Detects viral CCI in direct ELISAs and Western blots.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant viral CCI
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Viral CCI Fc Chimera (Catalog # 696-CC)
Measured by its ability to neutralize CCI inhibition of CCL2/JE/MCP‑1-dependent chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 0.4 µg/mL Recombinant Viral CCI Fc Chimera and 20 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CCI Inhibition of CCL2/JE-dependent Chemotaxis and Neutralization by Viral CCI Antibody. Recombinant Viral CCI Fc Chimera (Catalog # 696‑CC) inhibits Recombinant Mouse CCL2/JE (Catalog # 479‑JE) induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Mouse CCL2/JE (20 ng/mL) activity elicited by Recombinant Viral CCI Fc Chimera (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral CCI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF696). The ND50 is typically 3-15 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The family of T1/35 kDa proteins are secreted, soluble proteins encoded by the 35K virulence gene of many poxviruses. These proteins bind CC chemokines with high affinity and are now termed viral chemokine inhibitors (vCCI). Vaccinia virus (Strain Lister) vCCI shows greater than 90% amino acid sequence identity with vCCI from other orthopoxviruses and approximately 40% amino acid sequence identity with the leporipoxvirus T-1 proteins.
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