TGF-beta 1, 2, 3 Antibody

(108 citations)
(2 Reviews)
  
  • Specificity
    Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
  • Source
    Monoclonal Mouse IgG1 Clone # 1D11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Bovine bone-derived TGF-beta 1 and TGF-beta 2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TGF-beta 1 (Catalog # 240-B) under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
    • Human TGF-beta 1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    TGF-beta 1, 2, 3 Antibody (Catalog # MAB1835)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    TGF‑ beta 1 Biotinylated Antibody (Catalog # BAF240)
  • ELISA Standard
     
    Recombinant Human TGF-beta 1 Protein (Catalog # 240-B)
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1990) Lymphokine Res. 9:607. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.
  • Binding Inhibition
    Dasch, J.R. et al. (1994) J. Immunol. 142:1536.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TGF‑ beta in PC-3 Human Cell Line. TGF‑ beta 1, 2, 3 was detected in immersion fixed PC-3 human prostate cancer cell line using 10 µg/mL Mouse Anti-TGF‑ beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
TGF-beta in Human Skin. TGF-beta 1, 2, 3 was detected in immersion fixed paraffin-embedded human skin using 25 µg/mL Mouse
Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835) overnight at 4 °C. Tissues was stained using the Anti-Mouse HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with Haematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1, 2, 3 Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240‑B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‑ beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835). The ND50 is typically
0.25‑1.25 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 1, 2, 3
TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
  • References:
    1. Ayala A. et al. (1992) FASEB J. 6:A1604.
    2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
    3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.
  • Long Name:
    Transforming Growth Factor beta 1, 2, and 3
  • Alternate Names:
    CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

108 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3(+) regulatory T cells
    Authors: M Pasztoi, J Pezoldt, M Beckstette, C Lipps, D Wirth, M Rohde, K Paloczi, EI Buzas, J Huehn
    Eur. J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. ALS skeletal muscle shows enhanced TGF-? signaling, fibrosis and induction of fibro/adipogenic progenitor markers
    Authors: D Gonzalez, O Contreras, DL Rebolledo, JP Espinoza, B van Zunder, E Brandan
    PLoS ONE, 2017;12(5):e0177649.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function
    Authors: RM Petri, A Hackel, K Hahnel, CA Dumitru, K Bruderek, SB Flohe, A Paschen, S Lang, S Brandau
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Integrin ?1 activation induces an anti-melanoma host response
    Authors: L Ritsma, I Dey-Guha, N Talele, X Sole, Salony, J Chowdhury, KN Ross, S Ramaswamy
    PLoS ONE, 2017;12(4):e0175300.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  5. The therapeutic HIV Env C5/gp41 vaccine candidate Vacc-C5 induces specific T cell regulation in a phase I/II clinical study
    Authors: K Brekke, M Sommerfelt, M Ökvist, AM Dyrhol-Rii, D Kvale
    BMC Infect. Dis, 2017;17(1):228.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. TGF-? Neutralization Enhances AngII-Induced Aortic Rupture and Aneurysm in Both Thoracic and Abdominal Regions
    Authors: X Chen, DL Rateri, DA Howatt, A Balakrishn, JJ Moorleghen, LA Cassis, A Daugherty
    PLoS ONE, 2016;11(4):e0153811.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. Apoptotic cell infusion treats ongoing collagen-induced arthritis, even in the presence of methotrexate, and is synergic with anti-TNF therapy
    Arthritis Res Ther, 2016;18(1):184.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  8. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016;11(11):e0167410.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion
    PLoS ONE, 2016;11(11):e0166500.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Mammary epithelial cell phagocytosis downstream of TGF-beta3 is characterized by adherens junction reorganization.
    Authors: Fornetti J, Flanders K, Henson P, Tan A, Borges V, Schedin P
    Cell Death Differ, 2016;23(2):185-96.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  11. [64Cu]XYIMSR-06: A dual-motif CAIX ligand for PET imaging of clear cell renal cell carcinoma
    Authors: I Minn, SM Koo, HS Lee, M Brummet, SP Rowe, MA Gorin, P Sysa-Shah, WD Lewis, HH Ahn, Y Wang, SR Banerjee, RC Mease, S Nimmagadda, ME Allaf, MG Pomper, X Yang
    Oncotarget, 2016;7(35):56471-56479.
    Species: Mink
    Sample Type: Whole Cells
    Application: Neut
  12. Different tissue phagocytes sample apoptotic cells to direct distinct homeostasis programs
    Nature, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. K+/Cl- cotransporter KCC2 membrane trafficking and functionality is regulated by transforming growth factor beta 2
    J Cell Sci, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-? signalling
    Authors: Fatemeh Mirzamoham
    Nat Commun, 2016;7(0):12047.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  15. TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development
    Authors: H Nagashima, Y Okuyama, T Hayashi, N Ishii, T So
    J Immunol, 2016;0(0):.
  16. TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
    PLoS ONE, 2016;11(9):e0162221.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Sterile Inflammation Enhances ECM Degradation in Integrin ?1 KO Embryonic Skin
    Cell Rep, 2016;16(12):3334-47.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  18. Autonomous and Non-autonomous Defects Underlie Hypertrophic Cardiomyopathy in BRAF-Mutant hiPSC-Derived Cardiomyocytes
    Stem Cell Reports, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  19. Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-beta (TGF-beta) Activation and Fibroblast Differentiation.
    Authors: Dayer C, Stamenkovic I
    J Biol Chem, 2015;290(22):13763-78.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region.
    Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S
    J Immunol, 2015;194(1):113-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  21. Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity.
    Authors: Carrillo-Galvez A, Cobo M, Cuevas-Ocana S, Gutierrez-Guerrero A, Sanchez-Gilabert A, Bongarzone P, Garcia-Perez A, Munoz P, Benabdellah K, Toscano M, Martin F, Anderson P
    Stem Cells, 2015;33(1):183-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  22. Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-beta Signaling in the Retinal Pigment Epithelium.
    Authors: Li Y, Song D, Song Y, Zhao L, Wolkow N, Tobias J, Song W, Dunaief J
    J Biol Chem, 2015;290(19):11918-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  23. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015;10(2):e0118097.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  24. STAT6-Dependent Collagen Synthesis in Human Fibroblasts Is Induced by Bovine Milk.
    Authors: Kippenberger S, Zoller N, Kleemann J, Muller J, Kaufmann R, Hofmann M, Bernd A, Meissner M, Valesky E
    PLoS ONE, 2015;10(7):e0131783.
    Species: Bovine
    Sample Type: Milk
    Application: Neut
  25. Commercial cow milk contains physically stable extracellular vesicles expressing immunoregulatory TGF-beta.
    Authors: Pieters B, Arntz O, Bennink M, Broeren M, van Caam A, Koenders M, van Lent P, van den Berg W, de Vries M, van der Kraan P, van de Loo F
    PLoS ONE, 2015;10(3):e0121123.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  26. Anterior gradient 2 downregulation in a subset of pancreatic ductal adenocarcinoma is a prognostic factor indicative of epithelial-mesenchymal transition.
    Authors: Mizuuchi Y, Aishima S, Ohuchida K, Shindo K, Fujino M, Hattori M, Miyazaki T, Mizumoto K, Tanaka M, Oda Y
    Lab Invest, 2015;95(2):193-206.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  27. Bortezomib inhibits expression of TGF-beta1, IL-10, and CXCR4, resulting in decreased survival and migration of cutaneous T cell lymphoma cells.
    Authors: Chang T, Poltoratsky V, Vancurova I
    J Immunol, 2015;194(6):2942-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  28. STAT3 governs hyporesponsiveness and granzyme B-dependent suppressive capacity in human CD4+ T cells.
    Authors: Schmetterer K, Neunkirchner A, Wojta-Stremayr D, Leitner J, Steinberger P, Pickl W
    FASEB J, 2015;29(3):759-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  29. IL-10 inhibits neuraminidase-activated TGF-beta and facilitates Th1 phenotype during early phase of infection.
    Authors: Dutta A, Huang C, Chen T, Lin C, Chiu C, Lin Y, Chang C, He Y
    Nat Commun, 2015;6(0):6374.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  30. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation.
    Authors: Faustino L, Fonseca D, Florsheim E, Resende R, Lepique A, Faquim-Mauro E, Gomes E, Silva J, Yagita H, Russo M
    Mucosal Immunol, 2014;7(5):1199-208.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  31. Functional effects of TGF-beta1 on mesenchymal stem cell mobilization in cockroach allergen-induced asthma.
    Authors: Gao P, Zhou Y, Xian L, Li C, Xu T, Plunkett B, Huang S, Wan M, Cao X
    J Immunol, 2014;192(10):4560-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  32. CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice.
    Authors: Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W
    Immunology, 2014;141(3):401-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  33. Tenascin-X promotes epithelial-to-mesenchymal transition by activating latent TGF-beta.
    Authors: Alcaraz L, Exposito J, Chuvin N, Pommier R, Cluzel C, Martel S, Sentis S, Bartholin L, Lethias C, Valcourt U
    J Cell Biol, 2014;205(3):409-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  34. Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis.
    Authors: Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H
    J Clin Invest, 2014;124(1):448-60.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  35. Senescent cancer-associated fibroblasts secrete active MMP-2 that promotes keratinocyte dis-cohesion and invasion.
    Authors: Hassona Y, Cirillo N, Heesom K, Parkinson E, Prime S
    Br J Cancer, 2014;111(6):1230-7.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  36. Aminobisphosphonates prevent the inhibitory effects exerted by lymph node stromal cells on anti-tumor Vdelta 2 T lymphocytes in non-Hodgkin lymphomas.
    Authors: Musso A, Catellani S, Canevali P, Tavella S, Vene R, Boero S, Pierri I, Gobbi M, Kunkl A, Ravetti J, Zocchi M, Poggi A
    Haematologica, 2014;99(1):131-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  37. Bovine gammadelta T cells are a major regulatory T cell subset.
    Authors: Guzman E, Hope J, Taylor G, Smith A, Cubillos-Zapata C, Charleston B
    J Immunol, 2014;193(1):208-22.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Bioassay
  38. Biglycan and decorin differentially regulate signaling in the fetal membranes.
    Authors: Wu Z, Horgan C, Carr O, Owens R, Iozzo R, Lechner B
    Matrix Biol, 2014;35(0):266-75.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  39. The extracellular matrix protein MAGP1 supports thermogenesis and protects against obesity and diabetes through regulation of TGF-beta.
    Authors: Craft C, Pietka T, Schappe T, Coleman T, Combs M, Klein S, Abumrad N, Mecham R
    Diabetes, 2014;63(6):1920-32.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  40. Abnormal muscle mechanosignaling triggers cardiomyopathy in mice with Marfan syndrome.
    Authors: Cook J, Carta L, Benard L, Chemaly E, Chiu E, Rao S, Hampton T, Yurchenco P, Costa K, Hajjar R, Ramirez F
    J Clin Invest, 2014;124(3):1329-39.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  41. Basal localization of MT1-MMP is essential for epithelial cell morphogenesis in 3D collagen matrix.
    Authors: Weaver S, Wolters B, Ito N, Woskowicz A, Kaneko K, Shitomi Y, Seiki M, Itoh Y
    J Cell Sci, 2014;127(0):1203-13.
    Species: Canine
    Sample Type: Whole Cells
    Application: Neut
  42. Influenza promotes collagen deposition via alphavbeta6 integrin-mediated transforming growth factor beta activation.
    Authors: Jolly L, Stavrou A, Vanderstoken G, Meliopoulos V, Habgood A, Tatler A, Porte J, Knox A, Weinreb P, Violette S, Hussell T, Kolb M, Stampfli M, Schultz-Cherry S, Jenkins G
    J Biol Chem, 2014;289(51):35246-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  43. The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6.
    Authors: Nagashima, Hiroyuki, Okuyama, Yuko, Asao, Atsuko, Kawabe, Takeshi, Yamaki, Satoshi, Nakano, Hiroyasu, Croft, Michael, Ishii, Naoto, So, Takanori
    Nat Immunol, 2014;15(5):449-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  44. CD2-mediated regulation of peripheral CD4(+) CD25(+) regulatory T-cell apoptosis accompanied by down-regulation of Bim.
    Authors: Kashiwakura Y, Sakurai D, Kanno Y, Hashiguchi M, Kobayashi A, Kurosu A, Tokudome S, Kobata T, Kojima H
    Immunology, 2013;139(1):48-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  45. Chronic but not acute virus infection induces sustained expansion of myeloid suppressor cell numbers that inhibit viral-specific T cell immunity.
    Authors: Norris B, Uebelhoer L, Nakaya H, Price A, Grakoui A, Pulendran B
    Immunity, 2013;38(2):309-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  46. Peyer's patch inducer cells play a leading role in the formation of B and T cell zone architecture.
    Authors: Nakagawa R, Togawa A, Nagasawa T, Nishikawa S
    J Immunol, 2013;190(7):3309-18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  47. Staphylococcus aureus phenol-soluble modulin peptides modulate dendritic cell functions and increase in vitro priming of regulatory T cells.
    Authors: Schreiner J, Kretschmer D, Klenk J, Otto M, Buhring H, Stevanovic S, Wang J, Beer-Hammer S, Peschel A, Autenrieth S
    J Immunol, 2013;190(7):3417-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  48. The metalloprotease ADAM12 regulates the effector function of human Th17 cells.
    Authors: Zhou A, El Hed A, Mercer F, Kozhaya L, Unutmaz D
    PLoS ONE, 2013;8(11):e81146.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  49. A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells.
    Authors: Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, Chen W, Stepkowski S
    J Immunol, 2013;190(7):3153-62.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  50. Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress.
    Authors: Gandhi N, Su Z, Zhang X, Volpe E, Pelegrino F, Rahman S, Li D, Pflugfelder S, de Paiva C
    J Leukoc Biol, 2013;94(6):1293-301.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  51. Human CD1c+ dendritic cells drive the differentiation of CD103+ CD8+ mucosal effector T cells via the cytokine TGF-beta.
    Authors: Yu C, Becker C, Wang Y, Marches F, Helft J, Leboeuf M, Anguiano E, Pourpe S, Goller K, Pascual V, Banchereau J, Merad M, Palucka K
    Immunity, 2013;38(4):818-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  52. TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement.
    Authors: Bialas A, Stevens B
    Nat Neurosci, 2013;16(12):1773-82.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Immunodepletion
  53. TGF-beta signaling to T cells inhibits autoimmunity during lymphopenia-driven proliferation.
    Authors: Zhang N, Bevan MJ
    Nat. Immunol., 2012;13(7):667-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  54. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
    Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
    Nature, 2012;484(7395):514-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  55. Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
    Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB
    J. Clin. Invest., 2011;121(0):3024.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  56. Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance.
    Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA
    J. Immunol., 2011;186(12):6661-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  57. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.
    Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ
    Mol. Biol. Cell, 2011;22(10):1686-98.
    Species: Canine
    Sample Type: Whole Cells
    Application: Neut
  58. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
    Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
    J. Clin. Invest., 2011;121(11):4548-66.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  59. Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-gamma, but not transforming growth factor-beta dependent.
    Authors: Srivastava K, Zhang T, Yang N, Sampson H, Li XM
    Clin. Exp. Allergy, 2010;40(11):1678-88.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  60. Epithelial overexpression of SOCS-3 in transgenic mice exacerbates wound inflammation in the presence of elevated TGF-beta1.
    Authors: Linke A, Goren I, Bosl MR, Pfeilschifter J, Frank S
    J. Invest. Dermatol., 2010;130(3):866-75.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Bioassay
  61. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010;28(5):939-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  62. Nuclear factor-kappaB is a critical mediator of stress-impaired neurogenesis and depressive behavior.
    Authors: Koo JW, Russo SJ, Ferguson D, Nestler EJ, Duman RS
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(6):2669-74.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  63. Interleukin-17-producing gammadelta+ T cells protect NOD mice from type 1 diabetes through a mechanism involving transforming growth factor-beta.
    Authors: Han G, Wang R, Chen G
    Immunology, 2010;129(2):197-206.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  64. Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade.
    Authors: Lu H, Wagner WM, Gad E, Yang Y, Duan H, Amon LM, Van Denend N, Larson ER, Chang A, Tufvesson H, Disis ML
    J. Immunol., 2010;184(9):5360-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  65. Loss of Thy-1 inhibits alveolar development in the newborn mouse lung.
    Authors: Nicola T, Hagood JS, James ML, Macewen MW, Williams TA, Hewitt MM, Schwiebert L, Bulger A, Oparil S, Chen YF, Ambalavanan N
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L738-50.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  66. Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
    Authors: Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM
    J. Immunol., 2009;182(5):2929-38.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  67. Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
    Authors: Prignano F, Campolmi P, Bonan P
    Dermatol Ther, 2009;22(0):S8-15.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  68. The AP-1 transcription factor Batf controls T(H)17 differentiation.
    Authors: Schraml BU, Hildner K, Ise W, Lee WL, Smith WA, Solomon B, Sahota G, Sim J, Mukasa R, Cemerski S, Hatton RD, Stormo GD, Weaver CT, Russell JH, Murphy TL, Murphy KM
    Nature, 2009;460(7253):405-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  69. Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10.
    Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA
    Nat. Immunol., 2009;10(9):981-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  70. CD69+ CD4+ CD25- T cells, a new subset of regulatory T cells, suppress T cell proliferation through membrane-bound TGF-beta 1.
    Authors: Han Y, Guo Q, Zhang M, Chen Z, Cao X
    J. Immunol., 2009;182(1):111-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  71. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  72. Molecular mechanisms of TGFbeta receptor-triggered signaling cascades rapidly induced by the calcineurin inhibitors cyclosporin A and FK506.
    Authors: Akool el-S, Doller A, Babelova A, Tsalastra W, Moreth K, Schaefer L, Pfeilschifter J, Eberhardt W
    J. Immunol., 2008;181(4):2831-45.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  73. CD3-specific antibody-induced immune tolerance involves transforming growth factor-beta from phagocytes digesting apoptotic T cells.
    Authors: Perruche S, Zhang P, Liu Y, Saas P, Bluestone JA, Chen W
    Nat. Med., 2008;14(5):528-35.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  74. Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells.
    Authors: van der Vliet HJ, Wang R, Yue SC, Koon HB, Balk SP, Exley MA
    J. Immunol., 2008;180(11):7287-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  75. Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production.
    Authors: Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L
    Am. J. Pathol., 2008;172(4):1127-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  76. Characterization of naturally occurring CD4+CD25+ regulatory T cells in rhesus monkeys.
    Authors: Haanstra KG, van der Maas MJ, 't Hart BA, Jonker M
    Transplantation, 2008;85(8):1185-92.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Neut
  77. Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling.
    Authors: Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB
    Oncogene, 2008;27(54):6834-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  78. Regulatory T cells control dendritic cell/NK cell cross-talk in lymph nodes at the steady state by inhibiting CD4+ self-reactive T cells.
    Authors: Terme M, Chaput N, Combadiere B, Ma A, Ohteki T, Zitvogel L
    J. Immunol., 2008;180(7):4679-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  79. Imbalance between pSmad3 and Notch induces CDK inhibitors in old muscle stem cells.
    Authors: Carlson ME, Hsu M, Conboy IM
    Nature, 2008;454(7203):528-32.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
  80. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  81. Integrin alpha3beta1 potentiates TGFbeta-mediated induction of MMP-9 in immortalized keratinocytes.
    Authors: Lamar JM, Iyer V, DiPersio CM
    J. Invest. Dermatol., 2007;128(3):575-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  82. Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge.
    Authors: He R, Oyoshi MK, Jin H, Geha RS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(40):15817-22.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  83. In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid.
    Authors: Saurer L, McCullough KC, Summerfield A
    J. Immunol., 2007;179(6):3504-14.
    Species: Porcine
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  84. Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
    Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
    J. Virol., 2007;81(11):5882-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  85. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006;56(2):243-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  86. Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
    Authors: Neurohr C, Nishimura SL, Sheppard D
    Am. J. Respir. Cell Mol. Biol., 2006;35(2):252-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  87. Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells.
    Authors: Dasgupta S, Bhattacharya-Chatterjee M, O&amp;amp;apos;Malley BW, Chatterjee SK
    J. Cell. Biochem., 2006;97(5):1036-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  88. Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation.
    Authors: Fazzini M, Vallejo G, Colman-Lerner A, Trigo R, Campo S, Baranao JL, Saragueta PE
    J. Cell. Physiol., 2006;207(1):40-8.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Neut
  89. Long-term staphylococcal enterotoxin C1 exposure induces soluble factor-mediated immunosuppression by bovine CD4+ and CD8+ T cells.
    Authors: Seo KS, Lee SU, Park YH, Davis WC, Fox LK, Bohach GA
    Infect. Immun., 2006;75(1):260-9.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Neut
  90. IGF-I and IGFBP-3 augment transforming growth factor-beta actions in human renal carcinoma cells.
    Authors: Rosendahl AH, Forsberg G
    Kidney Int., 2006;70(9):1584-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  91. Requirement of Smad3 for mast cell growth.
    Authors: Funaba M, Nakaya K, Ikeda T, Murakami M, Tsuchida K, Sugino H
    Cell. Immunol., 2006;240(1):47-52.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  92. Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytes.
    Authors: Koelle DM, Huang J, Hensel MT, McClurkan CL
    J. Virol., 2006;80(6):2863-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  93. Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling.
    Authors: Close JL, Gumuscu B, Reh TA
    Development, 2005;132(13):3015-26.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  94. Protection against autoimmunity in nonlymphopenic hosts by CD4+ CD25+ regulatory T cells is antigen-specific and requires IL-10 and TGF-beta.
    Authors: Huang X, Zhu J, Yang Y
    J. Immunol., 2005;175(7):4283-91.
    Species: Mouse
    Sample Type:
    Application: Neut
  95. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  96. P311 binds to the latency associated protein and downregulates the expression of TGF-beta1 and TGF-beta2.
    Authors: Paliwal S, Shi J, Dhru U, Zhou Y, Schuger L
    Biochem. Biophys. Res. Commun., 2004;315(4):1104-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  97. TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice.
    Authors: Kitani A, Fuss I, Pedersen A, Nawata H
    J. Immunol., 2004;172(2):834-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  98. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
    Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
    J. Immunol., 2004;172(9):5753-64.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Tissue Homogenates
    Application: WB
  99. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004;104(2):453-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  100. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  101. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
    Authors: Kobayashi T, Iijima K, Kita H
    J. Immunol., 2003;170(11):5756-63.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  102. Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3.
    Authors: Chen, WanJun, Jin, Wenwen, Hardegen, Neil, Lei, Ke-Jian, Li, Li, Marinos, Nancy, McGrady, George, Wahl, Sharon M
    J Exp Med, 2003;198(12):1875-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  103. IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression.
    Authors: Koenen HJ, Fasse E, Joosten I
    J. Immunol., 2003;171(12):6431-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  104. Immunosuppression in hamsters with progressive visceral leishmaniasis is associated with an impairment of protein kinase C activity in their lymphocytes that can be partially reversed by okadaic acid or anti-transforming growth factor beta antibody.
    Authors: Mookerjee A, Sen PC, Ghose AC
    Infect. Immun., 2003;71(5):2439-46.
    Species: Hamster
    Sample Type: Whole Cells
    Application: Neut
  105. The cxc chemokine cCAF stimulates differentiation of fibroblasts into myofibroblasts and accelerates wound closure.
    Authors: Feugate JE, Li Q, Wong L, Martins-Green M
    J. Cell Biol., 2002;156(1):161-72.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Neut
  106. Human macrophages induce CD4(+)Foxp3(+) regulatory T cells via binding and re-release of TGF-beta.
    Authors: Schmidt A, Zhang X, Joshi R, Iqbal S, Wahlund C, Gabrielsson S, Harris R, Tegner J
    Immunol Cell Biol, 0;94(8):747-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  107. A mechanism linking Id2-TGFbeta crosstalk to reversible adaptive plasticity in neuroblastoma.
    Authors: Chakrabarti L, Wang B, Lee N, Sandler A
    PLoS ONE, 0;8(12):e83521.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  108. PARP-1 regulates expression of TGF-beta receptors in T cells.
    Authors: Zhang P, Nakatsukasa H, Tu E, Kasagi S, Cui K, Ishikawa M, Konkel J, Maruyama T, Wei G, Abbatiello B, Wang Z, Zhao K, Chen W
    Blood, 0;122(13):2224-32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 108 Citations
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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TGF-beta 1, 2, 3 Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Average Rating: 4.5 (Based on 2 reviews)

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Immunocytochemistry/Immunofluorescence TGF-beta 1, 2, 3 Antibody MAB1835
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Excellent
 ICC/IF Human Anonymous 07/21/2017
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Immunocytochemistry/Immunofluorescence TGF-beta 1, 2, 3 Antibody MAB1835
Immunocytochemistry/Immunofluorescence: TGF-beta 1, 2, 3 Antibody [MAB1835]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedA549 human lung carcinoma cell line
SpeciesHuman
  Very Good
 Block/Neutralize Mouse Anonymous 05/16/2017
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Summary

ApplicationBlock/Neutralize
Sample TestedPeritoneal macrophages
SpeciesMouse

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