p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free

Name: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-73209
Price: 559 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-73209

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI1G4

Format

Azide and BSA Free

View all p70 S6 Kinase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RPS6KB1(NP_003152) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free (NBP2-00754) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free

Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4)Azide and BSA Free [NBP2-73209]

Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S6K (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S6K.

Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209]

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY S6K.

Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209]

Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-S6K mouse monoclonal antibody.

Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209]

Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-S6K antibody, and then analyzed by flow cytometry.

Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4)Azide and BSA Free [NBP2-73209]

Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.

Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209]

Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-S6K mouse monoclonal antibody.

Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209]

Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of paraffin-embedded Human pancreas tissue using anti-S6K mouse monoclonal antibody.

Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209]

Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Analysis of Jurkat cells, using anti-S6K antibody, (Red), compared to a nonspecific negative control antibody (Blue).

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Applications for p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: p70 S6 Kinase

S6K encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Gene Symbol

RPS6KB1

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.