IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63% - 70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta.
Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody
R&D Systems | Catalog # BAF681
Key Product Details
Species Reactivity
Validated:
Porcine
Cited:
Human, Porcine, Boar
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Western Blot, Flow Cytometry, Detection Antibody, ELISA Detection, ELISA Development, Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant porcine IL‑1 beta /IL-1F2 (R&D Systems, Catalog # 681-PI)
Ala115-Pro267
Accession # P26889
Ala115-Pro267
Accession # P26889
Specificity
Detects porcine IL‑1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1.5% cross-reactivity with recombinant rat (rr) IL‑1 beta is observed and less than 0.4% cross-reactivity with recombinant mouse (rm) IL‑1 beta, recombinant cotton rat IL‑1 beta, and recombinant human (rh) IL‑1 beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI)
Sample: Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI)
Porcine IL-1 beta /IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 5 using BAF681 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-1 beta/IL-1F2
References
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Huether, M. et al. (1993) Gene 129:285.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
UniProt
Additional IL-1 beta/IL-1F2 Products
Product Documents for Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody
For research use only
Related Research Areas
Citations for Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody
Powered by Bioz
Customer Reviews for Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody (3)
5 out of 5
3 Customer Ratings
Have you used Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
3 of
3 reviews
Showing All
Filter By:
-
Application: ELISASample Tested: Cell culture supernatantSpecies: PorcineVerified Customer | Posted 11/17/2018
-
Application: Western BlotSample Tested: Cell LysatesSpecies: PorcineVerified Customer | Posted 04/25/2018
-
Application: ELISASample Tested: EDTA PlasmaSpecies: swineVerified Customer | Posted 09/07/2017
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
IL-1 Family Signaling Pathways
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Inflammasome Activation Pathways
Innate Lymphoid Cell Differentiation Pathways
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways