Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 104805
View all P-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human P-Cadherin
Asp108-Gly654
Accession # CAA45177

Specificity

Detects human P-Cadherin in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) Cadherin‑8, recombinant mouse P-Cadherin, or rhVE-Cadherin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human P‑Cadherin Antibody

Detection of Human P-Cadherin antibody by Western Blot.

Detection of Human P‑Cadherin by Western Blot.

Western blot shows lysate of ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of P-Cadherin antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of P-Cadherin in A431 Human Cell Line by Flow Cytometry.

A431 human carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

P-Cadherin antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

P‑Cadherin in A431 Human Cell Line.

P-Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human P-Cadherin antibody by Simple WesternTM.

Detection of Human P‑Cadherin by Simple WesternTM.

Simple Western lane view shows lysates of ZR‑75 human breast cancer cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 135 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human P-Cadherin Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human P‑Cadherin Specificity by Using Knockout Cell Line.

Western blot shows lysates of A431 human epithelial carcinoma parental cell line and P-Cadherin knockout A431 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

P-Cadherin Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

P-Cadherin Specificity is Shown by Flow Cytometry in Knockout Cell Line.

P-Cadherin knockout A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). No staining in the P-Cadherin knockout A431 cell line was observed. Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

P-Cadherin Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line.

P‑Cadherin Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

P-Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line, wildtype (left panel) but is not detected in P-Cadherin knockout (right panel), using Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane in wildtype cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human P‑Cadherin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line stained in buffer containing Ca2+ and Mg2+

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line, wildtype and knockout

Knockout Validated

P‑Cadherin is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in P‑Cadherin knockoutA431 cell line.

Simple Western

10 µg/mL
Sample: ZR‑75 human breast cancer cell line and A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: ZR‑75 human breast cancer cell line

Human P-Cadherin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human P‑Cadherin Biotinylated Antibody (Catalog # BAF861)
  • Standard: Recombinant Human P-Cadherin Fc Chimera Protein, CF (Catalog # 861-PC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB861 in the following applications:

Flow Cytometry Panel Builder

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.

References

  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.

Long Name

Placental Cadherin

Alternate Names

CAD3, Cadherin-3, CDH3, CDHP, HJMD, PCAD, PCadherin

Entrez Gene IDs

1001 (Human); 12560 (Mouse); 116777 (Rat)

Gene Symbol

CDH3

UniProt

CAA45177

Additional P-Cadherin Products

Product Documents for Human P‑Cadherin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human P‑Cadherin Antibody

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Human P‑Cadherin Antibody

Customer Reviews for Human P‑Cadherin Antibody (2)

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  • Human P-Cadherin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 10/12/2021
    Human P‑Cadherin Antibody MAB861
  • Human P‑Cadherin Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Cancer Cells
    Species: Human
    Verified Customer | Posted 04/26/2016
    Human P‑Cadherin Antibody MAB861

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human P‑Cadherin Antibody

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  • Q: Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

    A: The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 

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