Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 104805R
View all P-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human P-Cadherin
Asp108-Gly654
Accession # CAA45177

Specificity

Detects human P-Cadherin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human P‑Cadherin Antibody

Detection of Human P-Cadherin antibody by Western Blot.

Detection of Human P‑Cadherin by Western Blot.

Western blot shows lysates of ZR-75-1 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of P-Cadherin antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of P-Cadherin in A431 Human Cell Line by Flow Cytometry.

A431 human carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.
P-Cadherin antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

P‑Cadherin in MCF‑7 Human Cell Line.

P-Cadherin was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861R) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human P‑Cadherin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human cell line stained in a buffer containing Ca2+ and Mg2+

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: ZR‑75-1 human breast cancer cell line

Human P-Cadherin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human P‑Cadherin Biotinylated Antibody (Catalog # BAF861)
  • Standard: Recombinant Human P-Cadherin Fc Chimera Protein, CF (Catalog # 861-PC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB861R in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.

References

  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.

Long Name

Placental Cadherin

Alternate Names

CAD3, Cadherin-3, CDH3, CDHP, HJMD, PCAD, PCadherin

Entrez Gene IDs

1001 (Human); 12560 (Mouse); 116777 (Rat)

Gene Symbol

CDH3

UniProt

CAA45177

Additional P-Cadherin Products

Product Documents for Human P‑Cadherin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human P‑Cadherin Antibody

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

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  • Human P-Cadherin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: ZR-75-1 human breast cancer cell line and MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/08/2022
    Human P‑Cadherin Antibody MAB861R

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