IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals.
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
5 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum)
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Product Summary for Rat IL-4 Quantikine ELISA Kit
The Quantikine Rat IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-4 accurately. Results obtained using natural rat IL-4 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-4.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Rat
Specificity
Natural and recombinant rat IL-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 48.2 | 111 | 721 | 47.7 | 112 | 742 |
| Standard Deviation | 2.5 | 4.9 | 23.6 | 3.9 | 6.1 | 31.8 |
| CV% | 5.2 | 4.4 | 3.3 | 8.2 | 5.4 | 4.3 |
Recovery for Rat IL-4 Quantikine ELISA Kit
The recovery of rat IL-4 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 110 | 104-117 |
| Serum (n=7) | 94 | 88-101 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IL-4 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Rat IL-4 Quantikine ELISA Kit
Rat IL-4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-4
Long Name
Interleukin 4
Alternate Names
BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12
Entrez Gene IDs
Gene Symbol
IL4
Additional IL-4 Products
Product Documents for Rat IL-4 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat IL-4 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Rat IL-4 Quantikine ELISA Kit
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Customer Reviews for Rat IL-4 Quantikine ELISA Kit (3)
5 out of 5
3 Customer Ratings
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Sample Tested: SerumVerified Customer | Posted 12/11/2021
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Sample Tested: simulated body fluidVerified Customer | Posted 12/09/2020
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Sample Tested: simulated body fluidVerified Customer | Posted 03/13/2020We tested IL-4 levels in a release assay performed in simulated body fluid. The standard curve was excellent and the CV values were all very low. We have used kits from other companies and found RnD systems kits to be superior to all other companies. The kits from RnD systems result in much lower background levels and much lower CV levels, which really helps with consistency and repeatability. The image shows the standard curve and the green outlines the low CV values.
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Protocols
View specific protocols for Rat IL-4 Quantikine ELISA Kit (R4000):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-4 Signaling Pathways
IL-4 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
