Rat IL-7R alpha/CD127 Antibody

Name: Rat IL-7R alpha/CD127 Antibody
Brand: R&D Systems
SKU: AF5607
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # AF5607

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all IL-7R alpha/CD127 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127
Glu21-Asp239
Accession # NP_001099888

Specificity

Detects rat IL-7 R alpha /CD127 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IL-7 R alpha and less than 10% cross-reactivity with recombinant human IL-7 R alpha is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Rat IL-7R alpha/CD127 Antibody

Detection of Rat IL‑7 R alpha /CD127 by Western Blot.

Western blot shows lysates of rat spleen tissue. PVDF Membrane was probed with 1 µg/mL of Rat IL-7 Ra/CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-7 Ra/CD127 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Allophycocyanin-conjugated Mouse Anti-Rat CD4 Antibody and either (A) Normal Sheep IgG Control (Catalog # 5-001-A) or (B) Sheep Anti-Rat IL-7 Ra/CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Applications for Rat IL-7R alpha/CD127 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Rat splenocytes

Western Blot

1 µg/mL
Sample: Rat spleen tissue

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-7R alpha/CD127

IL-7 R alpha (IL-7 receptor alpha; also CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 aa type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129‑222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21‑239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.