Porcine IL-1 beta /IL-1F2 Antibody

Catalog # Availability Size / Price Qty
AF681
AF681-SP
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutrali-zation by Porcine IL‑1 beta /IL‑1F2 Antibody.
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Citations (4)
FAQs
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Reviews (1)

Porcine IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45%‑75% cross‑reactivity with recombinant human IL‑1 beta, recombinant mouse IL‑1 beta, recombinant rat IL‑1 beta, recombinant feline IL‑1 beta, recombinant canine IL‑1 beta, recombinant equine IL‑1 beta, recombinant guinea pig IL‑1 beta, recombinant rabbit IL‑1 beta, and recombinant rhesus monkey IL‑1 beta  is observed and less than 15% cross-reactivity with recombinant cotton rat IL-1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant porcine IL‑1 beta /IL‑1F2
Ala115-Pro267
Accession # P26889
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI)
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 4-20 µg/mL in the presence of 20 ng/mL Recombinant Porcine IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutrali-zation by Porcine IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutrali-zation by Porcine IL‑1 beta /IL‑1F2 Antibody. Recombinant Porcine IL-1 beta /IL-1F2 (Catalog # 681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND50 is typically 4-20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta.

References
  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Huether, M. et al. (1993) Gene 129:285.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

Citations for Porcine IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. TREM2 suppresses the proinflammatory response to facilitate PRRSV infection via PI3K/NF-kappaB signaling
    Authors: Z Zhu, X Zhang, W Dong, X Wang, S He, H Zhang, X Wang, R Wei, Y Chen, X Liu, C Guo
    PLoS Pathog., 2020;16(5):e1008543.
    Species: Porcine
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation
  2. NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination
    Authors: HS Park, G Liu, SN Thulasi Ra, SL Landreth, Q Liu, Y Zhou
    J. Virol., 2018;0(0):.
    Species: Porcine
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
    Authors: Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH
    Eur. Heart J., 2008;29(12):1578-90.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Induction of interleukin-1 in articular cartilage by explantation and cutting.
    Authors: Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J
    Arthritis Rheum., 2004;50(8):2539-46.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Neutralization

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Porcine IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/07/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Porcine

We used MAB6911 as the capture antibody for Porcine IL-1b in a sandwich ELISA. Biotinylated AF681 was used as the detection antibody. The antigen standard was 681-PI. We were able to quantify serum and plasma samples.