TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74495

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E12

Format

Azide and BSA Free
View all TGF-beta 1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TGFB1(NP_000651) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

28.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Western Blot: TGF-beta 1 Antibody (OTI1E12)Azide and BSA Free [NBP2-74495]

Western Blot: TGF-beta 1 Antibody (OTI1E12)Azide and BSA Free [NBP2-74495]

Western Blot: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGR-beta 1.
Immunocytochemistry/ Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Immunocytochemistry/ Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Immunocytochemistry/Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TGF-Beta 1.
Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495] - Analysis of SF295 cells, using TGFB1 antibody,(Red), compared to a nonspecific negative control antibody (Blue).
Immunocytochemistry/ Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Immunocytochemistry/ Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Immunocytochemistry/Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495] - Analysis of HeLa cells.

Applications for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 1

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

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