Mouse TGF-beta 2 DuoSet ELISA

R&D Systems | Catalog # DY7346-05

R&D Systems
100% Guarantee

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

7.81-500 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse TGF-beta 2 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all TGF-beta 2 ELISA Kits »

Product Summary for Mouse TGF-beta 2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse TGF-beta 2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse TGF-beta 2 DuoSet ELISA

Mouse TGF-beta 2 ELISA Standard Curve

Mouse TGF-beta 2 ELISA Standard Curve

Kit Contents for Mouse TGF-beta 2 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 2

TGF- beta 2 (transforming growth factor beta 2) is a highly pleiotropic cytokine with important functions during cardiac, lung, craniofacial, limb, eye, ear, and urogenital system development. It forms a latent complex with LAP and one of the LTBP TGF-beta binding proteins. The active protein is released by Plasmin, MMP, Thrombospondin 1, or Integrin action. TGF-beta 2 signals through TGF-beta RII, Betaglycan, and either TGF-beta RI/ALK-5 or ALK-1.

Long Name

Transforming Growth Factor beta 2

Alternate Names

TGFB2, TGFbeta 2

Entrez Gene IDs

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Gene Symbol

TGFB2

Additional TGF-beta 2 Products

Product Documents for Mouse TGF-beta 2 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TGF-beta 2 DuoSet ELISA

For research use only

Citations for Mouse TGF-beta 2 DuoSet ELISA

Customer Reviews for Mouse TGF-beta 2 DuoSet ELISA (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse TGF-beta 2 DuoSet ELISA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Mouse TGF-beta 2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 04/01/2019
    I tested mouse serum using acid activation and a 1:4 diluted. I mouse detectable levels in all 5 samples I used. I did not observe any cross reactivity with TGF beta 1 or 3.
    Mouse TGF-beta 2 DuoSet ELISA DY7346-05

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits