Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF

Catalog #: 784-MR
1 Citation Datasheet / COA / SDS

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784-MR-050

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All Common gamma Chain/IL-2 R gamma Proteins and Enzymes

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Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF Summary

R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse Common gamma Chain Fc Chimera Protein (784-MR)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 2-10 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Common gamma Chain/IL-2 R gamma protein

Mouse Common gamma Chain (Ser25-Ala263) Accession # Q3UPA9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q3UPA9

N-terminal Sequence
Analysis

Ser25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

54.5 kDa (monomer)

SDS-PAGE

60 kDa, reducing conditions

Product Datasheets

784-MR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

784-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma is also designated the common gamma chain ( gamma _c) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Noguchi, M. et al. (1993) Science 262:1877.

Long Name

IL-2 Receptor gamma

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Alternate Names

CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; p64; SCIDX; SCIDX1; severe combined immunodeficiency

Related Research Areas

Citation for Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Potent antitumor activity of a designed interleukin-21 mimic
Authors: Chun, JH;Lim, BS;Roy, S;Walsh, MJ;Abhiraman, GC;Zhangxu, K;Atajanova, T;Revach, OY;Clark, EC;Li, P;Palin, CA;Khanna, A;Tower, S;Kureshi, R;Hoffman, MT;Sharova, T;Lawless, A;Cohen, S;Boland, GM;Nguyen, T;Peprah, F;Tello, JG;Liu, SY;Kim, CJ;Shin, H;Quijano-Rubio, A;Jude, KM;Gerben, S;Murray, A;Heine, P;DeWitt, M;Ulge, UY;Carter, L;King, NP;Silva, DA;Kueh, HY;Kalia, V;Sarkar, S;Jenkins, RW;Garcia, KC;Leonard, WJ;Dougan, M;Dougan, SK;Baker, D;
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