Phospho-ERK2 (T185/Y187) DuoSet IC ELISA

Discontinued Product

DYC1483-2 has been discontinued.
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Citations (5)
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Phospho-ERK2 (T185/Y187) DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
188.0 - 12,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat ERK2 (T185 / Y187) in cell lysates. An immobilized capture antibody specific for ERK2 binds both phosphorylated and unphosphorylated ERK2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*


Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Long Name:
Extracellular Signal-regulated Kinase 2
Entrez Gene IDs:
5594 (Human); 26413 (Mouse); 116590 (Rat)
Alternate Names:
EC 2.7.11; EC 2.7.11.24; ERK; ERK2; ERK-2; ERK2MAP kinase isoform p42; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK1; MAPK2; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM1MAPK 2; PRKM2; protein tyrosine kinase ERK2

Citations for Phospho-ERK2 (T185/Y187) DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Anti-Septic Functions of Cornuside against HMGB1-Mediated Severe Inflammatory Responses
    Authors: N Kim, C Kim, SH Ryu, W Lee, JS Bae
    International Journal of Molecular Sciences, 2022-02-13;23(4):.
    Species: Human
    Sample Types: Cell Lysates
  2. Real-time monitoring of Cisplatin-induced cell death.
    Authors: Alborzinia H, Can S, Holenya P, Scholl C, Lederer E, Kitanovic I, Wolfl S
    PLoS ONE, 2011-05-16;6(5):e19714.
    Species: Human
    Sample Types: Cell Lysates
  3. Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis.
    Authors: Holenya P, Kitanovic I, Heigwer F, Wolfl S
    Proteomics, 2011-04-18;11(10):2129-33.
    Species: Human
    Sample Types: Recombinant Protein
  4. A phase II clinical trial of sorafenib in androgen-independent prostate cancer.
    Authors: Dahut WL, Scripture C, Posadas E, Jain L, Gulley JL, Arlen PM, Wright JJ, Yu Y, Cao L, Steinberg SM, Aragon-Ching JB, Venitz J, Jones E, Chen CC, Figg WD
    Clin. Cancer Res., 2008-01-01;14(1):209-14.
    Species: Human
    Sample Types: Cell Lysates
  5. Proteolytic processing of CXCL11 by CD13/aminopeptidase N impairs CXCR3 and CXCR7 binding and signaling and reduces lymphocyte and endothelial cell migration.
    Authors: Proost P, Mortier A, Loos T, Vandercappellen J, Gouwy M, Ronsse I, Schutyser E, Put W, Parmentier M, Struyf S, Van Damme J
    Blood, 2007-03-15;110(1):37-44.
    Species: Hamster
    Sample Types: Cell Lysates

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