STAT5A Antibody (6D4) - BSA Free

Name: STAT5A Antibody (6D4) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37441
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37441

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 6D4

Format

BSA Free

View all STAT5a Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human STAT5A (AA: 583-794) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

90.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse STAT5A Antibody (6D4) - BSA Free (NBP2-37441) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT5A Antibody (6D4) - BSA Free

Western Blot: STAT5A Antibody (6D4)BSA Free [NBP2-37441]

Western Blot: STAT5a Antibody (6D4) [NBP2-37441] - Western blot analysis using STAT5A mouse mAb against K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate.

Immunocytochemistry/ Immunofluorescence: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Immunocytochemistry/Immunofluorescence: STAT5a Antibody (6D4) [NBP2-37441] - Immunofluorescence analysis of Hela cells using STAT5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Immunohistochemistry: STAT5a Antibody (6D4) [NBP2-37441] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using STAT5A mouse mAb with DAB staining.

Flow Cytometry: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Flow Cytometry: STAT5a Antibody (6D4) [NBP2-37441] - Flow cytometric analysis of K562 cells using STAT5A mouse mAb (green) and negative control (red).

Western Blot: STAT5A Antibody (6D4)BSA Free [NBP2-37441]

Western Blot: STAT5a Antibody (6D4) [NBP2-37441] - Western blot analysis using STAT5A mAb against human STAT5A recombinant protein. (Expected MW is 49.3 kDa)

Immunohistochemistry: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Immunohistochemistry: STAT5a Antibody (6D4) [NBP2-37441] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using STAT5A mouse mAb with DAB staining.

ELISA: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

ELISA: STAT5a Antibody (6D4) [NBP2-37441] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

View 4 more images

Applications for STAT5A Antibody (6D4) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Application Notes

This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT5a

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Gene Symbol

STAT5A

Additional STAT5a Products

Product Documents for STAT5A Antibody (6D4) - BSA Free

Download Product Datasheets

Download SDS

Product Specific Notices for STAT5A Antibody (6D4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.