Viral wnvNS1 Protein Antibody Summary
Accession # NP_776015
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of West Nile Virus (wnv) NS1 Protein by Western Blot. Western blot shows recombinant West Nile Virus (wnv) NS1 protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB29072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for wnvNS1 Protein at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: wnvNS1 Protein
West Nile Virus, or WNV, is a member of the Flaviviridae family that includes other human pathogens such as yellow fever (YF), dengue (DEN), tick-borne encephalitis (TBE), Japanese encephalitis (JEV), and Murray Valley encephalitis (MVE) viruses. West Nile virus (WNV) is a single-stranded RNA mosquito-transmitted virus that causes epidemics of encephalitis and viscerotropic disease. The WNV nonstructural protein NS1 is a 48-kDa highly conserved secreted glycoprotein that does not package with the virion. Even when absent from the virion, immunization with NS1 typically elicits a protective immune response.
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