Detects rat IL-7 R alpha /CD127 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IL-7 R alpha and less than 10% cross-reactivity with recombinant human IL-7 R alpha is observed.
Polyclonal Sheep IgG
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127 Glu21-Asp239 Accession # NP_001099888
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Rat IL‑7 R alpha /CD127 by Western Blot.
Western blot shows lysates of rat spleen tissue. PVDF Membrane was probed with 1 µg/mL of Rat IL‑7 R alpha /CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL‑7 R alpha /CD127 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with Allophycocyanin-conjugated Mouse Anti-Rat CD4 Antibody and either (A) Normal Sheep IgG Control (Catalog # 5-001-A) or (B) Sheep Anti-Rat IL‑7 R alpha /CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-7 R alpha/CD127
IL-7 R alpha (IL-7 receptor alpha; also CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 aa type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129‑222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21‑239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.
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