STAT3 Antibody (24GB5510)

Name: STAT3 Antibody (24GB5510)
Brand: Novus Biologicals
SKU: NBP3-51721
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-51721

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 24GB5510

View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human STAT3 (Uniprot: P40763)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Scientific Data Images for STAT3 Antibody (24GB5510)

Immunocytochemistry/ Immunofluorescence: STAT3 Antibody (24GB5510)[NBP3-51721] -

Immunocytochemical staining of HepG2 cells with STAT3 antibody (1:1,000). Nuclei were stained blue with DAPI; STAT3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 um.

Flow Cytometry: STAT3 Antibody (24GB5510)[NBP3-51721] -

Flow cytometric analysis of STAT3expression in HepG2 cells using STAT3 antibody (1:2,000). Green, isotype control; red, STAT3.

Flow Cytometry: STAT3 Antibody (24GB5510)[NBP3-51721] -

Validation of STAT3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with STAT3 antibody (1:2,000) and analyzed using BD flow cytometer.

Western Blot: STAT3 Antibody (24GB5510)[NBP3-51721] -

Western blotting analysis using STAT3 antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Western Blot: STAT3 Antibody (24GB5510)[NBP3-51721] -

Western blotting analysis using STAT3 antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit(signal transducer and activator of transcription 3.

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Applications for STAT3 Antibody (24GB5510)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

Additional STAT3 Products

Product Documents for STAT3 Antibody (24GB5510)

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Certificate of Analysis

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Product Specific Notices for STAT3 Antibody (24GB5510)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.