STAT5A [p Tyr694] Antibody (3F1) - BSA Free

Name: STAT5A [p Tyr694] Antibody (3F1) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26387
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26387

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3F1 expressed in HEK293

Format

BSA Free

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Product Specifications

Immunogen

A synthesized peptide derived from Human STAT5A [p Tyr694] [UniProt P42229]

Modification

p Tyr 694

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAT5A [p Tyr694] Antibody (3F1) - BSA Free

Immunohistochemistry: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] -

Immunohistochemistry: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] - Image of STAT5A [p Tyr694] Antibody (3F1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] -

Western Blot: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] - Positive Western Blot detected in HepG2 whole cell lysate (treated with EGF or not).
All lanes: STAT5A [p Tyr694] Antibody at 1.025ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 90 KDa
Observed band size: 90 KDa

Applications for STAT5A [p Tyr694] Antibody (3F1) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: STAT5a

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Gene Symbol

STAT5A

Additional STAT5a Products

Product Documents for STAT5A [p Tyr694] Antibody (3F1) - BSA Free

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Product Specific Notices for STAT5A [p Tyr694] Antibody (3F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.