STAT3 Antibody - BSA Free

Name: STAT3 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-38857
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-38857

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit STAT3 Antibody - BSA Free (NBP2-38857) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT3 Antibody - BSA Free

Western Blot: STAT3 Antibody [NBP2-38857]

Western Blot: STAT3 Antibody [NBP2-38857] - Analysis in human cell lines A-549 and PC-3 using anti-STAT3 antibody. Corresponding STAT3 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857]

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857] - Staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857]

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857] - Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857]

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857] - Staining of human fallopian tube shows moderate cytoplasmic and nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857]

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857]

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP2-38857] - Staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.

Chromatin Immunoprecipitation-exo-Seq: STAT3 Antibody - BSA Free [NBP2-38857]

ChIP-Exo-Seq composite graph for Anti-STAT3 (NBP2-38857) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

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Applications for STAT3 Antibody - BSA Free

Application

Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

UniProt

P40763

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Product Specific Notices for STAT3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.