|Detection of Human P‑Cadherin by Western Blot. Western blot shows lysate of ZR‑75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|P‑Cadherin in A431 Human Cell Line. P‑Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
Detection of Human P‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of ZR‑75 human breast cancer cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 135 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861) . This experiment was conducted under reducing conditions and using the|
12-230 kDa separation system.
Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.
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Flow Cytometry: Human P‑Cadherin Antibody [MAB861]
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