STAT5A Antibody (OTI9F7)

Name: STAT5A Antibody (OTI9F7)
Brand: Novus Biologicals
SKU: NBP2-00622
Price: 459 USD
Availability: InStock
Rating: 4 (2 reviews)

Novus Biologicals | Catalog # NBP2-00622

(2)

Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI9F7

View all STAT5a Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

90.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse STAT5A Antibody (OTI9F7) (NBP2-00622) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-STAT5A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT5A Antibody (OTI9F7)

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622]

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of extracts (35ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human prostate tissue using anti-STAT5A mouse monoclonal antibody.

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622]

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Jurkat cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622]

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymph node tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymphoma tissue using anti-STAT5A mouse monoclonal antibody.

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622]

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Hela cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

View 8 more images

Applications for STAT5A Antibody (OTI9F7)

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Reviewed Applications

Read 2 reviews rated 4 using NBP2-00622 in the following applications:

Simple Western (2 Reviews)

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.73 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT5a

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Entrez Gene IDs

6776 (Human)

Gene Symbol

STAT5A

Additional STAT5a Products

Product Documents for STAT5A Antibody (OTI9F7)

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Product Specific Notices for STAT5A Antibody (OTI9F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Transcription Factors and Regulators

Hematopoietic Stem Cell Markers

Interferons

PDGF Family

Regulatory T Cells (Tregs)

Transcription Factors in the Akt Pathway

Citations for STAT5A Antibody (OTI9F7)

Customer Reviews for STAT5A Antibody (OTI9F7) (2)

4 out of 5

2 Customer Ratings

5 Stars

50%

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50%

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STAT5a Antibody (9F7)

Name: Rognvald Blance

Application: Simple Western

Sample Tested: K562 human chronic myelogenous leukemia cell line

Species: Human

Verified Customer | Posted 03/22/2017
STAT5a Antibody (9F7)

Name: Rognvald Blance

Application: Simple Western

Sample Tested: K562 human chronic myelogenous leukemia cell line

Species: Human

Verified Customer | Posted 02/06/2017

Sta5a

Cells used = K562 or K562 + imatinib - lysed in Protein Simple bicine/CHAPS buffer (040-764) loaded at 100 ng/µL; Gradient = 5 – 8 nested, ladder# 3; Primary antibody at 1:50; Secondary antibody = Protein Simple biotinylated anti-mouse (041-127) with Protein Simple streptavidin-HRP (041-106) both at 1:100.