Recombinant Human TLR4 Protein, CF
R&D Systems | Catalog # 1478-TR
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Key Product Details
- R&D Systems NS0-derived Recombinant Human TLR4 Protein (1478-TR)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TLR4 protein
Glu24-Lys631, with a C-terminal Ser and 10-His tag
Glu24-Lys631, with a C-terminal Ser and 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu24
Predicted Molecular Mass
70.6 kDa
SDS-PAGE
90-95 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human (rh) TLR4 is Immobilized at 2 μg/mL (100 μL/well), the concentration of rhMD2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 μg/mL.
When Recombinant Human (rh) TLR4 is Immobilized at 2 μg/mL (100 μL/well), the concentration of rhMD2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 μg/mL.
Reviewed Applications
Read 2 reviews rated 5 using 1478-TR in the following applications:
Formulation, Preparation, and Storage
1478-TR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: TLR4
References
- Miyake, K. (2004) Semin. Immunol. 16:11.
- Verstrepen, L. et al. (2008) Cell. Mol. Life Sci. 65:2964.
- Erridge, C. (2010) J. Leukoc. Biol. 87:989.
- Medzhitov, R. et al. (1997) Nature 388:394.
- Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
- Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. 98:12156.
- Akashi, S. et al. (2000) J. Immunol. 164:3471.
- Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
- Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
- Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
- Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. 101:4186.
- Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
- Pugin, J. et al. (2004) Blood 104:4071.
Long Name
Toll-like Receptor 4
Alternate Names
CD284
Gene Symbol
TLR4
UniProt
Additional TLR4 Products
Product Documents for Recombinant Human TLR4 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human TLR4 Protein, CF
For research use only
Citations for Recombinant Human TLR4 Protein, CF
Customer Reviews for Recombinant Human TLR4 Protein, CF (2)
5 out of 5
2 Customer Ratings
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 03/29/2021we use this protein to see interactions with TLR4 antibody, it has a very good affinity towards TLR4 monoclonal antibody in direct ELISA.
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 08/28/2020Different concentrations of human TLR4-His were used to determine the affinity an a TLR4-specific mAb using the Octet system.
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