Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TLR9 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9. The immunogen is located within amino acids 840 - 890 of TLR9. Amino Acid Squence: RGRQSGRDEDALPYD

Specificity

At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TLR9 Antibody - BSA Free (NBP1-76680) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-TLR9 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TLR9 Antibody - BSA Free

Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]

Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]

TLR9-Antibody-Western-Blot-NBP1-76680-img0008.jpg
TLR9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] -

Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] - Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 ug/mL.
Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]

Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]

Western Blot: TLR9 Antibody [NBP1-76680] - Analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide
Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]

Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]

TLR9-Antibody-Western-Blot-NBP1-76680-img0007.jpg
TLR9 Antibody - BSA Free

Western Blot: TLR9 Antibody - BSA Free [NBP1-76680] -

The expression levels of microtubule protein light chain 3 (LC3B), PTEN inducing putative kinase 1 (PINK1), Parkin, mitofusin 1 (Mfn1), toll-like receptor (TLR) 9, cytochrome c oxidase 4 (COX4), myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON) or mechanical ventilation at high tidal volume (HTV) with saline, antimycin A (AmA) or cyclosporine A (CsA). (A) Levels of PINK1, Parkin, & Mfn1 mRNA. (B) Levels of LC3B, PINK1, Parkin, & Mfn1 protein by Western blot. (C) Relative expression of LC3B-II/LC3B-I & PINK1 protein. (D) Relative expression of Parkin & Mfn1 protein. (E) Levels of TLR9 & COX4 mRNA. (F) Levels of MyD88 & nuclear factor-kappa B (NF-kappa B) mRNA. (G) Levels of TLR9, COX4, MyD88, & NF-kappa B protein by Western blot. (H) Relative expression of TLR9 & COX4 protein. (I) Relative expression of MyD88 & NF-kappa B protein. Fold expression for target genes was normalized to that measured for the beta -actin gene. Both of these experiments were in triplicate. aP < 0.05 vs. CON group;bP < 0.05 vs. HTV group; & cP < 0.05 vs. AmA group. Image collected & cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TLR9 Antibody - BSA Free

Western Blot: TLR9 Antibody - BSA Free [NBP1-76680] -

Western Blot: TLR9 Antibody - BSA Free [NBP1-76680] - Levels of toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON), mechanical ventilation at normal tidal volume (NTV), high tidal volume (HTV), & ODN2088 pretreatment upon HTV stimulation (HTV + ODN). aP < 0.05 vs. CON group;bP < 0.05 vs. NTV group; & cP < 0.05 vs. HTV group. Image collected & cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TLR9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] -

Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] - Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 ug/mL.

Applications for TLR9 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-20 ug/mL

Western Blot

1 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-76680 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

UniProt

AAH32713 (Human)

Additional TLR9 Products

Product Documents for TLR9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TLR9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TLR9 Antibody - BSA Free

Customer Reviews for TLR9 Antibody - BSA Free (1)

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  • TLR9 Antibody
    Name: Prashant Rai
    Application: Western Blot
    Sample Tested: Bone Marrrow Derived Macrophages
    Species: Mouse
    Verified Customer | Posted 07/17/2019
    BMDM#1: Lane 1 untreated and 2 treated with Interferon gamma BMDM#2: Lane 3 untreated and 4 treated with Interferon gamma
    Samples ran on Bis-Tris gradient gel.
    TLR9 Antibody - BSA Free NBP1-76680

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