axon guidance, but they have also been implicated in morphogenesis, tumorigenesis, cell/cell junction regulation, and migration.

Slit proteins contain four domains of leucine-rich repeats, seven to nine EGF-like sequences, and a laminin-G domain. In mammals, there are three slit genes, all of which are expressed in the nervous system and in other organs. Slit2 and Slit3 can be cleaved to yield a short C-terminal fragment that signals via PlexinA1 receptor, and a long N-terminal fragment that signals via ROBO receptors. R&D Systems offers the different forms.

The archetypal ROBO1-4 receptors possess five Ig-like domains, three fibronectin type III motifs, a transmembrane spanning region and four c-terminal domains. ROBO receptors can be alternatively spliced to generate various isoforms.

Features

• All Slit and ROBO proteins are bioactive, exclusive to R&D Systems
• Multiple variants are available

Recombinant Human C-Terminal Slit3 (aa 1120-1523) Induces Cortical Neurite Outgrowth of E18 Rat Cortical Neurons. A) Untreated E16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 1.25 µg/ml of Recombinant Human Slit3 (Catalog # 9067-SL).

Recombinant Human Slit2 Induces Cortical Neurite Outgrowth of E18 Rat Cortical Neurons. A) Untreated E16-18 embryonic rat cortical neurons. B) Recombinant Human Slit2 (Catalog # 8616-SL) immobilized at 2.5 µg/mL induces cortical neurite outgrowth of E18 embryonic rat cortical neurons.

Recombinant Human Slit3 (aa 34-1116) Binds to Recombinant Human ROBO1/DUTT1 in ELISA Binding Assays. When recombinant human Slit3 is coated at 250 ng/mL, recombinant human ROBO1 Fc Chimera (Catalog # 8975-RB) binds with an ED⁵⁰ of 100-600 ng/ml.

Recombinant Human ROBO4 Binds to Recombinant Rat UNC5H2 in ELISA Binding Assays. When recombinant human ROBO4 Fc Chimera (Catalog # 2366-RB) is immobilized at 5 μg/mL, biotinylated recombinant rat UNC5H2/Fc Chimera binds with an apparent KD < 20 nM.

Recombinant Mouse Slit2 Supports Pancreatic Beta TC3 Cell Survival. Recombinant Mouse Slit2 (Catalog # 5444-SL) supports mouse pancreatic beta TC3 cell survival in the presence of 20 mM glucose. Cell number was measured by Resazurin (Catalog # AR002) reduction in viable cells.