PDCD4 [p Ser457] Antibody - BSA Free

Novus Biologicals | Catalog # NB110-60014

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDCD4 Primary Antibodies »

Product Specifications

Immunogen

This affinity purified PDCD4 [p Ser457] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human PDCD4 protein. (Uniprot: Q53EL6)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with PDCD4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with PDCD4 from other sources has not been determined.

Modification

p Ser457

Specificity

This antibody is specific for human PDCD4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with PDCD4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with PDCD4 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for PDCD4 [p Ser457] Antibody - BSA Free

Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014]

Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014]

Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014] - Analysis of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteasome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).
Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014]

Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014]

Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014] - Used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
PDCD4 [p Ser457] Antibody

PDCD4 [p Ser457] Antibody

affinity purified anti-Pdcd4 pS457 antibody was used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
PDCD4 [p Ser457] Antibody

PDCD4 [p Ser457] Antibody

Western blot using affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (lanes 1 & 3) at ~52kDa (arrow). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4). Personal Communication, M Young & A Jansen, NCI, Bethesda, MD.

Applications for PDCD4 [p Ser457] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:400000

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

21.25-2.5 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 52 kDa in size corresponding to Pdcd4 protein is expected in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: PDCD4

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun.

Long Name

Programmed Cell Death 4

Alternate Names

H731, Protein 197/15a

Entrez Gene IDs

27250 (Human); 64031 (Rat)

Gene Symbol

PDCD4

UniProt

Q53EL6 (Human)

Additional PDCD4 Products

Product Documents for PDCD4 [p Ser457] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDCD4 [p Ser457] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Translation Regulation

Citations for PDCD4 [p Ser457] Antibody - BSA Free

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Protocols

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