Porcine IL-6 Quantikine ELISA Kit

  (36 citations)     
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  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    4.3 pg/mL
  • Assay Range
    18.8 - 1,200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant porcine IL-6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Porcine IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IL-6 and antibodies raised against the recombinant factor. The immunoassay has been shown to accurately quantitate recombinant porcine IL-6. Results obtained using natural porcine IL-6 show dose response curves that are parallel to the standard curves obtained using recombinant Quantikine Porcine IL-6 kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-6.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 54.5 123 674 52.9 127 665
Standard Deviation 2.8 5.4 17.6 3.9 7.5 30.1
CV% 5.1 4.4 2.6 7.4 5.9 4.5

Recovery

The recovery of porcine IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 102 95-109
EDTA Plasma (n=4) 97 89-104
Heparin Plasma (n=4) 97 85-103
Serum (n=4) 96 87-110
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of porcine IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
 IL-6 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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Species
Sample Type
  1. PKD regulates actin polymerization, neutrophil deformability, and transendothelial migration in response to fMLP and trauma
    Authors: C Wille, T Eiseler, ST Langenberg, J Richter, K Mizuno, P Radermache, U Knippschil, M Huber-Lang, T Seufferlei, S Paschke
    J. Leukoc. Biol., 2018;0(0):.
    Species: Porcine
    Sample Type: Plasma
  2. Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome
    Authors: M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen
    BMC Vet. Res., 2018;14(1):83.
    Species: Porcine
    Sample Type: Serum
  3. An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome
    Authors: Angela J Westover
    J Diabetes Res, 2016;2016(0):3486727.
    Species: Porcine
    Sample Type: Plasma
  4. Characterization of blunt chest trauma in a long-term porcine model of severe multiple trauma
    Sci Rep, 2016;6(0):39659.
    Species: Porcine
    Sample Type: Serum
  5. Low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs.
    Authors: Ziebart A, Hartmann E, Thomas R, Liu T, Duenges B, Schad A, Bodenstein M, Thal S, David M
    Respir Res, 2014;15(0):101.
    Species: Porcine
    Sample Type: Plasma
  6. Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs.
    Authors: Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A
    Crit. Care Med., 2012;40(1):162-8.
    Species: Porcine
    Sample Type: Serum
  7. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
    Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
    Crit. Care Med., 2010;38(6):1467-74.
    Species: Porcine
    Sample Type: Plasma
  8. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Authors: Jeong HJ, Song YJ, Lee SW
    Clin. Vaccine Immunol., 2010;17(4):503-12.
    Species: Porcine
    Sample Type: Cell Culture Supernates
  9. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
    Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
    FASEB J., 2010;24(3):712-22.
    Species: Porcine
    Sample Type: Plasma
  10. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
    Authors: McILwain RB, Timpa JG, Kurundkar AR
    Lab. Invest., 2010;90(1):128-39.
    Species: Porcine
    Sample Type: Plasma
  11. Mutations in classical swine fever virus NS4B affect virulence in swine.
    Authors: Fernandez-Sainz I, Gladue DP, Holinka LG
    J. Virol., 2010;84(3):1536-49.
    Species: Porcine
    Sample Type: Cell Culture Supernates
  12. Effect of linezolid compared with glycopeptides in methicillin-resistant Staphylococcus aureus severe pneumonia in piglets.
    Authors: Luna CM, Bruno DA, Garcia-Morato J, Mann KC, Risso Patron J, Sagardia J, Absi R, Garcia Bottino M, Marchetti D, Famiglietti A, Baleztena M, Biancolini C
    Chest, 2009;135(6):1564-71.
    Species: Porcine
    Sample Type: Serum
  13. Bbeta15-42 (FX06) reduces pulmonary, myocardial, liver, and small intestine damage in a pig model of hemorrhagic shock and reperfusion.
    Authors: Roesner JP, Petzelbauer P, Koch A, Tran N, Iber T, Vagts DA, Scheeren TW, Vollmar B, Noldge-Schomburg GE, Zacharowski K
    Crit. Care Med., 2009;37(2):598-605.
    Species: Porcine
    Sample Type: Plasma
  14. Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
    Authors: Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE
    Infect. Immun., 2009;77(2):725-32.
    Species: Porcine
    Sample Type: Whole Blood
  15. Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
    Authors: Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG
    J. Interferon Cytokine Res., 2009;0(0):.
    Species: Porcine
    Sample Type: Plasma
  16. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
    Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
    J. Immunol., 2008;180(9):6346-53.
    Species: Porcine
    Sample Type: Cell Culture Supernates
  17. Effects of glucocorticoids in ventilated piglets with severe pneumonia.
    Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
    Eur. Respir. J., 2008;32(4):1037-46.
    Species: Porcine
    Sample Type: Serum
  18. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
    Authors: Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M
    Acta Vet. Scand., 2008;50(0):32.
    Species: Porcine
    Sample Type: Serum
  19. The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury.
    Authors: Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K
    Crit. Care Med., 2007;35(7):1730-5.
    Species: Porcine
    Sample Type: Plasma
  20. Sex difference in link between interleukin-6 and stress.
    Authors: Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH
    Endocrinology, 2007;148(8):3758-64.
    Species: Porcine
    Sample Type: Plasma
  21. Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation.
    Authors: Luna CM, Baquero S, Gando S, Patron JR, Morato JG, Sibila O, Absi R, Famiglietti A, Vay CA, Von Stecher F, Agusti C, Torres A
    Chest, 2007;132(2):523-31.
    Species: Porcine
    Sample Type: Serum
  22. Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
    Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
    Eur. Respir. J., 2007;30(6):1167-72.
    Species: Porcine
    Sample Type: Serum
  23. A novel antidote-controlled anticoagulant reduces thrombin generation and inflammation and improves cardiac function in cardiopulmonary bypass surgery.
    Authors: Nimjee SM, Keys JR, Pitoc GA, Quick G, Rusconi CP, Sullenger BA
    Mol. Ther., 2006;14(3):408-15.
    Species: Porcine
    Sample Type: Plasma
  24. Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages.
    Authors: Moller S, Lauridsen C
    Cytokine, 2006;35(1):6-12.
    Species: Porcine
    Sample Type: Cell Culture Supernates
  25. Effects of therapeutic bronchoalveolar lavage and partial liquid ventilation on meconium-aspirated newborn piglets.
    Authors: Jeng MJ, Soong WJ, Lee YS, Chang HL, Shen CM, Wang CH, Yang SS, Hwang B
    Crit. Care Med., 2006;34(4):1099-105.
    Species: Porcine
    Sample Type: Serum
  26. Complete overlap of interleukin-31 receptor A and oncostatin M receptor beta in the adult dorsal root ganglia with distinct developmental expression patterns.
    Authors: Bando T, Morikawa Y, Komori T, Senba E
    Neuroscience, 2006;142(4):1263-71.
    Species: Porcine
    Sample Type: Plasma
  27. Effects of heparin-binding protein (CAP37/azurocidin) in a porcine model of Actinobacillus pleuropneumoniae-induced pneumonia.
    Authors: Lauritzen B, Lykkesfeldt J, Djurup R, Flodgaard H, Svendsen O
    Pharmacol. Res., 2005;51(6):509-14.
    Species: Porcine
    Sample Type: Plasma
  28. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide.
    Authors: Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X
    J. Nutr., 2005;135(2):239-44.
    Species: Porcine
    Sample Type: Serum
  29. Protection against acute porcine lung ischemia/reperfusion injury by systemic preconditioning via hind limb ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Albrecht S, Wagner F, Knaut M, Matschke K
    Transpl. Int., 2005;18(2):198-205.
    Species: Porcine
    Sample Type: Plasma
  30. Adiponectin differentially regulates cytokines in porcine macrophages.
    Authors: Wulster-Radcliffe MC, Ajuwon KM, Wang J, Christian JA, Spurlock ME
    Biochem. Biophys. Res. Commun., 2004;316(3):924-9.
    Species: Porcine
    Sample Type: Cell Culture Supernates
  31. Attenuation of reperfusion-induced systemic inflammation by preconditioning with nitric oxide in an in situ porcine model of normothermic lung ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Wagner FM, Gulielmos V, Matschke K, Knaut M
    Chest, 2004;125(6):2253-9.
    Species: Porcine
    Sample Type: Plasma
  32. Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells.
    Authors: Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP
    J. Gen. Virol., 2004;85(0):1029-37.
    Species: Porcine
    Sample Type: Cell Culture Supernates
  33. Standardized lung recruitment during high frequency and conventional ventilation: similar pathophysiologic and inflammatory responses in an animal model of respiratory distress syndrome.
    Authors: Krishnan RK, Meyers PA, Worwa C, Goertz R, Schauer G, Mammel MC
    Intensive Care Med, 2004;30(6):1195-203.
    Species: Porcine
    Sample Type: Serum
  34. Decreased protein accretion in pigs with viral and bacterial pneumonia is associated with increased myostatin expression in muscle.
    Authors: Escobar J, Van Alstine WG, Baker DH
    J. Nutr., 2004;134(11):3047-53.
    Species: Porcine
    Sample Type: Serum
  35. Chronic porcine two-hit model with hemorrhagic shock and Pseudomonas aeruginosa sepsis.
    Authors: Eissner B, Matz K, Smorodchenko A, Roschmann A, v Specht BU
    Eur Surg Res, 2002;34(1):61-7.
    Species: Porcine
    Sample Type: Plasma
  36. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
    Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
    Mol. Ther., 212;20(6):1204-11.
    Species: Porcine
    Sample Type: Plasma
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