PDK-1 Antibody (M146)

Name: PDK-1 Antibody (M146)
Brand: Novus Biologicals
SKU: NBP3-23291
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23291

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # M146

View all PDK-1 Primary Antibodies »

Product Specifications

Immunogen

Clone M146 was generated from a recombinant protein containing amino acid residues in the C-terminal region of human PDK-1. This sequence is highly conserved in rat and mouse PDK-1. [UniProt# O15530]

Specificity

The antibody detects a 65 kDa protein corresponding to the apparent molecular mass of PDK-1 on SDS-PAGE immunoblots of human A431 cells and mouse brain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PDK-1 Antibody (M146)

Immunocytochemistry/Immunofluorescence: PDK-1 Antibody (M146) [NBP3-23291] -

Immunocytochemistry/Immunofluorescence: PDK-1 Antibody (M146) [NBP3-23291] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK-1 (C-terminal Region) (NBP3-23291), rabbit polyclonal PDK-1 (Tyr-9) (NBP3-23292), and PDK-1 (N-terminus) (NBP3-23290) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Western Blot: PDK-1 Antibody (M146) [NBP3-23291] -

Western Blot: PDK-1 Antibody (M146) [NBP3-23291] - Western blot image of mouse brain (lane 1) and human A431 cells (lane 2). The blot was probed with anti-PDK-1 (C-terminal region) (NBP3-23291).

Applications for PDK-1 Antibody (M146)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:250

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PDK-1

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Gene Symbol

PDPK1

Additional PDK-1 Products

Product Documents for PDK-1 Antibody (M146)

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Certificate of Analysis

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Product Specific Notices for PDK-1 Antibody (M146)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.