Slits and ROBO receptors are perhaps best known for roles in axon guidance, but they have also been implicated in morphogenesis, tumorigenesis, cell/cell junction regulation, and migration.
Slit proteins contain four domains of leucine-rich repeats, seven to nine EGF-like sequences, and a laminin-G domain. In mammals, there are three slit genes, all of which are expressed in the nervous system and in other organs. Slit2 and Slit3 can be cleaved to yield a short C-terminal fragment that signals via PlexinA1 receptor, and a long N-terminal fragment that signals via ROBO receptors. R&D Systems offers the different forms.
The archetypal ROBO1-4 receptors possess five Ig-like domains, three fibronectin type III motifs, a transmembrane spanning region and four c-terminal domains. ROBO receptors can be alternatively spliced to generate various isoforms.
|Recombinant Protein||Species||Catalog Number|
|Slit Proteins (amino acids)|
|Slit1 (aa 1-915)||human||6514-SL|
|Slit1 (aa 34-900)||mouse||5199-SL|
|Slit2 (aa 26-1118)||human||8616-SL|
|Slit2 (aa 1122-1529) NEW||human||9379-SL|
|Slit2 (aa 26-900)||mouse||5444-SL|
|Slit2 (aa 1114-1521) NEW||mouse||9430-SL|
|Slit3 (aa 1120-1523)||human||9067-SL|
|Slit3 (aa 34-1116) NEW||human||9255-SL|
|Slit3 (aa 34-1116)||mouse||9296-SL|
|SLIT-3 (aa 1117-1523) NEW||mouse||9295-SL|
|Robo Proteins (amino acids)|
|ROBO1/DUTT1 (aa 20-858) NEW||human||8975-RB|
|ROBO1 (aa 19-560)||rat||1749-RB|
|ROBO2 (aa 22-859)||human||3147-RB|
|ROBO2 (aa 22-863) NEW||mouse||8366-RB|
|ROBO3 (aa 40-545)||human||3076-RB|
|ROBO3 (aa 54-545)||mouse||3155-RB|
|ROBO4 (aa 28-467)||human||2366-RB|
Recombinant Human Slit3 (aa 34-1116) Binds to Recombinant Human ROBO1/DUTT1 in ELISA Binding Assays. When recombinant human Slit3 is coated at 250 ng/mL, recombinant human ROBO1 Fc Chimera (Catalog # 8975-RB) binds with an ED50 of 100-600 ng/ml.
Recombinant Human ROBO4 Binds to Recombinant Rat UNC5H2 in ELISA Binding Assays. When recombinant human ROBO4 Fc Chimera (Catalog # 2366-RB) is immobilized at 5 μg/mL, biotinylated recombinant rat UNC5H2/Fc Chimera binds with an apparent KD < 20 nM.
Recombinant Mouse Slit2 Supports Pancreatic Beta TC3 Cell Survival. Recombinant Mouse Slit2 (Catalog # 5444-SL) supports mouse pancreatic beta TC3 cell survival in the presence of 20 mM glucose. Cell number was measured by Resazurin (Catalog # AR002) reduction in viable cells.