Streptavidin Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Streptavidin by Western Blot.
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Product Details
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Streptavidin Antibody Summary

Detects Streptavidin in Western blot.
Recombinant Monoclonal Rabbit IgG Clone # 1220C
Protein A or G purified from cell culture supernatant
Purified streptavidin from Streptomyces avidinii
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Streptavidin antibody by Western Blot. View Larger

Detection of Streptavidin by Western Blot. Western blot shows recombinant Streptavidin. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Streptavidin Monoclonal Antibody (Catalog # MAB9020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Streptavidin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Streptavidin

Streptavidin is a 52.8 kDa protein from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetramer forming a beta-barrel with an extraordinarily high affinity for biotin (also known as vitamin B7). It has a dissociation constant (Kd) on the order of ≈10−14 mol/L. The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature and has a high resistance to organic solvents, denaturants, detergents, proteolytic enzymes, and extreme pH ranges or temperatures.

Alternate Names
SA V1; SA V2; Streptavidin V1; Streptavidin V2; Streptavidin

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