Human P-Cadherin Antibody

Catalog # Availability Size / Price Qty
MAB861-SP
MAB861-500
MAB861-100
Human P-Cadherin Antibody in Data
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human P-Cadherin Antibody Summary

Species Reactivity
Human
Specificity
Detects human P-Cadherin in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) Cadherin‑8, recombinant mouse P-Cadherin, or rhVE-Cadherin.
Source
Monoclonal Mouse IgG1 Clone # 104805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human P-Cadherin
Asp108-Gly654
Accession # CAA45177
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
P‑Cadherin is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in P‑Cadherin knockoutA431 cell line.
 

Human P-Cadherin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human P‑Cadherin Biotinylated Antibody (Catalog # BAF861)

Standard: Recombinant Human P-Cadherin Fc Chimera Protein, CF (Catalog # 861-PC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human P-Cadherin antibody by Western Blot. View Larger

Detection of Human P‑Cadherin by Western Blot. Western blot shows lysate of ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of P-Cadherin antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of P-Cadherin in A431 Human Cell Line by Flow Cytometry. A431 human carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry P-Cadherin antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

P‑Cadherin in A431 Human Cell Line. P-Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human P-Cadherin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human P‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of ZR‑75 human breast cancer cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 135 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human P-Cadherin Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human P‑Cadherin Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and P-Cadherin knockout A431 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated P-Cadherin Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

P-Cadherin Specificity is Shown by Flow Cytometry in Knockout Cell Line. P-Cadherin knockout A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). No staining in the P-Cadherin knockout A431 cell line was observed. Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

Knockout Validated P-Cadherin Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line. View Larger

P‑Cadherin Specificity is Shown by Immunocytochemistry in Knockout Cell Line. P-Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line, wildtype (left panel) but is not detected in P-Cadherin knockout (right panel), using Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane in wildtype cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Entrez Gene IDs
1001 (Human); 12560 (Mouse); 116777 (Rat)
Alternate Names
CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin

Product Datasheets

Citations for Human P-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation
    Authors: D Wang, NG Naydenov, MG Dozmorov, JE Koblinski, AI Ivanov
    Breast Cancer Res., 2020;22(1):3.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Cadherin-23 mediates heterotypic cell-cell adhesion between breast cancer epithelial cells and fibroblasts.
    Authors: Apostolopoulou M, Ligon L,
    PLoS ONE, 2012;7(3):e33289.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Development of anti-atherosclerotic tissue-engineered blood vessel by A20-regulated endothelial progenitor cells seeding decellularized vascular matrix.
    Authors: Zhu C, Ying D, Mi J, Li L, Zeng W, Hou C, Sun J, Yuan W, Wen C, Zhang W
    Biomaterials, 2008;29(17):2628-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human P-Cadherin Antibody

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Human P‑Cadherin Antibody
By Anonymous on 04/26/2016
Application: Flow Sample Tested: Cancer Cells Species: Human