TGF-beta 1, 2, 3 Antibody

Name: TGF-beta 1, 2, 3 Antibody
Brand: R&D Systems
Rating: 4.8 (4 reviews)

(116 citations)

(4 Reviews)

Datasheet / CoA / SDS

Immunocytochemistry

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Immunohistochemistry

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Neutralization

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Product Details

Citations (116)

FAQs

Supplemental Products

Reviews

Specificity

Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
Source

Monoclonal Mouse IgG₁ Clone # 1D11
Purification

Protein A or G purified from hybridoma culture supernatant
Immunogen

Bovine bone-derived TGF-beta 1 and TGF-beta 2
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TGF-beta 1 (Catalog # 240-B) under non-reducing conditions only
Immunohistochemistry

8-25 µg/mL

See below
Immunocytochemistry

8-25 µg/mL

See below

Human TGF-beta 1 Sandwich Immunoassay

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

TGF-beta 1, 2, 3 Antibody (Catalog # MAB1835 )

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

TGF‑ beta 1 Biotinylated Antibody (Catalog # BAF240 )

ELISA Standard

Recombinant Human TGF-beta 1 Protein (Catalog # 240-B )

Neutralization

Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1990) Lymphokine Res. 9:607. The Neutralization Dose (ND₅₀) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Binding Inhibition

Dasch, J.R. et al. (1994) J. Immunol. 142:1536.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry click image to view larger	TGF‑ beta in PC-3 Human Cell Line. TGF‑ beta 1, 2, 3 was detected in immersion fixed PC-3 human prostate cancer cell line using 10 µg/mL Mouse Anti-TGF‑ beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry click image to view larger	TGF-beta in Human Skin. TGF-beta 1, 2, 3 was detected in immersion fixed paraffin-embedded human skin using 25 µg/mL Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835) overnight at 4 °C. Tissues was stained using the Anti-Mouse HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with Haematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization click image to view larger	TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1, 2, 3 Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240‑B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‑ beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835). The ND₅₀ is typically 0.25‑1.25 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References:
1. Ayala A. et al. (1992) FASEB J. 6:A1604.
2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Long Name:

Transforming Growth Factor beta 1, 2, and 3

Alternate Names:

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

116 Citations: Showing 1 - 10
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Species	Applications	Sample Type
Proteomic Analysis of Nucleus Pulposus Cell-derived Extracellular Matrix Niche and Its Effect on Phenotypic Alteration of Dermal Fibroblasts Authors: M Yuan, PJ Pai, X Liu, H Lam, BP Chan Sci Rep, 2018;8(1):1512. Species: Rabbit Sample Type: Whole Cells Application: ICC Downregulation of membrane trafficking proteins and lactate conditioning determine loss of dendritic cell function in lung cancer Authors: N Caronni, F Simoncello, F Stafetta, C Guarnaccia, JS Ruiz-Moren, B Opitz, T Galli, V Proux-Gill, F Benvenuti Cancer Res., 2018;0(0):. Species: Mouse Sample Type: Whole Cells Application: Bioassay Connective tissue growth factor expression after angiotensin II exposure is dependent on transforming growth factor-? signaling via the canonical Smad-dependent pathway in hypertensive induced myocardial fibrosis Authors: CKS Wong, A Falkenham, T Myers, JF Légaré J Renin Angiotensin Aldosterone Syst, 2018;19(1):1470320318759. Species: Mouse Sample Type: Whole Cells Application: Neut The therapeutic HIV Env C5/gp41 vaccine candidate Vacc-C5 induces specific T cell regulation in a phase I/II clinical study Authors: K Brekke, M Sommerfelt, M Ökvist, AM Dyrhol-Rii, D Kvale BMC Infect. Dis, 2017;17(1):228. Species: Human Sample Type: Whole Cells Application: Neut Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function Authors: RM Petri, A Hackel, K Hahnel, CA Dumitru, K Bruderek, SB Flohe, A Paschen, S Lang, S Brandau Stem Cell Reports, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3(+) regulatory T cells Authors: M Pasztoi, J Pezoldt, M Beckstette, C Lipps, D Wirth, M Rohde, K Paloczi, EI Buzas, J Huehn Eur. J. Immunol., 2017;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut ALS skeletal muscle shows enhanced TGF-? signaling, fibrosis and induction of fibro/adipogenic progenitor markers Authors: D Gonzalez, O Contreras, DL Rebolledo, JP Espinoza, B van Zunder, E Brandan PLoS ONE, 2017;12(5):e0177649. Species: Mouse Sample Type: Tissue Homogenates Application: WB Integrin ?1 activation induces an anti-melanoma host response Authors: L Ritsma, I Dey-Guha, N Talele, X Sole, Salony, J Chowdhury, KN Ross, S Ramaswamy PLoS ONE, 2017;12(4):e0175300. Species: Human Sample Type: Whole Cells Application: Functional Assay A new developmental mechanism for the separation of the mammalian middle ear ossicles from the jaw Authors: DJ Urban, N Anthwal, ZX Luo, JA Maier, A Sadier, AS Tucker, KE Sears Proc. Biol. Sci, 2017;284(1848):. Species: Opossum Sample Type: In Vivo Application: In Vivo Histone Deacetylase (HDAC) Inhibition Induces I?B Kinase (IKK)-dependent Interleukin-8/CXCL8 Expression in Ovarian Cancer Cells Authors: HR Gatla, Y Zou, MM Uddin, B Singha, P Bu, A Vancura, I Vancurova J. Biol. Chem., 2017;292(12):5043-5054. Species: Human Sample Type: Cell Lysates Application: WB Mechanosignaling activation of TGF? maintains intervertebral disc homeostasis Authors: Q Bian, L Ma, A Jain, JL Crane, K Kebaish, M Wan, Z Zhang, X Edward Guo, PD Sponseller, CA Séguin, LH Riley, Y Wang, X Cao Bone Res, 2017;5(0):17008. Species: Mouse Sample Type: Whole Tissue Application: Bioassay Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro PLoS ONE, 2016;11(11):e0167410. Species: Human Sample Type: Whole Cells Application: Neut Mammary epithelial cell phagocytosis downstream of TGF-beta3 is characterized by adherens junction reorganization. Authors: Fornetti J, Flanders K, Henson P, Tan A, Borges V, Schedin P Cell Death Differ, 2016;23(2):185-96. Species: Rat Sample Type: Tissue Homogenates Application: WB Apoptotic cell infusion treats ongoing collagen-induced arthritis, even in the presence of methotrexate, and is synergic with anti-TNF therapy Arthritis Res Ther, 2016;18(1):184. Species: Mouse Sample Type: In Vivo Application: In Vivo K+/Cl- cotransporter KCC2 membrane trafficking and functionality is regulated by transforming growth factor beta 2 J Cell Sci, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Bioassay Autonomous and Non-autonomous Defects Underlie Hypertrophic Cardiomyopathy in BRAF-Mutant hiPSC-Derived Cardiomyocytes Stem Cell Reports, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-? signalling Authors: Fatemeh Mirzamoham Nat Commun, 2016;7(0):12047. Species: Mouse Sample Type: In Vivo Application: In vivo TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development Authors: H Nagashima, Y Okuyama, T Hayashi, N Ishii, T So J Immunol, 2016;0(0):. TGF-? Neutralization Enhances AngII-Induced Aortic Rupture and Aneurysm in Both Thoracic and Abdominal Regions Authors: X Chen, DL Rateri, DA Howatt, A Balakrishn, JJ Moorleghen, LA Cassis, A Daugherty PLoS ONE, 2016;11(4):e0153811. Species: Mouse Sample Type: In Vivo Application: In vivo Sterile Inflammation Enhances ECM Degradation in Integrin ?1 KO Embryonic Skin Cell Rep, 2016;16(12):3334-47. Species: Mouse Sample Type: Whole Tissue Application: IHC - PFA fixed TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT) PLoS ONE, 2016;11(9):e0162221. Species: Human Sample Type: Whole Cells Application: Neut Different tissue phagocytes sample apoptotic cells to direct distinct homeostasis programs Nature, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Bioassay Cancer associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-ï¿½-dependent pathways in genotype-specific oral cancer Authors: S S Prime Carcinogenesis, 2016;0(0):. Species: Human Sample Type: Conditioned Media Application: Neturalization Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion PLoS ONE, 2016;11(11):e0166500. Species: Human Sample Type: Whole Cells Application: Neut [64Cu]XYIMSR-06: A dual-motif CAIX ligand for PET imaging of clear cell renal cell carcinoma Authors: I Minn, SM Koo, HS Lee, M Brummet, SP Rowe, MA Gorin, P Sysa-Shah, WD Lewis, HH Ahn, Y Wang, SR Banerjee, RC Mease, S Nimmagadda, ME Allaf, MG Pomper, X Yang Oncotarget, 2016;7(35):56471-56479. Species: Mink Sample Type: Whole Cells Application: Neut Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-beta Signaling in the Retinal Pigment Epithelium. Authors: Li Y, Song D, Song Y, Zhao L, Wolkow N, Tobias J, Song W, Dunaief J J Biol Chem, 2015;290(19):11918-34. Species: Human Sample Type: Whole Cells Application: Neut Bortezomib inhibits expression of TGF-beta1, IL-10, and CXCR4, resulting in decreased survival and migration of cutaneous T cell lymphoma cells. Authors: Chang T, Poltoratsky V, Vancurova I J Immunol, 2015;194(6):2942-53. Species: Human Sample Type: Whole Cells Application: Neut Anterior gradient 2 downregulation in a subset of pancreatic ductal adenocarcinoma is a prognostic factor indicative of epithelial-mesenchymal transition. Authors: Mizuuchi Y, Aishima S, Ohuchida K, Shindo K, Fujino M, Hattori M, Miyazaki T, Mizumoto K, Tanaka M, Oda Y Lab Invest, 2015;95(2):193-206. Species: Human Sample Type: Whole Cells Application: Neut Commercial cow milk contains physically stable extracellular vesicles expressing immunoregulatory TGF-beta. Authors: Pieters B, Arntz O, Bennink M, Broeren M, van Caam A, Koenders M, van Lent P, van den Berg W, de Vries M, van der Kraan P, van de Loo F PLoS ONE, 2015;10(3):e0121123. Species: Mouse Sample Type: Whole Cells Application: Neut STAT6-Dependent Collagen Synthesis in Human Fibroblasts Is Induced by Bovine Milk. Authors: Kippenberger S, Zoller N, Kleemann J, Muller J, Kaufmann R, Hofmann M, Bernd A, Meissner M, Valesky E PLoS ONE, 2015;10(7):e0131783. Species: Bovine Sample Type: Milk Application: Neut Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-beta (TGF-beta) Activation and Fibroblast Differentiation. Authors: Dayer C, Stamenkovic I J Biol Chem, 2015;290(22):13763-78. Species: Human Sample Type: Whole Cells Application: Neut STAT3 governs hyporesponsiveness and granzyme B-dependent suppressive capacity in human CD4+ T cells. Authors: Schmetterer K, Neunkirchner A, Wojta-Stremayr D, Leitner J, Steinberger P, Pickl W FASEB J, 2015;29(3):759-71. Species: Human Sample Type: Whole Cells Application: Blocking Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region. Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S J Immunol, 2015;194(1):113-24. Species: Human Sample Type: Whole Cells Application: Neut Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity. Authors: Carrillo-Galvez A, Cobo M, Cuevas-Ocana S, Gutierrez-Guerrero A, Sanchez-Gilabert A, Bongarzone P, Garcia-Perez A, Munoz P, Benabdellah K, Toscano M, Martin F, Anderson P Stem Cells, 2015;33(1):183-95. Species: Mouse Sample Type: Whole Cells Application: Neut IL-10 inhibits neuraminidase-activated TGF-beta and facilitates Th1 phenotype during early phase of infection. Authors: Dutta A, Huang C, Chen T, Lin C, Chiu C, Lin Y, Chang C, He Y Nat Commun, 2015;6(0):6374. Species: Mouse Sample Type: Whole Cells Application: Neut Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta. Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F PLoS ONE, 2015;10(2):e0118097. Species: Human Sample Type: Whole Cells Application: Neut Senescent cancer-associated fibroblasts secrete active MMP-2 that promotes keratinocyte dis-cohesion and invasion. Authors: Hassona Y, Cirillo N, Heesom K, Parkinson E, Prime S Br J Cancer, 2014;111(6):1230-7. Species: Human Sample Type: Cell Culture Supernates Application: Neut Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis. Authors: Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H J Clin Invest, 2014;124(1):448-60. Species: Mouse Sample Type: In Vivo Application: In vivo Abnormal muscle mechanosignaling triggers cardiomyopathy in mice with Marfan syndrome. Authors: Cook J, Carta L, Benard L, Chemaly E, Chiu E, Rao S, Hampton T, Yurchenco P, Costa K, Hajjar R, Ramirez F J Clin Invest, 2014;124(3):1329-39. Species: Mouse Sample Type: In Vivo Application: In vivo Functional effects of TGF-beta1 on mesenchymal stem cell mobilization in cockroach allergen-induced asthma. Authors: Gao P, Zhou Y, Xian L, Li C, Xu T, Plunkett B, Huang S, Wan M, Cao X J Immunol, 2014;192(10):4560-70. Species: Human Sample Type: Whole Cells Application: Neut Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Authors: Faustino L, Fonseca D, Florsheim E, Resende R, Lepique A, Faquim-Mauro E, Gomes E, Silva J, Yagita H, Russo M Mucosal Immunol, 2014;7(5):1199-208. Species: Mouse Sample Type: In Vivo Application: Neut The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6. Authors: Nagashima, Hiroyuki, Okuyama, Yuko, Asao, Atsuko, Kawabe, Takeshi, Yamaki, Satoshi, Nakano, Hiroyasu, Croft, Michael, Ishii, Naoto, So, Takanori Nat Immunol, 2014;15(5):449-56. Species: Mouse Sample Type: Whole Cells Application: Neut CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice. Authors: Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W Immunology, 2014;141(3):401-15. Species: Mouse Sample Type: Whole Cells Application: Neut Aminobisphosphonates prevent the inhibitory effects exerted by lymph node stromal cells on anti-tumor Vdelta 2 T lymphocytes in non-Hodgkin lymphomas. Authors: Musso A, Catellani S, Canevali P, Tavella S, Vene R, Boero S, Pierri I, Gobbi M, Kunkl A, Ravetti J, Zocchi M, Poggi A Haematologica, 2014;99(1):131-9. Species: Human Sample Type: Whole Cells Application: Neut Basal localization of MT1-MMP is essential for epithelial cell morphogenesis in 3D collagen matrix. Authors: Weaver S, Wolters B, Ito N, Woskowicz A, Kaneko K, Shitomi Y, Seiki M, Itoh Y J Cell Sci, 2014;127(0):1203-13. Species: Canine Sample Type: Whole Cells Application: Neut Influenza promotes collagen deposition via alphavbeta6 integrin-mediated transforming growth factor beta activation. Authors: Jolly L, Stavrou A, Vanderstoken G, Meliopoulos V, Habgood A, Tatler A, Porte J, Knox A, Weinreb P, Violette S, Hussell T, Kolb M, Stampfli M, Schultz-Cherry S, Jenkins G J Biol Chem, 2014;289(51):35246-63. Species: Human Sample Type: Whole Cells Application: Neut Tenascin-X promotes epithelial-to-mesenchymal transition by activating latent TGF-beta. Authors: Alcaraz L, Exposito J, Chuvin N, Pommier R, Cluzel C, Martel S, Sentis S, Bartholin L, Lethias C, Valcourt U J Cell Biol, 2014;205(3):409-28. Species: Human Sample Type: Whole Cells Application: Neut The extracellular matrix protein MAGP1 supports thermogenesis and protects against obesity and diabetes through regulation of TGF-beta. Authors: Craft C, Pietka T, Schappe T, Coleman T, Combs M, Klein S, Abumrad N, Mecham R Diabetes, 2014;63(6):1920-32. Species: Mouse Sample Type: In Vivo Application: In vivo Biglycan and decorin differentially regulate signaling in the fetal membranes. Authors: Wu Z, Horgan C, Carr O, Owens R, Iozzo R, Lechner B Matrix Biol, 2014;35(0):266-75. Species: Mouse Sample Type: Tissue Homogenates Application: WB Bovine gammadelta T cells are a major regulatory T cell subset. Authors: Guzman E, Hope J, Taylor G, Smith A, Cubillos-Zapata C, Charleston B J Immunol, 2014;193(1):208-22. Species: Bovine Sample Type: Whole Cells Application: Bioassay CD2-mediated regulation of peripheral CD4(+) CD25(+) regulatory T-cell apoptosis accompanied by down-regulation of Bim. Authors: Kashiwakura Y, Sakurai D, Kanno Y, Hashiguchi M, Kobayashi A, Kurosu A, Tokudome S, Kobata T, Kojima H Immunology, 2013;139(1):48-60. Species: Mouse Sample Type: Whole Cells Application: Neut Chronic but not acute virus infection induces sustained expansion of myeloid suppressor cell numbers that inhibit viral-specific T cell immunity. Authors: Norris B, Uebelhoer L, Nakaya H, Price A, Grakoui A, Pulendran B Immunity, 2013;38(2):309-21. Species: Mouse Sample Type: Whole Cells Application: Neut Human CD1c+ dendritic cells drive the differentiation of CD103+ CD8+ mucosal effector T cells via the cytokine TGF-beta. Authors: Yu C, Becker C, Wang Y, Marches F, Helft J, Leboeuf M, Anguiano E, Pourpe S, Goller K, Pascual V, Banchereau J, Merad M, Palucka K Immunity, 2013;38(4):818-30. Species: Human Sample Type: Whole Cells Application: Neut Peyer's patch inducer cells play a leading role in the formation of B and T cell zone architecture. Authors: Nakagawa R, Togawa A, Nagasawa T, Nishikawa S J Immunol, 2013;190(7):3309-18. Species: Mouse Sample Type: In Vivo Application: In vivo A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells. Authors: Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, Chen W, Stepkowski S J Immunol, 2013;190(7):3153-62. Species: Mouse Sample Type: Whole Cells Application: Neut Staphylococcus aureus phenol-soluble modulin peptides modulate dendritic cell functions and increase in vitro priming of regulatory T cells. Authors: Schreiner J, Kretschmer D, Klenk J, Otto M, Buhring H, Stevanovic S, Wang J, Beer-Hammer S, Peschel A, Autenrieth S J Immunol, 2013;190(7):3417-26. Species: Human Sample Type: Whole Cells Application: Neut Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress. Authors: Gandhi N, Su Z, Zhang X, Volpe E, Pelegrino F, Rahman S, Li D, Pflugfelder S, de Paiva C J Leukoc Biol, 2013;94(6):1293-301. Species: Mouse Sample Type: In Vivo Application: Neut The metalloprotease ADAM12 regulates the effector function of human Th17 cells. Authors: Zhou A, El Hed A, Mercer F, Kozhaya L, Unutmaz D PLoS ONE, 2013;8(11):e81146. Species: Human Sample Type: Whole Cells Application: Neut TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement. Authors: Bialas A, Stevens B Nat Neurosci, 2013;16(12):1773-82. Species: Mouse Sample Type: Cell Culture Supernates Application: Immunodepletion TGF-beta signaling to T cells inhibits autoimmunity during lymphopenia-driven proliferation. Authors: Zhang N, Bevan MJ Nat. Immunol., 2012;13(7):667-73. Species: Mouse Sample Type: Whole Cells Application: Neut Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Nature, 2012;484(7395):514-8. Species: Human Sample Type: Whole Cells Application: Neut Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease. Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y J. Clin. Invest., 2011;121(11):4548-66. Species: Human Sample Type: Whole Cells Application: ICC Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets. Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB J. Clin. Invest., 2011;121(0):3024. Species: Mouse Sample Type: Whole Cells Application: Flow An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ Mol. Biol. Cell, 2011;22(10):1686-98. Species: Canine Sample Type: Whole Cells Application: Neut Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance. Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA J. Immunol., 2011;186(12):6661-6. Species: Human Sample Type: Whole Cells Application: Neut Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-gamma, but not transforming growth factor-beta dependent. Authors: Srivastava K, Zhang T, Yang N, Sampson H, Li XM Clin. Exp. Allergy, 2010;40(11):1678-88. Species: Mouse Sample Type: In Vivo Application: In vivo Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade. Authors: Lu H, Wagner WM, Gad E, Yang Y, Duan H, Amon LM, Van Denend N, Larson ER, Chang A, Tufvesson H, Disis ML J. Immunol., 2010;184(9):5360-7. Species: Mouse Sample Type: In Vivo Application: In vivo Interleukin-17-producing gammadelta+ T cells protect NOD mice from type 1 diabetes through a mechanism involving transforming growth factor-beta. Authors: Han G, Wang R, Chen G Immunology, 2010;129(2):197-206. Species: Mouse Sample Type: In Vivo Application: In vivo Nuclear factor-kappaB is a critical mediator of stress-impaired neurogenesis and depressive behavior. Authors: Koo JW, Russo SJ, Ferguson D, Nestler EJ, Duman RS Proc. Natl. Acad. Sci. U.S.A., 2010;107(6):2669-74. Species: Mouse Sample Type: In Vivo Application: In vivo Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Authors: Tso GH, Law HK, Tu W Stem Cells, 2010;28(5):939-54. Species: Human Sample Type: Whole Cells Application: Neut Epithelial overexpression of SOCS-3 in transgenic mice exacerbates wound inflammation in the presence of elevated TGF-beta1. Authors: Linke A, Goren I, Bosl MR, Pfeilschifter J, Frank S J. Invest. Dermatol., 2010;130(3):866-75. Species: Mouse Sample Type: Cell Lysates Application: Bioassay CD69+ CD4+ CD25- T cells, a new subset of regulatory T cells, suppress T cell proliferation through membrane-bound TGF-beta 1. Authors: Han Y, Guo Q, Zhang M, Chen Z, Cao X J. Immunol., 2009;182(1):111-20. Species: Mouse Sample Type: Whole Cells Application: Neut Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair. Authors: Prignano F, Campolmi P, Bonan P Dermatol Ther, 2009;22(0):S8-15. Species: Human Sample Type: Whole Tissue Application: IHC The AP-1 transcription factor Batf controls T(H)17 differentiation. Authors: Schraml BU, Hildner K, Ise W, Lee WL, Smith WA, Solomon B, Sahota G, Sim J, Mukasa R, Cemerski S, Hatton RD, Stormo GD, Weaver CT, Russell JH, Murphy TL, Murphy KM Nature, 2009;460(7253):405-9. Species: Mouse Sample Type: Whole Cells Application: Neut Loss of Thy-1 inhibits alveolar development in the newborn mouse lung. Authors: Nicola T, Hagood JS, James ML, Macewen MW, Williams TA, Hewitt MM, Schwiebert L, Bulger A, Oparil S, Chen YF, Ambalavanan N Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L738-50. Species: Mouse Sample Type: In Vivo Application: In vivo Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function. Authors: Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM J. Immunol., 2009;182(5):2929-38. Species: Mouse Sample Type: Whole Cells Application: Neut TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M Eur. J. Immunol., 2009;39(5):1221-30. Species: Human Sample Type: Whole Cells Application: Neut Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10. Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA Nat. Immunol., 2009;10(9):981-91. Species: Mouse Sample Type: Whole Cells Application: Neut Imbalance between pSmad3 and Notch induces CDK inhibitors in old muscle stem cells. Authors: Carlson ME, Hsu M, Conboy IM Nature, 2008;454(7203):528-32. Species: Mouse Sample Type: Whole Tissue Application: Neut CD3-specific antibody-induced immune tolerance involves transforming growth factor-beta from phagocytes digesting apoptotic T cells. Authors: Perruche S, Zhang P, Liu Y, Saas P, Bluestone JA, Chen W Nat. Med., 2008;14(5):528-35. Species: Mouse Sample Type: In Vivo Application: In vivo Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells. Authors: van der Vliet HJ, Wang R, Yue SC, Koon HB, Balk SP, Exley MA J. Immunol., 2008;180(11):7287-93. Species: Human Sample Type: Whole Cells Application: Neut Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production. Authors: Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L Am. J. Pathol., 2008;172(4):1127-40. Species: Human Sample Type: Whole Cells Application: Flow Characterization of naturally occurring CD4+CD25+ regulatory T cells in rhesus monkeys. Authors: Haanstra KG, van der Maas MJ, 't Hart BA, Jonker M Transplantation, 2008;85(8):1185-92. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Cells Application: Neut Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling. Authors: Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB Oncogene, 2008;27(54):6834-44. Species: Human Sample Type: Whole Cells Application: Neut Molecular mechanisms of TGFbeta receptor-triggered signaling cascades rapidly induced by the calcineurin inhibitors cyclosporin A and FK506. Authors: Akool el-S, Doller A, Babelova A, Tsalastra W, Moreth K, Schaefer L, Pfeilschifter J, Eberhardt W J. Immunol., 2008;181(4):2831-45. Species: Rat Sample Type: In Vivo Application: In vivo Regulatory T cells control dendritic cell/NK cell cross-talk in lymph nodes at the steady state by inhibiting CD4+ self-reactive T cells. Authors: Terme M, Chaput N, Combadiere B, Ma A, Ohteki T, Zitvogel L J. Immunol., 2008;180(7):4679-86. Species: Mouse Sample Type: Whole Cells Application: Neut Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Integrin alpha3beta1 potentiates TGFbeta-mediated induction of MMP-9 in immortalized keratinocytes. Authors: Lamar JM, Iyer V, DiPersio CM J. Invest. Dermatol., 2007;128(3):575-86. Species: Mouse Sample Type: Whole Cells Application: Neut Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses. Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ J. Virol., 2007;81(11):5882-92. Species: Human Sample Type: Whole Cells Application: Neut In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid. Authors: Saurer L, McCullough KC, Summerfield A J. Immunol., 2007;179(6):3504-14. Species: Porcine Sample Type: Cell Culture Supernates Application: ELISA Development Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge. Authors: He R, Oyoshi MK, Jin H, Geha RS Proc. Natl. Acad. Sci. U.S.A., 2007;104(40):15817-22. Species: Mouse Sample Type: In Vivo Application: In vivo IGF-I and IGFBP-3 augment transforming growth factor-beta actions in human renal carcinoma cells. Authors: Rosendahl AH, Forsberg G Kidney Int., 2006;70(9):1584-90. Species: Human Sample Type: Whole Cells Application: Neut Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S Gut, 2006;56(2):243-52. Species: Human Sample Type: Whole Cells Application: Neut Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK Dev. Biol., 2006;295(2):473-85. Species: Rat Sample Type: Whole Tissue Application: Neut Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure. Authors: Neurohr C, Nishimura SL, Sheppard D Am. J. Respir. Cell Mol. Biol., 2006;35(2):252-9. Species: Human Sample Type: Whole Cells Application: Neut Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation. Authors: Fazzini M, Vallejo G, Colman-Lerner A, Trigo R, Campo S, Baranao JL, Saragueta PE J. Cell. Physiol., 2006;207(1):40-8. Species: Bovine Sample Type: Whole Cells Application: Neut Long-term staphylococcal enterotoxin C1 exposure induces soluble factor-mediated immunosuppression by bovine CD4+ and CD8+ T cells. Authors: Seo KS, Lee SU, Park YH, Davis WC, Fox LK, Bohach GA Infect. Immun., 2006;75(1):260-9. Species: Bovine Sample Type: Whole Cells Application: Neut Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells. Authors: Dasgupta S, Bhattacharya-Chatterjee M, O&amp;apos;Malley BW, Chatterjee SK J. Cell. Biochem., 2006;97(5):1036-51. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytes. Authors: Koelle DM, Huang J, Hensel MT, McClurkan CL J. Virol., 2006;80(6):2863-72. Species: Human Sample Type: Whole Cells Application: Neut Requirement of Smad3 for mast cell growth. Authors: Funaba M, Nakaya K, Ikeda T, Murakami M, Tsuchida K, Sugino H Cell. Immunol., 2006;240(1):47-52. Species: Mouse Sample Type: Cell Lysates Application: WB Protection against autoimmunity in nonlymphopenic hosts by CD4+ CD25+ regulatory T cells is antigen-specific and requires IL-10 and TGF-beta. Authors: Huang X, Zhu J, Yang Y J. Immunol., 2005;175(7):4283-91. Species: Mouse Sample Type: Application: Neut Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling. Authors: Close JL, Gumuscu B, Reh TA Development, 2005;132(13):3015-26. Species: Rat Sample Type: In Vivo Application: In vivo Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates. Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ J. Immunol., 2004;172(9):5753-64. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Tissue Homogenates Application: WB Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Immunology, 2004;112(2):274-89. Species: Human Sample Type: Whole Cells Application: Flow In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures. Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB Blood, 2004;104(2):453-61. Species: Human Sample Type: Whole Cells Application: Flow P311 binds to the latency associated protein and downregulates the expression of TGF-beta1 and TGF-beta2. Authors: Paliwal S, Shi J, Dhru U, Zhou Y, Schuger L Biochem. Biophys. Res. Commun., 2004;315(4):1104-9. Species: Mouse Sample Type: Cell Lysates Application: WB TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice. Authors: Kitani A, Fuss I, Pedersen A, Nawata H J. Immunol., 2004;172(2):834-42. Species: Human Sample Type: Whole Cells Application: Neut Immunosuppression in hamsters with progressive visceral leishmaniasis is associated with an impairment of protein kinase C activity in their lymphocytes that can be partially reversed by okadaic acid or anti-transforming growth factor beta antibody. Authors: Mookerjee A, Sen PC, Ghose AC Infect. Immun., 2003;71(5):2439-46. Species: Hamster Sample Type: Whole Cells Application: Neut Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3. Authors: Chen, WanJun, Jin, Wenwen, Hardegen, Neil, Lei, Ke-Jian, Li, Li, Marinos, Nancy, McGrady, George, Wahl, Sharon M J Exp Med, 2003;198(12):1875-86. Species: Mouse Sample Type: Whole Cells Application: Neut Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice. Authors: Kobayashi T, Iijima K, Kita H J. Immunol., 2003;170(11):5756-63. Species: Mouse Sample Type: In Vivo Application: In vivo IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression. Authors: Koenen HJ, Fasse E, Joosten I J. Immunol., 2003;171(12):6431-41. Species: Human Sample Type: Whole Cells Application: Neut The cxc chemokine cCAF stimulates differentiation of fibroblasts into myofibroblasts and accelerates wound closure. Authors: Feugate JE, Li Q, Wong L, Martins-Green M J. Cell Biol., 2002;156(1):161-72. Species: Chicken Sample Type: Whole Cells Application: Neut PARP-1 regulates expression of TGF-beta receptors in T cells. Authors: Zhang P, Nakatsukasa H, Tu E, Kasagi S, Cui K, Ishikawa M, Konkel J, Maruyama T, Wei G, Abbatiello B, Wang Z, Zhao K, Chen W Blood, 0;122(13):2224-32. Species: Mouse Sample Type: Whole Cells Application: Flow Human macrophages induce CD4(+)Foxp3(+) regulatory T cells via binding and re-release of TGF-beta. Authors: Schmidt A, Zhang X, Joshi R, Iqbal S, Wahlund C, Gabrielsson S, Harris R, Tegner J Immunol Cell Biol, 0;94(8):747-62. Species: Human Sample Type: Whole Cells Application: Neut A mechanism linking Id2-TGFbeta crosstalk to reversible adaptive plasticity in neuroblastoma. Authors: Chakrabarti L, Wang B, Lee N, Sandler A PLoS ONE, 0;8(12):e83521. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Expand to show all Citations

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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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TGF-beta 1, 2, 3 Antibody

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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Basic

VisUCyte Antigen Retrieval Reagent-Acidic

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Mouse IgG1 Isotype Control

Mouse IgG1 Isotype Control

TGF-beta 1, 2, 3 Antibody

Reconstitution Buffer 1 (PBS)

Mouse IgG HRP-conjugated Antibody

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Mouse IgG HRP-conjugated Antibody

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Donkey Anti-Mouse IgG Biotinylated Antibody

Mouse IgG Antibody

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Goat Anti-Mouse IgG Biotinylated Antibody

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

Mouse IgG VisUCyte HRP Polymer Antibody

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Donkey Anti-Mouse IgG (H+L) Affinity Purified PAb, X Absorb

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Mouse IgG₁ Isotype Control

Mouse IgG₁ Isotype Control