Recombinant Antibodies

Bio-Techne’s recombinant monoclonal antibodies display excellent sensitivity and consistency to reduce variability in your experiments.

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Biology introduces more than enough variability for researchers to worry about without adding inconsistent reagents to the equation. Start minimizing one major source of experimental variability in your research by using R&D Systems Recombinant Antibodies. We utilize our expertise in manufacturing high-quality antibodies and molecular biology to offer recombinant antibodies and antibody conversion and engineering services.

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What Are Recombinant Antibodies?

Recombinant antibodies are antibodies that are generated in vitro through inserting cloned genes into expression vectors. The expression vector is then inserted into a host cell to express the antibody. Our recombinant monoclonal antibody sequences are isolated from a pre-existing hybridoma, rabbit or llama antibody. By sequencing our antibodies and expressing them recombinantly we can provide an immortal supply with consistent results every time.

Rabbit monoclonal antibodiesTake advantage of the rabbit’s highly specific immune system with our Rabbit Monoclonal Antibodies.

Mouse/rat recombinant antibodies We have immortalized many of our hybridomas, so you can have a lifetime supply. See the full list of Mouse and Rat Recombinant Antibodies.

Advantages of Recombinant Antibody

By utilizing recombinant monoclonal antibodies, multiple advantages can be had over a traditional monoclonal or polyclonal antibody. These include being able to produce larger quantities, providing a long-term supply, and creating an antibody that provides reproducible results.


Increased Quantities

By developing and expressing recombinant antibodies in-house, we can meet all your size needs. We can produce antibodies in larger amounts such as 500 μg, 1 mg, and even into the gram quantities with a bulk recombinant antibody request.


Consistent and Reproducible Supply

When using recombinant antibodies, you can be assured of a long-term supply. All our antibodies are sequenced so we can guarantee consistency from lot to lot. By removing the variability found with traditional antibody production, you are guaranteed the same product time and time again.


Validated Antibodies

Our recombinant antibodies are held to the same high standard of validation testing as our traditional antibodies. We test every antibody we produce so you can remain confident in your results. Learn more about our antibody development and validation process.

 

Western blot detection of human P-Cadherin with a R&D Systems traditional hybridoma antibody.

Detection of Human P Cadherin by Western Blot with traditional hybridoma antibody. Western blot shows lysate of ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot detection of human P-Cadherin with a R&D Systems recombinant monoclonal antibody.

Detection of Human P Cadherin by Western Blot with recombinant antibody. Western blot shows lysates of ZR-75-1 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human P-Cadherin Monoclonal Antibody (Catalog # MAB861R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

 

Custom Antibody Engineering Services

Our expertise in converting hybridoma cell lines to recombinant antibodies makes us ideally suited to provide additional custom services. We can create custom recombinant antibodies that are engineered to reach your specific goals. Our recombinant antibody engineering services include:

  • Recombinant antibody conversions
  • Modification of the species, isotype, hinge, and Fc region
  • Generation of active truncated forms
  • Improved yield without any change in affinity

Discover All Custom Antibody Engineering Possibilities