Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-62557

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Citations (1)

Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # E6

Format

Azide and BSA Free

View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was selected against a fusion protein consisting of EGF domains 1-12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1.

Specificity

An engineered ScFv fragment antibody that binds to both mouse mNRR1 and human hNRR1 but not NRR2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Description

Novus Biologicals Rabbit Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free (NBP2-62557) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. Anti-Notch-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free

Flow Cytometry: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557]

Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunocytochemistry /Immunofluorescence: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557] -

Immunocytochemistry /Immunofluorescence: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-62557 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62557, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free

Application

Recommended Usage

ELISA

1:100 - 1:1000

Immunohistochemistry

1:10 - 1:500

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original human scFv format, for improved compatibility with existing reagents, assays and techniques.

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free

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Product Specific Notices for Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.