Recombinant Human IGF-I Protein, CF

(49 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.3-1.5 ng/mL.
    The specific activity of recombinant human IGF-l is approximately 2.5 IU/μg, which is calibrated against recombinant human IGF-l WHO International Standard (NIBSC code: 91/554).
  • Source
    E. coli-derived Gly49-Ala118
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly49
  • Predicted Molecular Mass
    7.6 kDa
  • SDS-PAGE
    7 kDa, reducing conditions
291-G1
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
1 μg/lane of Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is typically 0.3‑1.5 ng/mL.
Mass Spectrometry
MALDI-TOF analysis of Recombinant Human IGF-I.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.
Background: IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C‑terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF‑I is identical between isoforms and is generated by proteolytic removal of the N- and C‑terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4‑7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors (10).

  • References:
    1. Philippou, A. et al. (2007) In Vivo 21:45.
    2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
    3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
    4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
    5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
    6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
    7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
    8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
    9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
    10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  • Long Name:
    Insulin-like Growth Factor I
  • Entrez Gene IDs:
    3479 (Human); 16000 (Mouse); 24482 (Rat)
  • Alternate Names:
    IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Applications
Sample Type
  1. Specific activation of insulin-like growth factor-1 receptor by ginsenoside Rg5promotes angiogenesis and vasorelaxation.
    Authors: Cho Y, Hur S, Kim J, Kim J, Lee D, Choe J, Won M, Ha K, Jeoung D, Han S, Ryoo S, Lee H, Min J, Kwon Y, Kim D, Kim Y
    J Biol Chem, 2015;290(1):467-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015;125(3):1243-54.
    Species: Macaca nemistrina
    Sample Type: Whole Cells
    Application: Bioassay
  3. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
    Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
    Nat Commun, 2015;6(0):6576.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Insulin-like growth factor-I induces epithelial to mesenchymal transition via GSK-3beta and ZEB2 in the BGC-823 gastric cancer cell line.
    Authors: Li H, Xu L, Zhao L, Ma Y, Zhu Z, Liu Y, Qu X
    Oncol Lett, 2015;9(1):143-148.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  5. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2015;12(0):123.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway.
    Authors: Shen X, Zhang Y, Feng Y, Zhang L, Li J, Xie Y, Luo X
    Int J Oncol, 2014;44(3):791-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Selective and potent Akt inhibition triggers anti-myeloma activities and enhances fatal endoplasmic reticulum stress induced by proteasome inhibition.
    Authors: Mimura N, Hideshima T, Shimomura T, Suzuki R, Ohguchi H, Rizq O, Kikuchi S, Yoshida Y, Cottini F, Jakubikova J, Cirstea D, Gorgun G, Minami J, Tai Y, Richardson P, Utsugi T, Iwama A, Anderson K
    Cancer Res, 2014;74(16):4458-69.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Phosphoinositide protein kinase PDPK1 is a crucial cell signaling mediator in multiple myeloma.
    Authors: Chinen Y, Kuroda J, Shimura Y, Nagoshi H, Kiyota M, Yamamoto-Sugitani M, Mizutani S, Sakamoto N, Ri M, Kawata E, Kobayashi T, Matsumoto Y, Horiike S, Iida S, Taniwaki M
    Cancer Res, 2014;74(24):7418-29.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  9. Restoring Akt1 activity in outgrowth endothelial cells from South Asian men rescues vascular reparative potential.
    Authors: Cubbon R, Yuldasheva N, Viswambharan H, Mercer B, Baliga V, Stephen S, Askham J, Sukumar P, Skromna A, Mughal R, Walker A, Bruns A, Bailey M, Galloway S, Imrie H, Gage M, Rakobowchuk M, Li J, Porter K, Ponnambalam S, Wheatcroft S, Beech D, Kearney M
    Stem Cells, 2014;32(10):2714-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Circumvention of Mcl-1-dependent drug resistance by simultaneous Chk1 and MEK1/2 inhibition in human multiple myeloma cells.
    Authors: Pei X, Dai Y, Felthousen J, Chen S, Takabatake Y, Zhou L, Youssefian L, Sanderson M, Bodie W, Kramer L, Orlowski R, Grant S
    PLoS ONE, 2014;9(3):e89064.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
    Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
    Br J Cancer, 2014;110(12):2865-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Insulin signaling via Akt2 switches plakophilin 1 function from stabilizing cell adhesion to promoting cell proliferation.
    Authors: Wolf A, Rietscher K, Glass M, Huttelmaier S, Schutkowski M, Ihling C, Sinz A, Wingenfeld A, Mun A, Hatzfeld M
    J Cell Sci, 2013;126(0):1832-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2013;21(1):228-39.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  14. Protein distribution during human erythroblast enucleation in vitro.
    Authors: Bell A, Satchwell T, Heesom K, Hawley B, Kupzig S, Hazell M, Mushens R, Herman A, Toye A
    PLoS ONE, 2013;8(4):e60300.
    Species: Human
    Sample Type: cells
    Application: cell culture
  15. Role of IGF-1 pathway in lung fibroblast activation.
    Authors: Hung C, Rohani M, Lee S, Chen P, Schnapp L
    Respir Res, 2013;14(0):102.
    Species: Mouse
    Sample Type: Whole Cells
    Application: cell culture
  16. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2012;21(6):1321-39.
    Species: Human
    Sample Type: whole cells
    Application: cell culture
  17. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012;30(7):1455-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Authors: Zelinka C, Scott M, Volkov L, Fischer A
    PLoS ONE, 2012;7(9):e44477.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  19. The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems.
    Authors: Aad, Pauline, Echternkamp, Sherrill, Sypherd, David D, Schreiber, Nicole B, Spicer, Leon J
    Biol Reprod, 2012;87(4):79.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Bioassay
  20. Decorin antagonizes IGF receptor I (IGF-IR) function by interfering with IGF-IR activity and attenuating downstream signaling.
    Authors: Iozzo RV, Buraschi S, Genua M
    J. Biol. Chem., 2011;286(40):34712-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. IGFBP7 participates in the reciprocal interaction between acute lymphoblastic leukemia and BM stromal cells and in leukemia resistance to asparaginase.
    Authors: Laranjeira AB, de Vasconcellos JF, Sodek L, Spago MC, Fornazim MC, Tone LG, Brandalise SR, Nowill AE, Yunes JA
    Leukemia, 2011;26(5):1001-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Molecular pharmacology and antitumor activity of PHT-427, a novel Akt/phosphatidylinositide-dependent protein kinase 1 pleckstrin homology domain inhibitor.
    Authors: Meuillet EJ, Zuohe S, Lemos R, Ihle N, Kingston J, Watkins R, Moses SA, Zhang S, Du-Cuny L, Herbst R, Jacoby JJ, Zhou LL, Ahad AM, Mash EA, Kirkpatrick DL, Powis G
    Mol. Cancer Ther., 2010;9(3):706-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. A novel type of glial cell in the retina is stimulated by insulin-like growth factor 1 and may exacerbate damage to neurons and Muller glia.
    Authors: Fischer AJ, Scott MA, Zelinka C, Sherwood P
    Glia, 2010;58(6):633-49.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  24. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  25. Insulin-like growth factor-I receptor blockade by a specific tyrosine kinase inhibitor for human gastrointestinal carcinomas.
    Authors: Piao W, Wang Y, Adachi Y, Yamamoto H, Li R, Imsumran A, Li H, Maehata T, Ii M, Arimura Y, Lee CT, Shinomura Y, Carbone DP, Imai K
    Mol. Cancer Ther., 2008;7(6):1483-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Regulation of bone morphogenetic protein signalling in human pulmonary vascular development.
    Authors: Southwood M, Jeffery TK, Yang X, Upton PD, Hall SM, Atkinson C, Haworth SG, Stewart S, Reynolds PN, Long L, Trembath RC, Morrell NW
    J. Pathol., 2008;214(1):85-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Growth hormone promotes lymphangiogenesis.
    Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
    Am. J. Pathol., 2008;173(2):586-97.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
  28. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
    Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L
    Cell Stem Cell, 2008;2(6):602-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor.
    Authors: Spicer LJ, Aad PY
    Biol. Reprod., 2007;77(1):18-27.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Bioassay
  30. GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion.
    Authors: Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y
    Mol. Biol. Cell, 2007;18(10):3764-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  31. Pretreatment of adult bone marrow mesenchymal stem cells with cardiomyogenic growth factors and repair of the chronically infarcted myocardium.
    Authors: Bartunek J, Croissant JD, Wijns W, Gofflot S, de Lavareille A, Vanderheyden M, Kaluzhny Y, Mazouz N, Willemsen P, Penicka M, Mathieu M, Homsy C, De Bruyne B, McEntee K, Lee IW, Heyndrickx GR
    Am. J. Physiol. Heart Circ. Physiol., 2006;292(2):H1095-104.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  32. Growth differentiation factor-9 has divergent effects on proliferation and steroidogenesis of bovine granulosa cells.
    Authors: Spicer LJ, Aad PY, Allen D, Mazerbourg S, Hsueh AJ
    J. Endocrinol., 2006;189(2):329-39.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Bioassay
  33. Increased extraocular muscle strength with direct injection of insulin-like growth factor-I.
    Authors: Anderson BC, Christiansen SP, Grandt S, Grange RW, McLoon LK
    Invest. Ophthalmol. Vis. Sci., 2006;47(6):2461-7.
    Species: Rabbit
    Sample Type: In Vivo
    Application: In Vivo
  34. The effect of insulin-like growth factor-I on hepatocyte apoptosis after bile duct ligation in rat.
    Authors: Sheen-Chen SM, Ho HT, Chia-Pei L, Hung KS, Eng HL
    Dig. Dis. Sci., 2006;51(12):2220-4.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  35. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006;126(9):2096-105.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
    Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
    Glia, 2006;53(8):845-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice.
    Authors: Larsen PH, DaSilva AG, Conant K, Yong VW
    J. Neurosci., 2006;26(8):2207-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  38. Growth hormone-induced stimulation of multilineage human hematopoiesis.
    Authors: Hanley MB, Napolitano LA, McCune JM
    Stem Cells, 2005;23(8):1170-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  39. Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing.
    Authors: Brenner N, Huning M, Stange R
    Calcif. Tissue Int., 2004;74(4):388-97.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  40. Local delivery of growth factors from coated titanium plates increases osteotomy healing in rats.
    Authors: Wildemann B, Bamdad P, Holmer Ch, Haas NP, Raschke M, Schmidmaier G
    Bone, 2004;34(5):862-8.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  41. The effects of in ovo rhIGF-I administration on expression of the growth hormone secretagogue receptor (GHSR) during chicken embryonic development.
    Authors: Gahr SA, Kocamis H, Richter JJ, Killefer J
    Growth Dev Aging, 2004;68(1):3-10.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  42. Improvement of the developmental capacity of oocytes from prepubertal cattle by intraovarian insulin-like growth factor-I application.
    Authors: Oropeza A, Wrenzycki C, Herrmann D, Hadeler KG, Niemann H
    Biol. Reprod., 2004;70(6):1634-43.
    Species: Bovine
    Sample Type: In Vivo
    Application: In Vivo
  43. Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition.
    Authors: Studer RK, Bergman R, Stubbs T, Decker K
    Arthritis Res. Ther., 2004;6(1):R56-R64.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Bioassay
  44. Insulin growth factor-1 protects against excitotoxicity in the rat striatum.
    Authors: Escartin C, Boyer F, Bemelmans AP, Hantraye P, Brouillet E
    Neuroreport, 2004;15(14):2251-4.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  45. Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications.
    Authors: Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC
    Oncogene, 2002;21(37):5673-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  46. Detection of nitrotyrosine in aging and osteoarthritic cartilage: Correlation of oxidative damage with the presence of interleukin-1beta and with chondrocyte resistance to insulin-like growth factor 1.
    Authors: Loeser RF, Carlson CS, Del Carlo M, Cole A
    Arthritis Rheum., 2002;46(9):2349-57.
    Species: Primate – Macaca fascicularis (Cynomolgus/Crab-eating Monkey)
    Sample Type: Whole Cells
    Application: Bioassay
  47. Quantitative assessment of the integrity of the blood-retinal barrier in mice.
    Authors: Derevjanik NL, Vinores SA, Xiao WH, Mori K, Turon T, Hudish T, Dong S, Campochiaro PA
    Invest. Ophthalmol. Vis. Sci., 2002;43(7):2462-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  48. Reduction in the chondrocyte response to insulin-like growth factor 1 in aging and osteoarthritis: studies in a non-human primate model of naturally occurring disease.
    Authors: Loeser RF, Shanker G, Carlson CS, Gardin JF, Shelton BJ, Sonntag WE
    Arthritis Rheum., 2000;43(9):2110-20.
    Species: Primate – Macaca fascicularis (Cynomolgus/Crab-eating Monkey)
    Sample Type: Whole Cells
    Application: Bioassay
  49. Immediate early gene expression in dog thyrocytes in response to growth, proliferation, and differentiation stimuli.
    Authors: Deleu S, Pirson I, Clermont F, Nakamura T, Dumont JE, Maenhaut C
    J. Cell. Physiol., 1999;181(2):342-54.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
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Recombinant Human IGF-I, Animal-Free Protein

BA AFL291
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