Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1220C
View all Streptavidin Primary Antibodies »

Product Specifications

Immunogen

Purified streptavidin from Streptomyces avidinii

Specificity

Detects Streptavidin in Western blot.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Streptavidin Antibody

Detection of Streptavidin antibody by Western Blot.

Detection of Streptavidin by Western Blot.

Western blot shows recombinant Streptavidin. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Streptavidin Monoclonal Antibody (Catalog # MAB9020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Streptavidin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Streptavidin Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Recombinant streptavidin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Streptavidin

Streptavidin is a 52.8 kDa protein from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetramer forming a beta-barrel with an extraordinarily high affinity for biotin (also known as vitamin B7). It has a dissociation constant (Kd) on the order of ≈10−14 mol/L. https://en.wikipedia.org/wiki/Streptavidin#cite_note-1">The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature and has a high resistance to organic solvents, denaturants, detergents, proteolytic enzymes, and extreme pH ranges or temperatures.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

Additional Streptavidin Products

Product Documents for Streptavidin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Streptavidin Antibody

For research use only

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