Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF

Name: Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 784-MR
Price: 454 USD
Availability: InStock

R&D Systems | Catalog # 784-MR

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse Common gamma Chain Fc Chimera Protein (784-MR)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q3UPA9

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all Common gamma Chain/IL-2 R gamma Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Common gamma Chain/IL-2 R gamma protein

Mouse Common gamma Chain (Ser25-Ala263) Accession # Q3UPA9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser25

Predicted Molecular Mass

54.5 kDa (monomer)

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells.
The ED₅₀ for this effect is 2-10 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.

Formulation, Preparation, and Storage

784-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma is also designated the common gamma chain ( gamma _c) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.