Rat IL-6 Quantikine ELISA Kit

(44 citations)   
  • Assay Length
    4.5 hours
  • Specificity
    Natural and recombinant rat IL-6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-6 in cell culture supernates, rat serum, and plasma. It contains E. coli-expressed recombinant rat IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat IL-6 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-6.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation10.622.446.213.421.769.2


The recovery of rat IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 103 85-118
EDTA Plasma (n=5) 96 81-111
Serum (n=5) 97 91-105
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
Rat IL-6 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
    • Long Name
      Interleukin 6
    • Entrez Gene IDs
      3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit);
    • Alternate Names
      B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta 2; IFN-beta-2; IL6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A;
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 44
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    Sample Type
    1. Effect of Silodosin, an Alpha1A-Adrenoceptor Antagonist, on Ventral Prostatic Hyperplasia in the Spontaneously Hypertensive Rat.
      Authors: Shimizu S, Shimizu T, Tsounapi P, Higashi Y, Martin D, Nakamura K, Honda M, Inoue K, Saito M
      PLoS ONE, 2015;10(8):e0133798.
    2. IL-17A is implicated in lipopolysaccharide-induced neuroinflammation and cognitive impairment in aged rats via microglial activation.
      Authors: Sun J, Zhang S, Zhang X, Zhang X, Dong H, Qian Y
      J Neuroinflammation, 2015;12(0):165.
    3. Melatonin attenuates the TLR4-mediated inflammatory response through MyD88- and TRIF-dependent signaling pathways in an in vivo model of ovarian cancer.
      Authors: Chuffa L, Fioruci-Fontanelli B, Mendes L, Ferreira Seiva F, Martinez M, Favaro W, Domeniconi R, Pinheiro P, Delazari Dos Santos L, Martinez F
      BMC Cancer, 2015;15(0):34.
      Species: Rat
      Sample Type: Tissue Supernate
    4. Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy.
      Authors: Ahmed M, Kumar G, Navarro G, Wang Y, Gourevitch S, Moussa M, Rozenblum N, Levchenko T, Galun E, Torchilin V, Goldberg S
      PLoS ONE, 2015;10(7):e0128910.
      Species: Rat
      Sample Type: Serum
    5. Iron-hepcidin dysmetabolism, anemia and renal hypoxia, inflammation and fibrosis in the remnant kidney rat model.
      Authors: Garrido P, Ribeiro S, Fernandes J, Vala H, Bronze-da-Rocha E, Rocha-Pereira P, Belo L, Costa E, Santos-Silva A, Reis F
      PLoS ONE, 2015;10(4):e0124048.
      Species: Rat
      Sample Type: Serum
    6. Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat.
      Authors: Wang Z, Huang W, Zuo Z
      J Neuroinflammation, 2014;11(0):56.
      Species: Rat
      Sample Type: Whole Tissue
    7. Microglia trigger astrocyte-mediated neuroprotection via purinergic gliotransmission.
      Authors: Shinozaki Y, Nomura M, Iwatsuki K, Moriyama Y, Gachet C, Koizumi S
      Sci Rep, 2014;4(0):4329.
      Species: Rat
      Sample Type: Cell Culture Supernates
    8. Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop.
      Authors: Bustamante M, Fernandez-Verdejo R, Jaimovich E, Buvinic S
      Am J Physiol Endocrinol Metab, 2014;306(8):E869-82.
      Species: Rat
      Sample Type: Cell Culture Supernates
    9. Mechanical ventilation enhances HMGB1 expression in an LPS-induced lung injury model.
      Authors: Ding N, Wang F, Xiao H, Xu L, She S
      PLoS ONE, 2013;8(9):e74633.
      Species: Rat
      Sample Type: BALF
    10. A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
      Cytokine, 2013;61(2):614-21.
      Species: Rat
      Sample Type: BALF
    11. Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture.
      Authors: Francque S, Laleman W, Verbeke L, Van Steenkiste C, Casteleyn C, Kwanten W, Van Dyck C
      Lab Invest, 2012;92(10):1428-39.
      Species: Rat
      Sample Type: Serum
    12. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
      J Neuroinflammation, 2012;9(0):181.
      Species: Rat
      Sample Type: Tissue Homogenates
    13. Vipera aspis venom reduces lethality and down-regulates tumor necrosis factor-alpha in a rat model of LPS-induced sepsis.
      Authors: Frolkis I, Klein Y, Locker C, Adi N, Dahan E, Uretzsky G, Shapira I, Sorkine P
      Cytokine, 2010;49(3):319-24.
      Species: Rat
      Sample Type: Serum
    14. Mechanism of hepatoprotection in proestrus female rats following trauma-hemorrhage: heme oxygenase-1-derived normalization of hepatic inflammatory responses.
      Authors: Yang S, Hu S, Chen J, Choudhry MA, Rue LW, Bland KI, Chaudry IH
      J. Leukoc. Biol., 2009;85(6):1015-26.
      Species: Rat
      Sample Type: Serum
    15. Hypercapnic acidosis attenuates shock and lung injury in early and prolonged systemic sepsis.
      Authors: Costello J, Higgins B, Contreras M, Chonghaile MN, Hassett P, O'Toole D, Laffey JG
      Crit. Care Med., 2009;37(8):2412-20.
      Species: Rat
      Sample Type: BALF
    16. In vivo Angiotensin II AT1 receptor blockade selectively inhibits LPS-induced innate immune response and ACTH release in rat pituitary gland.
      Authors: Sanchez-Lemus E, Benicky J, Pavel J, Saavedra JM
      Brain Behav. Immun., 2009;23(7):945-57.
      Species: Rat
      Sample Type: Plasma
    17. Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha.
      Authors: Xu J, Kim HT, Ma Y, Zhao L, Zhai L, Kokorina N, Wang P, Messina JL
      Endocrinology, 2008;149(5):2369-82.
      Species: Rat
      Sample Type: Serum
    18. JAK/STAT3 pathway is activated in spinal cord microglia after peripheral nerve injury and contributes to neuropathic pain development in rat.
      Authors: Dominguez E, Rivat C, Pommier B, Mauborgne A, Pohl M
      J. Neurochem., 2008;107(1):50-60.
      Species: Rat
      Sample Type: Tissue Homogenates
    19. Antithrombin inhibits bronchoalveolar activation of coagulation and limits lung injury during Streptococcus pneumoniae pneumonia in rats.
      Authors: Choi G, Hofstra JJ, Roelofs JJ, Rijneveld AW, Bresser P, van der Zee JS, Florquin S, van der Poll T, Levi M, Schultz MJ
      Crit. Care Med., 2008;36(1):204-10.
      Species: Rat
      Sample Type: BALF
    20. Regulation of liver cytochrome P450 by activation of brain dopaminergic system: physiological and pharmacological implications.
      Authors: Wojcikowski J, Golembiowska K, Daniel WA
      Biochem. Pharmacol., 2008;76(2):258-67.
      Species: Rat
      Sample Type: Plasma
    21. Induction of type 2 iodothyronine deiodinase in the mediobasal hypothalamus by bacterial lipopolysaccharide: role of corticosterone.
      Authors: Sanchez E, Singru PS, Fekete C, Lechan RM
      Endocrinology, 2008;149(5):2484-93.
      Species: Rat
      Sample Type: Serum
    22. Role of central beta-adrenergic receptors in regulating proinflammatory cytokine responses to a peripheral bacterial challenge.
      Authors: Johnson JD, Cortez V, Kennedy SL, Foley TE, Hanson H, Fleshner M
      Brain Behav. Immun., 2008;22(7):1078-86.
      Species: Rat
      Sample Type: Plasma
    23. Cell-based angiopoietin-1 gene therapy for acute lung injury.
      Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
      Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
      Species: Rat
      Sample Type: BALF
    24. Anti-rat soluble IL-6 receptor antibody down-regulates cardiac IL-6 and improves cardiac function following trauma-hemorrhage.
      Authors: Yang S, Hu S, Choudhry MA, Rue LW, Bland KI, Chaudry IH
      J. Mol. Cell. Cardiol., 2007;42(3):620-30.
      Species: Rat
      Sample Type: Plasma
    25. Natural anticoagulants limit lipopolysaccharide-induced pulmonary coagulation but not inflammation.
      Authors: Choi G, Vlaar AP, Schouten M, Van't Veer C, van der Poll T, Levi M, Schultz MJ
      Eur. Respir. J., 2007;30(3):423-8.
      Species: Rat
      Sample Type: Tissue Homogenates
    26. Changes of gene expression of iron regulatory proteins during turpentine oil-induced acute-phase response in the rat.
      Authors: Sheikh N, Dudas J, Ramadori G
      Lab. Invest., 2007;87(7):713-25.
      Species: Rat
      Sample Type: Serum
    27. Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation.
      Authors: Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(5):L1183-93.
      Species: Rat
      Sample Type: BALF
    28. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway.
      Authors: Yu HP, Hsieh YC, Suzuki T, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH
      J. Leukoc. Biol., 2007;82(3):774-80.
      Species: Rat
      Sample Type: Tissue Homogenates
    29. ACE mediates ventilator-induced lung injury in rats via angiotensin II but not bradykinin.
      Authors: Wosten-van Asperen RM, Lutter R, Haitsma JJ, Merkus MP, van Woensel JB, van der Loos CM, Florquin S, Lachmann B, Bos AP
      Eur. Respir. J., 2007;31(2):363-71.
      Species: Rat
      Sample Type: BALF
    30. Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure.
      Authors: Holly MK, Dear JW, Hu X, Schechter AN, Gladwin MT, Hewitt SM, Yuen PS, Star RA
      Kidney Int., 2006;70(3):496-506.
      Species: Rat
      Sample Type: Serum
    31. Glucosamine administration during resuscitation improves organ function after trauma hemorrhage.
      Authors: Yang S, Zou LY, Bounelis P, Chaudry I, Chatham JC, Marchase RB
      Shock, 2006;25(6):600-7.
      Species: Rat
      Sample Type: Plasma
    32. Suppression of ocular inflammation in endotoxin-induced uveitis by inhibiting nonproteolytic activation of prorenin.
      Authors: Satofuka S, Ichihara A, Nagai N, Yamashiro K, Koto T, Shinoda H, Noda K, Ozawa Y, Inoue M, Tsubota K, Suzuki F, Oike Y, Ishida S
      Invest. Ophthalmol. Vis. Sci., 2006;47(6):2686-92.
      Species: Rat
      Sample Type: Tissue Homogenates
    33. Effects of gabexate mesilate on serum inflammatory cytokines in rats with acute necrotizing pancreatitis.
      Authors: Chen CC, Wang SS, Tsay SH, Lee FY, Lu RH, Chang FY, Lee SD
      Cytokine, 2006;33(2):95-9.
      Species: Rat
      Sample Type: Serum
    34. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
      Authors: Barkho BZ, Song H, Aimone JB
      Stem Cells Dev., 2006;15(3):407-21.
      Species: Rat
      Sample Type: Cell Culture Supernates
    35. The cytokine interleukin-6 is sufficient but not necessary to mimic the peripheral conditioning lesion effect on axonal growth.
      Authors: Cao Z, Gao Y, Bryson JB, Hou J, Chaudhry N, Siddiq M, Martinez J, Spencer T, Carmel J, Hart RB, Filbin MT
      J. Neurosci., 2006;26(20):5565-73.
      Species: Rat
      Sample Type: Cell Culture Supernates
    36. Developmental changes induced by graded prenatal systemic hypoxic-ischemic insults in rats.
      Authors: Robinson S, Petelenz K, Li Q, Cohen ML, Dechant A, Tabrizi N, Bucek M, Lust D, Miller RH
      Neurobiol. Dis., 2005;18(3):568-81.
      Species: Rat
      Sample Type: Amniotic Fluid
    37. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
      Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
      Crit. Care Med., 2005;33(10):2317-22.
      Species: Rat
      Sample Type: BALF
    38. A method for measuring multiple cytokines from small samples.
      Authors: O&amp;amp;apos;Connor KA
      Brain Behav. Immun., 2004;18(3):274-80.
      Species: Rat
      Sample Type: CSF
    39. Anti-inflammatory effects of alpha-melanocyte-stimulating hormone against rat endotoxin-induced uveitis and the time course of inflammatory agents in aqueous humor.
      Authors: Nishida T, Miyata S, Itoh Y, Mizuki N, Ohgami K, Shiratori K, Ilieva IB, Ohno S, Taylor AW
      Int. Immunopharmacol., 2004;4(8):1059-66.
      Species: Rat
      Sample Type: Aqueous humor
    40. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
      Authors: Schubert T, Horch RE
      Ann. Surg., 2004;240(2):340-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    41. HIV-1 gp120 stimulates proinflammatory cytokine-mediated pain facilitation via activation of nitric oxide synthase-I (nNOS).
      Authors: Holguin A, O&amp;amp;apos;Connor KA, Biedenkapp J, Campisi J, Wieseler-Frank J, Milligan ED, Hansen MK, Spataro L, Maksimova E, Bravmann C, Martin D, Fleshner M, Maier SF, Watkins LR
      Pain, 2004;110(3):517-30.
      Species: Rat
      Sample Type: CSF
    42. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
      Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
      Endocrinology, 2004;145(9):4084-93.
      Species: Rat
      Sample Type: Tissue Homogenates
    43. Differential expression of cytokine genes and inducible nitric oxide synthase induced by opacity phenotype variants of Streptococcus pneumoniae during acute otitis media in the rat.
      Authors: Long JP, Tong HH, Shannon PA, DeMaria TF
      Infect. Immun., 2003;71(10):5531-40.
      Species: Rat
      Sample Type: middle ear effusion
    44. Iron intake increases infarct volume after permanent middle cerebral artery occlusion in rats.
      Authors: Castellanos M, Puig N, Carbonell T, Castillo J, Martinez J, Rama R, Davalos A
      Brain Res., 2002;952(1):1-6.
      Species: Rat
      Sample Type: Serum
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