Nestin in Rat Cortical Stem Cells.
Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Rat Nestin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
Lendahl, U. et al. (1990) Cell 60:585.
Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
Tohyama, T. et al. (1992) Lab. Invest. 66:303.
Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
Frederiksen, K. et al. (1988) Neuron 1:439.
Cattaneo, E. et al. (1990) Nature 347:762.
Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
Rietze, R.L. et al. (2001) Nature 412:736.
Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
Zulewski, H. et al. (2001) Diabetes 50:521.
Lumelsky, N. et al. (2001) Science 292:1389.
Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
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