Detection of Streptavidin by Western Blot. Western blot shows recombinant Streptavidin. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Streptavidin Monoclonal Antibody (Catalog # MAB9020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Streptavidin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Streptavidin is a 52.8 kDa protein from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetramer forming a beta-barrel with an extraordinarily high affinity for biotin (also known as vitamin B7). It has a dissociation constant (Kd) on the order of ≈10−14 mol/L. The binding of biotin to streptavidin is one of the strongest
non-covalent interactions known in nature and has a high resistance to organic solvents,
denaturants, detergents, proteolytic enzymes, and extreme pH ranges or temperatures.
SA V1; SA V2; Streptavidin V1; Streptavidin V2; Streptavidin
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