Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

Name: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37282
Price: 199 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37282

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1A3H4

Format

BSA Free

View all Syndecan-1/CD138 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Syndecan-1/CD138 (AA: 28-171) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

32.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

Western Blot: Syndecan-1/CD138 Antibody (1A3H4)BSA Free [NBP2-37282]

Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mouse mAb against MCF-7 (1), Hela (2), HepG2 (3), T47D (4), SW620 (5), Jurkat (6) and NIH/3T3 (7) cell lysate.

Immunocytochemistry/ Immunofluorescence: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of rectum cancer tissues using SDC1 mouse mAb with DAB staining.

Flow Cytometry: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Flow Cytometry: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of HepG2 cells using SDC1 mouse mAb (green) and negative control (red).

Western Blot: Syndecan-1/CD138 Antibody (1A3H4)BSA Free [NBP2-37282]

Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mAb against human SDC1 (AA: 28-171) recombinant protein. (Expected MW is 44.4 kDa)

Western Blot: Syndecan-1/CD138 Antibody (1A3H4)BSA Free [NBP2-37282]

Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mAb against HEK293 (1) and SDC1 (AA: 28-171)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of ovarian cancer tissues using SDC1 mouse mAb with DAB staining.

ELISA: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

ELISA: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

View 5 more images

Applications for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Simple Western

1:10

Western Blot

1:500 - 1:2000

Application Notes

This antibody is Cytof ready.
See Simple Western Antibody Database for Simple Western validation: Tested in 12Z, Ovaries, separated by Size, antibody dilution of 1:10, apparent MW was 65 kDa

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Advanced Features

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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-1/CD138

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Alternate Names

CD138, SDC1, Syndecan1

Gene Symbol

SDC1

Additional Syndecan-1/CD138 Products

Product Documents for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

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Certificate of Analysis

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Product Specific Notices for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.