Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2230C
View all HIV-1 Gag p24 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant viral HIV-1 Gag p24 
Pro149-Gly354
Accession # Q77YG1

Specificity

Detects viral HIV-1 Gag p24 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Viral HIV‑1 Gag p24 Antibody

Detection of HIV-1 Gag p24 antibody by Western Blot.

Detection of HIV‑1 Gag p24 by Western Blot.

Western blot shows recombinant HIV Gag p24. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB9539) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIV-1 Gag p24 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral HIV‑1 Gag p24 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant HIV Gag p24

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

UniProt

Q77YG1

Additional HIV-1 Gag p24 Products

Product Documents for Viral HIV‑1 Gag p24 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Viral HIV‑1 Gag p24 Antibody

For research use only

Related Research Areas

Infectious Diseases

Citations for Viral HIV‑1 Gag p24 Antibody

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