Rat IL-6 Quantikine ELISA Kit

(54 citations)   
  • Assay Length
    4.5 hours
  • Specificity
    Natural and recombinant rat IL-6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-6 in cell culture supernates, rat serum, and plasma. It contains E. coli-expressed recombinant rat IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat IL-6 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-6.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020222728
Mean1202561028134268987
Standard Deviation10.622.446.213.421.769.2
CV%8.88.84.5108.17

Recovery

The recovery of rat IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 103 85-118
EDTA Plasma (n=5) 96 81-111
Serum (n=5) 97 91-105
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
Rat IL-6 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
    • Long Name
      Interleukin 6
    • Entrez Gene IDs
      3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit);
    • Alternate Names
      B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    54 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Maternal-placental-fetal biodistribution of multimodal polymeric nanoparticles in a pregnant rat model in mid and late gestation
      Authors: D Ho, JW Leong, RC Crew, M Norret, MJ House, PJ Mark, BJ Waddell, KS Iyer, JA Keelan
      Sci Rep, 2017;7(1):2866.
      Species: Rat
      Sample Type: Plasma
    2. Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
      Authors: MY Ahn, BJ Kim, HJ Kim, JS Hwang, YS Jung, KK Park
      Biomater Res, 2017;21(0):5.
      Species: Rat
      Sample Type: Serum
    3. The Effects of Remifentanil on Expression of High Mobility Group Box 1 in Septic Rats
      Authors: KH Seo, JW Choi, HS Jung, H Yoo, JD Joo
      J. Korean Med. Sci, 2017;32(3):542-551.
      Species: Rat
      Sample Type: Serum
    4. Short-term treatment with taurolidine is associated with liver injury
      Authors: R Fahrner, A Möller, AT Press, A Kortgen, M Kiehntopf, F Rauchfuss, U Settmacher, AS Mosig
      BMC Pharmacol Toxicol, 2017;18(1):61.
      Species: Rat
      Sample Type: Serum
    5. Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
      Authors: K Yan, Y Shen
      Mol Med Rep, 2017;0(0):.
      Species: Rat
      Sample Type: Serum
    6. Melatonin attenuates the TLR4-mediated inflammatory response through MyD88- and TRIF-dependent signaling pathways in an in vivo model of ovarian cancer.
      Authors: Chuffa L, Fioruci-Fontanelli B, Mendes L, Ferreira Seiva F, Martinez M, Favaro W, Domeniconi R, Pinheiro P, Delazari Dos Santos L, Martinez F
      BMC Cancer, 2015;15(0):34.
      Species: Rat
      Sample Type: Tissue Culture Supernates
    7. IL-17A is implicated in lipopolysaccharide-induced neuroinflammation and cognitive impairment in aged rats via microglial activation.
      Authors: Sun J, Zhang S, Zhang X, Zhang X, Dong H, Qian Y
      J Neuroinflammation, 2015;12(0):165.
    8. Effect of Silodosin, an Alpha1A-Adrenoceptor Antagonist, on Ventral Prostatic Hyperplasia in the Spontaneously Hypertensive Rat.
      Authors: Shimizu S, Shimizu T, Tsounapi P, Higashi Y, Martin D, Nakamura K, Honda M, Inoue K, Saito M
      PLoS ONE, 2015;10(8):e0133798.
    9. Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy.
      Authors: Ahmed M, Kumar G, Navarro G, Wang Y, Gourevitch S, Moussa M, Rozenblum N, Levchenko T, Galun E, Torchilin V, Goldberg S
      PLoS ONE, 2015;10(7):e0128910.
      Species: Rat
      Sample Type: Serum
    10. Iron-hepcidin dysmetabolism, anemia and renal hypoxia, inflammation and fibrosis in the remnant kidney rat model.
      Authors: Garrido P, Ribeiro S, Fernandes J, Vala H, Bronze-da-Rocha E, Rocha-Pereira P, Belo L, Costa E, Santos-Silva A, Reis F
      PLoS ONE, 2015;10(4):e0124048.
      Species: Rat
      Sample Type: Serum
    11. The extent of irradiation-induced long-term visceral organ damage depends on cranial/brain exposure.
      Authors: Boittin F, Denis J, Mayol J, Martigne P, Raffin F, Coulon D, Grenier N, Drouet M, Herodin F
      PLoS ONE, 2015;10(4):e0122900.
      Species: Rat
      Sample Type: Plasma
    12. Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3.
      Authors: Wilson C, Mann J, Walsh M, Perrugoria M, Oakley F, Wright M, Brignole C, Di Paolo D, Perri P, Ponzoni M, Karin M, Mann D
      PLoS ONE, 2014;9(1):e83391.
      Species: Rat
      Sample Type: Cell Culture Supernates
    13. Catalpol ameliorates sodium taurocholate-induced acute pancreatitis in rats via inhibiting activation of nuclear factor kappa B.
      Authors: Xiao W, Yin G, Fan Y, Qiu L, Cang X, Yu G, Hu Y, Xing M, Wu D, Wang X, Hu G, Wan R
      Int J Mol Sci, 2014;15(7):11957-72.
      Species: Rat
      Sample Type: Serum
    14. Microglia trigger astrocyte-mediated neuroprotection via purinergic gliotransmission.
      Authors: Shinozaki Y, Nomura M, Iwatsuki K, Moriyama Y, Gachet C, Koizumi S
      Sci Rep, 2014;4(0):4329.
      Species: Rat
      Sample Type: Cell Culture Supernates
    15. Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat.
      Authors: Wang Z, Huang W, Zuo Z
      J Neuroinflammation, 2014;11(0):56.
      Species: Rat
      Sample Type: Whole Tissue
    16. Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop.
      Authors: Bustamante M, Fernandez-Verdejo R, Jaimovich E, Buvinic S
      Am J Physiol Endocrinol Metab, 2014;306(8):E869-82.
      Species: Rat
      Sample Type: Cell Culture Supernates
    17. Early cardiac changes in a rat model of prediabetes: brain natriuretic peptide overexpression seems to be the best marker.
      Authors: Nunes S, Soares E, Fernandes J, Viana S, Carvalho E, Pereira F, Reis F
      Cardiovasc Diabetol, 2013;12(0):44.
      Species: Rat
      Sample Type: Serum
    18. Targeting miR-21 inhibits in vitro and in vivo multiple myeloma cell growth.
      Authors: Leone E, Morelli E, Di Martino M, Amodio N, Foresta U, Gulla A, Rossi M, Neri A, Giordano A, Munshi N, Anderson K, Tagliaferri P, Tassone P
      Clin Cancer Res, 2013;19(8):2096-106.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
      Cytokine, 2013;61(2):614-21.
      Species: Rat
      Sample Type: BALF
    20. Mechanical ventilation enhances HMGB1 expression in an LPS-induced lung injury model.
      Authors: Ding N, Wang F, Xiao H, Xu L, She S
      PLoS ONE, 2013;8(9):e74633.
      Species: Rat
      Sample Type: BALF
    21. Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture.
      Authors: Francque S, Laleman W, Verbeke L, Van Steenkiste C, Casteleyn C, Kwanten W, Van Dyck C
      Lab Invest, 2012;92(10):1428-39.
      Species: Rat
      Sample Type: Serum
    22. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
      J Neuroinflammation, 2012;9(0):181.
      Species: Rat
      Sample Type: Tissue Homogenates
    23. Vipera aspis venom reduces lethality and down-regulates tumor necrosis factor-alpha in a rat model of LPS-induced sepsis.
      Authors: Frolkis I, Klein Y, Locker C, Adi N, Dahan E, Uretzsky G, Shapira I, Sorkine P
      Cytokine, 2010;49(3):319-24.
      Species: Rat
      Sample Type: Serum
    24. Mechanism of hepatoprotection in proestrus female rats following trauma-hemorrhage: heme oxygenase-1-derived normalization of hepatic inflammatory responses.
      Authors: Yang S, Hu S, Chen J, Choudhry MA, Rue LW, Bland KI, Chaudry IH
      J. Leukoc. Biol., 2009;85(6):1015-26.
      Species: Rat
      Sample Type: Serum
    25. In vivo Angiotensin II AT1 receptor blockade selectively inhibits LPS-induced innate immune response and ACTH release in rat pituitary gland.
      Authors: Sanchez-Lemus E, Benicky J, Pavel J, Saavedra JM
      Brain Behav. Immun., 2009;23(7):945-57.
      Species: Rat
      Sample Type: Plasma
    26. Hypercapnic acidosis attenuates shock and lung injury in early and prolonged systemic sepsis.
      Authors: Costello J, Higgins B, Contreras M, Chonghaile MN, Hassett P, O'Toole D, Laffey JG
      Crit. Care Med., 2009;37(8):2412-20.
      Species: Rat
      Sample Type: BALF
    27. Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha.
      Authors: Xu J, Kim HT, Ma Y, Zhao L, Zhai L, Kokorina N, Wang P, Messina JL
      Endocrinology, 2008;149(5):2369-82.
      Species: Rat
      Sample Type: Serum
    28. Regulation of liver cytochrome P450 by activation of brain dopaminergic system: physiological and pharmacological implications.
      Authors: Wojcikowski J, Golembiowska K, Daniel WA
      Biochem. Pharmacol., 2008;76(2):258-67.
      Species: Rat
      Sample Type: Plasma
    29. Induction of type 2 iodothyronine deiodinase in the mediobasal hypothalamus by bacterial lipopolysaccharide: role of corticosterone.
      Authors: Sanchez E, Singru PS, Fekete C, Lechan RM
      Endocrinology, 2008;149(5):2484-93.
      Species: Rat
      Sample Type: Serum
    30. Antithrombin inhibits bronchoalveolar activation of coagulation and limits lung injury during Streptococcus pneumoniae pneumonia in rats.
      Authors: Choi G, Hofstra JJ, Roelofs JJ, Rijneveld AW, Bresser P, van der Zee JS, Florquin S, van der Poll T, Levi M, Schultz MJ
      Crit. Care Med., 2008;36(1):204-10.
      Species: Rat
      Sample Type: BALF
    31. Role of central beta-adrenergic receptors in regulating proinflammatory cytokine responses to a peripheral bacterial challenge.
      Authors: Johnson JD, Cortez V, Kennedy SL, Foley TE, Hanson H, Fleshner M
      Brain Behav. Immun., 2008;22(7):1078-86.
      Species: Rat
      Sample Type: Plasma
    32. JAK/STAT3 pathway is activated in spinal cord microglia after peripheral nerve injury and contributes to neuropathic pain development in rat.
      Authors: Dominguez E, Rivat C, Pommier B, Mauborgne A, Pohl M
      J. Neurochem., 2008;107(1):50-60.
      Species: Rat
      Sample Type: Tissue Homogenates
    33. Natural anticoagulants limit lipopolysaccharide-induced pulmonary coagulation but not inflammation.
      Authors: Choi G, Vlaar AP, Schouten M, Van't Veer C, van der Poll T, Levi M, Schultz MJ
      Eur. Respir. J., 2007;30(3):423-8.
      Species: Rat
      Sample Type: Tissue Homogenates
    34. ACE mediates ventilator-induced lung injury in rats via angiotensin II but not bradykinin.
      Authors: Wosten-van Asperen RM, Lutter R, Haitsma JJ, Merkus MP, van Woensel JB, van der Loos CM, Florquin S, Lachmann B, Bos AP
      Eur. Respir. J., 2007;31(2):363-71.
      Species: Rat
      Sample Type: BALF
    35. Changes of gene expression of iron regulatory proteins during turpentine oil-induced acute-phase response in the rat.
      Authors: Sheikh N, Dudas J, Ramadori G
      Lab. Invest., 2007;87(7):713-25.
      Species: Rat
      Sample Type: Serum
    36. Cell-based angiopoietin-1 gene therapy for acute lung injury.
      Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
      Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
      Species: Rat
      Sample Type: BALF
    37. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway.
      Authors: Yu HP, Hsieh YC, Suzuki T, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH
      J. Leukoc. Biol., 2007;82(3):774-80.
      Species: Rat
      Sample Type: Tissue Homogenates
    38. Anti-rat soluble IL-6 receptor antibody down-regulates cardiac IL-6 and improves cardiac function following trauma-hemorrhage.
      Authors: Yang S, Hu S, Choudhry MA, Rue LW, Bland KI, Chaudry IH
      J. Mol. Cell. Cardiol., 2007;42(3):620-30.
      Species: Rat
      Sample Type: Plasma
    39. Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation.
      Authors: Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(5):L1183-93.
      Species: Rat
      Sample Type: BALF
    40. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
      Authors: Barkho BZ, Song H, Aimone JB
      Stem Cells Dev., 2006;15(3):407-21.
      Species: Rat
      Sample Type: Cell Culture Supernates
    41. Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure.
      Authors: Holly MK, Dear JW, Hu X, Schechter AN, Gladwin MT, Hewitt SM, Yuen PS, Star RA
      Kidney Int., 2006;70(3):496-506.
      Species: Rat
      Sample Type: Serum
    42. Suppression of ocular inflammation in endotoxin-induced uveitis by inhibiting nonproteolytic activation of prorenin.
      Authors: Satofuka S, Ichihara A, Nagai N, Yamashiro K, Koto T, Shinoda H, Noda K, Ozawa Y, Inoue M, Tsubota K, Suzuki F, Oike Y, Ishida S
      Invest. Ophthalmol. Vis. Sci., 2006;47(6):2686-92.
      Species: Rat
      Sample Type: Tissue Homogenates
    43. Effects of gabexate mesilate on serum inflammatory cytokines in rats with acute necrotizing pancreatitis.
      Authors: Chen CC, Wang SS, Tsay SH, Lee FY, Lu RH, Chang FY, Lee SD
      Cytokine, 2006;33(2):95-9.
      Species: Rat
      Sample Type: Serum
    44. Glucosamine administration during resuscitation improves organ function after trauma hemorrhage.
      Authors: Yang S, Zou LY, Bounelis P, Chaudry I, Chatham JC, Marchase RB
      Shock, 2006;25(6):600-7.
      Species: Rat
      Sample Type: Plasma
    45. The cytokine interleukin-6 is sufficient but not necessary to mimic the peripheral conditioning lesion effect on axonal growth.
      Authors: Cao Z, Gao Y, Bryson JB, Hou J, Chaudhry N, Siddiq M, Martinez J, Spencer T, Carmel J, Hart RB, Filbin MT
      J. Neurosci., 2006;26(20):5565-73.
      Species: Rat
      Sample Type: Cell Culture Supernates
    46. Developmental changes induced by graded prenatal systemic hypoxic-ischemic insults in rats.
      Authors: Robinson S, Petelenz K, Li Q, Cohen ML, Dechant A, Tabrizi N, Bucek M, Lust D, Miller RH
      Neurobiol. Dis., 2005;18(3):568-81.
      Species: Rat
      Sample Type: Amniotic Fluid
    47. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
      Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
      Crit. Care Med., 2005;33(10):2317-22.
      Species: Rat
      Sample Type: BALF
    48. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
      Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
      Endocrinology, 2004;145(9):4084-93.
      Species: Rat
      Sample Type: Tissue Homogenates
    49. HIV-1 gp120 stimulates proinflammatory cytokine-mediated pain facilitation via activation of nitric oxide synthase-I (nNOS).
      Authors: Holguin A, O&amp;amp;apos;Connor KA, Biedenkapp J, Campisi J, Wieseler-Frank J, Milligan ED, Hansen MK, Spataro L, Maksimova E, Bravmann C, Martin D, Fleshner M, Maier SF, Watkins LR
      Pain, 2004;110(3):517-30.
      Species: Rat
      Sample Type: CSF
    50. Anti-inflammatory effects of alpha-melanocyte-stimulating hormone against rat endotoxin-induced uveitis and the time course of inflammatory agents in aqueous humor.
      Authors: Nishida T, Miyata S, Itoh Y, Mizuki N, Ohgami K, Shiratori K, Ilieva IB, Ohno S, Taylor AW
      Int. Immunopharmacol., 2004;4(8):1059-66.
      Species: Rat
      Sample Type: Aqueous humor
    51. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
      Authors: Schubert T, Horch RE
      Ann. Surg., 2004;240(2):340-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    52. A method for measuring multiple cytokines from small samples.
      Authors: O&amp;amp;apos;Connor KA
      Brain Behav. Immun., 2004;18(3):274-80.
      Species: Rat
      Sample Type: CSF
    53. Differential expression of cytokine genes and inducible nitric oxide synthase induced by opacity phenotype variants of Streptococcus pneumoniae during acute otitis media in the rat.
      Authors: Long JP, Tong HH, Shannon PA, DeMaria TF
      Infect. Immun., 2003;71(10):5531-40.
      Species: Rat
      Sample Type: Tissue Secretion
    54. Iron intake increases infarct volume after permanent middle cerebral artery occlusion in rats.
      Authors: Castellanos M, Puig N, Carbonell T, Castillo J, Martinez J, Rama R, Davalos A
      Brain Res., 2002;952(1):1-6.
      Species: Rat
      Sample Type: Serum
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