Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Porcine

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, Immunocytochemistry, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant porcine IL‑1 beta /IL‑1F2
Ala115-Pro267
Accession # P26889

Specificity

Detects porcine IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45%‑75% cross‑reactivity with recombinant human IL‑1 beta, recombinant mouse IL‑1 beta, recombinant rat IL‑1 beta, recombinant feline IL‑1 beta, recombinant canine IL‑1 beta, recombinant equine IL‑1 beta, recombinant guinea pig IL‑1 beta, recombinant rabbit IL‑1 beta, and recombinant rhesus monkey IL‑1 beta  is observed and less than 15% cross-reactivity with recombinant cotton rat IL-1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Porcine IL‑1 beta /IL‑1F2 Antibody

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutrali-zation by Porcine IL‑1 beta /IL‑1F2 Antibody.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutrali-zation by Porcine IL‑1 beta /IL‑1F2 Antibody.

Recombinant Porcine IL-1 beta /IL-1F2 (Catalog # 681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND50 is typically 4-20 µg/mL.
Detection of Porcine IL-1 beta /IL-1F2 by Western Blot

Detection of Porcine IL-1 beta /IL-1F2 by Western Blot

Silencing of TREM2 increases the production of inflammatory cytokines and type I interferons.(A) PAMs were transfected with siNC or siTREM2-1 for 24 h, gene expression of IL-1 beta, IL-6, IL-8, TNF-alpha, IFN-alpha, and IFN-beta are shown using qRT-PCR. (B) Protein expression of IL-1 beta, IL-10, IL-4, and TNF-alpha are shown, as detected by western blot analysis in the TREM2 knockdown assay. GAPDH is shown as an internal control. (C) PAMs were transfected with siNC or siTREM2-1 for 24 h and infected with PPRSV (MOI = 1). Cells were collected at the indicated periods. Proinflammatory cytokine (IL-1 beta, IL-6, and IL-8) and type I interferons (IFN-alpha and IFN-beta ) mRNA expression is shown, as detected by qRT-PCR. (D) PAMs were treated as described in A and infected with PRRSV at different MOIs (0.5 or 1), transcription of IL-1 beta, TNF-alpha, and IFN-beta is shown, as measured by qRT-PCR. Data are representative of the results of three independent experiments (mean ± SE). Significant differences compared to the control group are denoted by * (P <.05), ** (P <.01) and *** (P <.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32401783), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Porcine IL‑1 beta /IL‑1F2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI)

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 4-20 µg/mL in the presence of 20 ng/mL Recombinant Porcine IL‑1 beta /IL‑1F2.

Reviewed Applications

Read 1 review rated 5 using AF681 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta.

References

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Huether, M. et al. (1993) Gene 129:285.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

P26889

Additional IL-1 beta/IL-1F2 Products

Product Documents for Porcine IL‑1 beta /IL‑1F2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Porcine IL‑1 beta /IL‑1F2 Antibody

For research use only

Citations for Porcine IL‑1 beta /IL‑1F2 Antibody

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  • Porcine IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Porcine
    Verified Customer | Posted 11/07/2017
    We used MAB6911 as the capture antibody for Porcine IL-1b in a sandwich ELISA. Biotinylated AF681 was used as the detection antibody. The antigen standard was 681-PI. We were able to quantify serum and plasma samples.
    Porcine IL‑1 beta /IL‑1F2 Antibody AF681

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