Tankyrase 1 Activity Assays

The Tankyrase 1 Activity Assays are high-throughput, semi-quantitative ELISAs designed to measure Tankyrase 1 activity. Tankyrase 1, also known as PARP5a, is a Poly (ADP-ribose) Polymerase (PARP) that associates with telomeres via its interaction with the telomeric-repeat binding factor TRF1. These assays utilize a histone-coated 96-well microplate and an anti-poly (ADP-ribose) (PAR) antibody to detect PAR that has been deposited onto the immobilized histone proteins by Tankyrase 1. Assays are available in a colorimetric detection format, which uses TACS-Sapphire™, or a chemiluminescent detection format, which uses PARP PeroxyGlow™ A and B. Additionally, these are the only commercially available assays to feature a full length Tankyrase 1 enzyme and unmodified NAD substrate to create a more physiologically applicable in vitro assay system.

Available Kits


  • Two non-radioactive detection formats available
  • Amenable to high-throughput
  • Highly sensitive: capable of measuring 0.1 mUnits of Tankyrase 1
  • 3 hour assay time
  • Provides full length Tankyrase 1 enzyme and unmodified NAD substrate

Kit Contents

  • Histone-coated 96-stripwell Microplate
  • Anti-PAR Monoclonal Antibody
  • HRP-conjugated Goat Anti-Mouse Antibody
  • Tankyrase 1
  • XAV939a
  • Diluents & Buffers
  • Unmodified NAD Substrate
  • HRP Substrate

Data Example

Inhibition of Tankyrase 1 Activity by XAV939

Graphical representation of a chemiluminescent Tankyrase 1 standard curve (A) and an inhibition curve for the Tankyrase 1 inhibitor XAV939 (provided in the kit; B). The standard curve is used to translate absorbance values to activity units of Tankyrase 1.

aXAV939 is a Tankyrase 1 inhibitor