Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-173

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Mouse, Rat, Mammal

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Tyrosine Hydroxylase Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyrosine Hydroxylase conjugated to KLH. Accession # P04177

Reactivity Notes

Reactivity assumed based on sequence identity to a wide variety of mammalian and non-mammalian species.

Modification

p Ser40

Marker

Neuronal Marker

Specificity

Specific for the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free (NB300-173) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Tyrosine Hydroxylase Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.
Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173]

Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173]

Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.

Applications for Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000

Reviewed Applications

Read 1 review rated 4 using NB300-173 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tyrosine Hydroxylase

The tetrameric enzyme tyrosine hydroxylase (TH), also designated as tyrosine 3-monooxygenase (TY3H), is the rate-limiting enzyme for catecholamine biosynthesis and has a theoretical molecular weight of 60 kDa. Encoded by the TH gene, TH catalyzes the conversion of the amino acid L-tyrosine to L-3,4- dihydroxyphenylalanine (L-dopa) in the central nervous system and adrenal medulla. The precursor of catecholamines, L-dopa, is converted to dopamine for use in the biosynthesis of neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline) (1). Dysfunction in the TH gene, and thus the enzymatic activity by TH, dysregulates catecholamine synthesis.

Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. Dysregulation of breakdown for the amino acid, tyrosine, by TH is a result of a genetic disorder that results in Tyrosinemia (high levels of tyrosine in the blood, tissue and organs).

Tyrosine hydroxylase deficiency is a disorder that primarily affects movement, where individuals display symptoms that include lack of coordination when walking, postural tremors and unusual body positioning. TH deficient dopamine-responsive dystonia (DRD), also known as Segawa syndrome, is a rare genetic disorder that is associated with low levels of TH and is diagnosed during childhood with characteristic symptoms including increased muscle tone (dystonia) and signs of Parkinsonism like bradykinesia, tremors, rigidity and postural instability (2). Correspondingly, TH is also linked to Parkinson's disease in older adults, where low dopamine levels are a consistent neurochemical abnormality. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia and other affective disorders where dopamine is often dysregulated (3).

References

1. Hamanaka, Y., & Mizunami, M. (2019). Tyrosine hydroxylase-immunoreactive neurons in the mushroom body of the field cricket, Gryllus bimaculatus. Cell Tissue Res, 376(1), 97-111. doi:10.1007/s00441-018-2969-9

2. Li, L., & Zhou, F. M. (2013). Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice. Neuroscience, 236, 66-76. doi:10.1016/j.neuroscience.2012.12.065

3. Borkar, C. D., Bharne, A. P., Nagalakshmi, B., Sakharkar, A. J., Subhedar, N. K., & Kokare, D. M. (2018). Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms. Neuroscience, 375, 94-107. doi:10.1016/j.neuroscience.2018.01.056

Alternate Names

TH, TYH

Entrez Gene IDs

7054 (Human); 21823 (Mouse); 25085 (Rat)

Gene Symbol

TH

UniProt

P04177 (Rat)

Additional Tyrosine Hydroxylase Products

Product Documents for Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

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  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: mouse brain
    Species: Mouse
    Verified Customer | Posted 03/23/2010

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