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Recombinant Human IGF-I/IGF-1 Protein, CF

R&D Systems | Catalog # 291-G1

GMP Version Available: 291-GMP
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Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IGF-I/IGF-1 Protein (291-G1)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P05019

Applications

Bioactivity
View all IGF-I/IGF-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IGF-I/IGF-1 protein
Gly49-Ala118

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly49

Predicted Molecular Mass

7.6 kDa

SDS-PAGE

7 kDa, reducing conditions

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 0.3-1.5 ng/mL.

Reviewed Applications

Read 8 reviews rated 4.6 using 291-G1 in the following applications:

Scientific Data Images for Recombinant Human IGF-I/IGF-1 Protein, CF

Recombinant Human IGF-I/IGF-1 Protein SEC-MALS.

Recombinant Human IGF-I/IGF-1 Protein (Catalog # 291-G1) has a molecular weight (MW) of 7-8 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Graph showing dose-dependent bioactivity of IGF-1 protein

Bioactivity of Human IGF-1 Protein

Recombinant Human IGF-I / IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
Graph showing dose-dependent bioactivity of IGF-1 protein

Recombinant Human IGF-1 / IGF-1 Protein SDS-PAGE

1 μg/lane of Recombinant Human IGF-I / IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Graph showing dose-dependent bioactivity of IGF-1 protein

Bioactivity of Human IGF-1 Protein

MALDI-TOF analysis of Recombinant Human IGF-I / IGF-1. The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.

Formulation, Preparation, and Storage

291-G1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I/IGF-1

Insulin-like growth factor I (IGF-I or IGF-1), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C‑terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-1 protein is identical between isoforms and is generated by proteolytic removal of the N- and C‑terminal regions. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4‑7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). The IGF-I protein associates with IGF binding proteins thereby increasing its plasma half‑life and modulating its interactions with receptors (10).

References

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

P05019

Additional IGF-I/IGF-1 Products

Product Documents for Recombinant Human IGF-I/IGF-1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IGF-I/IGF-1 Protein, CF

For research use only

Citations for Recombinant Human IGF-I/IGF-1 Protein, CF

Customer Reviews for Recombinant Human IGF-I/IGF-1 Protein, CF (8)

4.6 out of 5
8 Customer Ratings
5 Stars
75%
4 Stars
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3 Stars
13%
2 Stars
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1 Stars
0%

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  • Recombinant Human IGF-I/IGF-1 Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 07/23/2021
  • Recombinant Human IGF-I/IGF-1 Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 05/14/2021
    Recombinant Human IGF-I/IGF-1 Protein, CF 291-G1
  • Recombinant Human IGF-I/IGF-1 Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 02/28/2021
  • Recombinant Human IGF-I/IGF-1 Protein, CF
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 01/09/2020
    Recombinant Human IGF-I/IGF-1 Protein, CF 291-G1
  • Recombinant Human IGF-I/IGF-1 Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/09/2019
    Recombinant Human IGF-I/IGF-1 Protein, CF 291-G1
  • Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 07/26/2018
  • Recombinant Human IGF-I Protein, CF
    Name: Balaji Mahender
    Application: Immunoassay Standard
    Verified Customer | Posted 02/26/2018
  • Recombinant Human IGF-I Protein, CF
    Name: Michelle Chen
    Application: Cell Proliferation
    Verified Customer | Posted 05/10/2017

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