Streptavidin-HRP (set of 5 vials enough for 75 plates total) DY998: R&D Systems

INTENDED USE & DESCRIPTION

Streptavidin-HRP (Part # 890803) contains 5 vials (1 mL/vial) of streptavidin conjugated to horseradish peroxidase for use with biotinylated detection antibodies in ELISAs and Western Blotting.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 6 months after opening when stored at 2-8 °C. Do Not Freeze.

REAGENT PREPARATION

For use in ELISAs, dilute to the working concentration specified on the vial label using 1% BSA/PBS or Reagent Diluent Concentrate 2 (Catalog # DY995) diluted 1:10 in deionized water. For use in Western blots, the suggested dilution is 1:200-1:2000 in an appropriate diluent. Use the diluted reagent within 60 minutes. 

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
    Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
    PLoS ONE, 2017;12(1):e0168565. 2017
  2. A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
    Authors: DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson
    PLoS ONE, 2017;12(4):e0174447. 2017
  3. A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1
    Genes Dev, 2016;30(9):1070-85. 2016
  4. Naturally Occurring Autoantibodies against Tau Protein Are Reduced in Parkinson's Disease Dementia
    PLoS ONE, 2016;11(11):e0164953. 2016
  5. Defects in Stratum Corneum Desquamation Are the Predominant Effect of Impaired ABCA12 Function in a Novel Mouse Model of Harlequin Ichthyosis
    PLoS ONE, 2016;11(8):e0161465. 2016
  6. Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice
    Authors: T Nakano, R Kamei, T Fujimura, Y Takaoka, A Hori, CY Lai, KC Chiang, Y Shimada, N Ohmori, T Goto, K Ono, CL Chen, S Goto, S Kawamoto
    PLoS ONE, 2016;11(4):e0153630. 2016
  7. Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge
    PLoS ONE, 2016;11(8):e0161169. 2016
  8. TLR4 and TLR7/8 Adjuvant Combinations Generate Different Vaccine Antigen-Specific Immune Outcomes in Minipigs when Administered via the ID or IN Routes.
    Authors: McKay P, King D, Mann J, Barinaga G, Carter D, Shattock R
    PLoS ONE, 2016;11(2):e0148984. 2016
  9. Upregulated interleukin-21 receptor on B cells associated with the downregulation of IgE in patients with allergic rhinitis.
    Authors: Jen, Hsiao-Yu, Yang, Yao-Hsu, Chiang, Bor-Luen, Chuang, Ya-Hui
    J Interferon Cytokine Res, 2015;35(1):42-8. 2015
  10. The effects of two polymorphisms on p21cip1 function and their association with Alzheimer's disease in a population of European descent.
    Authors: Yates, Sharon C, Zafar, Amen, Rabai, Erzsebet, Foxall, James B, Nagy, Sheila, Morrison, Karen E, Clarke, Carl, Esiri, Margaret, Christie, Sharon, Smith, A David, Nagy, Zsuzsann
    PLoS ONE, 2015;10(1):e0114050. 2015
  11. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772. 2015
  12. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
    Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
    J Neuroinflammation, 2015;12(0):119. 2015
  13. Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
    Authors: Lee W, Shen S, Shih Y, Chou C, Tseng J, Chin S, Liu K, Chen Y, Jiang M
    J Transl Med, 2015;13(0):191. 2015
  14. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
    Authors: Costa-Pereira C, Moreira M, Soares R, Marteleto B, Ribeiro V, Franca-Dias M, Cardoso L, Viana K, Giunchetti R, Martins-Filho O, Araujo M
    BMC Vet Res, 2015;11(0):92. 2015
  15. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015;6(0):6224. 2015
  16. Glucopyranosyl lipid A adjuvant significantly enhances HIV specific T and B cell responses elicited by a DNA-MVA-protein vaccine regimen.
    Authors: McKay P, Cope A, Mann J, Joseph S, Esteban M, Tatoud R, Carter D, Reed S, Weber J, Shattock R
    PLoS ONE, 2014;9(1):e84707. 2014
  17. Genome-wide identification and quantification of protein synthesis in cultured cells and whole tissues by puromycin-associated nascent chain proteomics (PUNCH-P).
    Authors: Aviner, Ranen, Geiger, Tamar, Elroy-Stein, Orna
    Nat Protoc, 2014;9(4):751-60. 2014
  18. Distinct patterns of IgG and IgA against food and microbial antigens in serum and feces of patients with inflammatory bowel diseases.
    Authors: Frehn L, Jansen A, Bennek E, Mandic A, Temizel I, Tischendorf S, Verdier J, Tacke F, Streetz K, Trautwein C, Sellge G
    PLoS ONE, 2014;9(9):e106750. 2014
  19. Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis.
    Authors: Moelants, Eva A V, Loozen, Gitte, Mortier, Anneleen, Martens, Erik, Opdenakker, Ghislain, Mizgalska, Danuta, Szmigielski, Borys, Potempa, Jan, Van Damme, Jo, Teughels, Wim, Proost, Paul
    Infect Immun, 2014;82(6):2511-9. 2014
  20. Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.
    Authors: Rossi O, Pesce I, Giannelli C, Aprea S, Caboni M, Citiulo F, Valentini S, Ferlenghi I, MacLennan C, D'Oro U, Saul A, Gerke C
    J Biol Chem, 2014;289(36):24922-35. 2014
  21. Proteome-wide screening reveals immunodominance in the CD8 T cell response against classical swine fever virus with antigen-specificity dependent on MHC class I haplotype expression.
    Authors: Franzoni G, Kurkure N, Essler S, Pedrera M, Everett H, Bodman-Smith K, Crooke H, Graham S
    PLoS ONE, 2013;8(12):e84246. 2013
  22. A "prime-pull" vaccine strategy has a modest effect on local and systemic antibody responses to HIV gp140 in mice.
    Authors: Tregoning, John S, Buffa, Viviana, Oszmiana, Anna, Klein, Katja, Walters, Adam A, Shattock, Robin J
    PLoS ONE, 2013;8(11):e80559. 2013
  23. Administration of a Toll-like receptor 9 agonist decreases the proviral reservoir in virologically suppressed HIV-infected patients.
    Authors: Winckelmann A, Munk-Petersen L, Rasmussen T, Melchjorsen J, Hjelholt T, Montefiori D, Ostergaard L, Sogaard O, Tolstrup M
    PLoS ONE, 2013;8(4):e62074. 2013
  24. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036. 2012
  25. Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
    J. Immunol., 2011;186(8):4967-72. 2011
  26. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011;19(10):1793-801. 2011
  27. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011;186(4):2613-22. 2011
  28. Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
    Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
    Neuropharmacology, 2010;59(7):640-9. 2010
  29. Expression of type III interferon (IFN) in the vaginal mucosa is mediated primarily by dendritic cells and displays stronger dependence on NF-kappaB than type I IFNs.
    Authors: Iversen MB, Ank N, Melchjorsen J, Paludan SR
    J. Virol., 2010;84(9):4579-86. 2010
  30. Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
    Authors: Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL
    Cancer Lett., 2010;290(1):49-57. 2010
  31. Tumoural expression and circulating level of VEGFR-3 (Flt-4) in metastatic melanoma patients: correlation with clinical parameters and outcome.
    Authors: Mouawad R, Spano JP, Comperat E, Capron F, Khayat D
    Eur. J. Cancer, 2009;45(8):1407-14. 2009
  32. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400. 2009
  33. Facilitated in vivo synthesis of ribonucleic acid and protein via T7 RNA polymerase.
    Authors: Wang TH, Yu SH, Au LC
    Anal. Biochem., 2008;375(1):97-104. 2008
  34. Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
    Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
    Clin. Exp. Immunol., 2008;152(2):328-35. 2008
  35. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41. 2008
  36. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007;132(1):58-63. 2007
  37. Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
    Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
    Blood, 2007;110(6):1748-55. 2007
  38. Differential gene expression in cultured osteoblasts and bone marrow stromal cells from patients with Paget's disease of bone.
    Authors: Naot D, Bava U, Matthews B, Callon KE, Gamble GD, Black M, Song S, Pitto RP, Cundy T, Cornish J, Reid IR
    J. Bone Miner. Res., 2007;22(2):298-309. 2007
  39. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007;26(4):445-59. 2007
  40. Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes.
    Authors: Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE
    J. Immunol., 2007;179(12):8381-91. 2007
  41. Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
    Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
    Blood, 2007;111(3):1021-8. 2007
  42. Expression and regulation of the orphan receptor RDC1 and its putative ligand in human dendritic and B cells.
    Authors: Infantino S, Moepps B, Thelen M
    J. Immunol., 2006;176(4):2197-207. 2006
  43. Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal.
    Authors: Greber B, Lehrach H, Adjaye J
    Stem Cells, 2006;25(2):455-64. 2006
  44. Serotonin and fluoxetine modulate bone cell function in vitro.
    Authors: Gustafsson BI, Thommesen L, Stunes AK, Tommeras K, Westbroek I, Waldum HL, Slordahl K, Tamburstuen MV, Reseland JE, Syversen U
    J. Cell. Biochem., 2006;98(1):139-51. 2006
Expand to show all 44 Citations
Supplemental ELISA Products
Description Application Cat# Citations Images  

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)

ELISA DY999 57
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Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)

ELISA DY995 17
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Stop Solution 2N Sulfuric Acid (15 x 6 mL)

ELISA DY994 12
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ELISA Plate Sealers, 100 Pack

ELISA DY992
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Quantikine Wash Buffer 1

ELISA WA126 6
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Clear Polystyrene Microplates, 25 Pack

ELISA DY990 3
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Reagent Additive 1 (21 mL)

ELISA DY005 1
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Glo Substrate Reagent Pack (Reagent A & B)

ELISA DY993 3
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Cell Lysis Buffer 2 (1 x 21 mL)

ELISA 895347 2
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Reagent Diluent Concentrate 1 (21 mL)

ELISA DY997 2
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Sample Diluent Concentrate 2 (2X)

ELISA DYC002 2
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Reagent Diluent Concentrate 3 (5X, 10 x 21 mL)

ELISA DY004
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Sample Diluent Concentrate 1 (5X, 5 x 21 mL)

ELISA DYC001 1
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Cell Lysis Buffer 1 (1 x 21 mL)

ELISA 890713 1
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Black Polystyrene Microplates, 25 Pack

ELISA DY991 2
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Cell Lysis Buffer 5 (1 x 21 mL)

ELISA 895890 1
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Cell Lysis Buffer 3 Concentrate (5X, 1 x 21 mL)

ELISA 895366
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Average Rating: 4.3 (Based on 3 reviews)

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 Streptavidin-HRP (set of 5 vials enough for 75 plates total) DY998
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Excellent
  Anonymous 07/20/2017
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 Streptavidin-HRP (set of 5 vials enough for 75 plates total) DY998
: Streptavidin-HRP (set of 5 vials enough for 75 plates total) [DY998]

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Other Experimental DetailsStandard ELISA protocol observed for concentration analysis
  Very Good
  Anonymous 12/21/2016
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Other Experimental Details

Other Experimental DetailsWestern blot of biotinated molecule.1:1000dilution of the product in BSA
  Very Good
  Anonymous 06/02/2016
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