Rat CD19 Antibody Summary
Accession # NP_001013255
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat CD19 by Western Blot. Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CD19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD19 at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD19 (also surface antigen B4 and Leu12) is a 95-110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19-547). Based on mouse, it contains a 269 aa extracellular region (aa 19-287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20-113 and 171-271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19-287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.
Citation for Rat CD19 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Telophase correction refines division orientation in stratified epithelia
Authors: KJ Lough, KM Byrd, CP Descovich, DC Spitzer, AJ Bergman, GM Beaudoin, LF Reichardt, SE Williams
Sample Types: Whole Tissue
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