Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Multi-Species

Cited:

Insect - Drosophila

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 216954
View all Phospho-Tyrosine Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Phospho-Tyrosine synthetic peptide

Specificity

Detects proteins containing phosphorylated tyrosine residues in Western blots. In Western blots of pervanadate-treated cell lysates, clone 216954 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Phospho-Tyrosine Antibody

Detection of Phospho-Tyrosine antibody by Western Blot.

Detection of Phospho-Tyrosine by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB16761), followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Applications for Phospho-Tyrosine Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate

Western Blot

1 µg/mL
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB16761 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-Tyrosine Antibody

For research use only

Related Research Areas

GDNF Family

Citations for Phospho-Tyrosine Antibody

Customer Reviews for Phospho-Tyrosine Antibody (1)

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  • Phospho-Tyrosine Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: PC-12 cells
    Species: Rat
    Verified Customer | Posted 12/02/2021
    Phospho-Tyrosine Antibody MAB16761

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