PKC alpha Antibody (MC5) - BSA Free

A: We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.

A: Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.

A: According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

A: We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

A: We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.

A: Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.

A: According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

A: We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

A: We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.

A: Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.

A: According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

A: We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

A: We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.

A: Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.

A: According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

A: We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.